中国水稻科学

• 研究简报 • 上一篇    

用单克隆抗体酶联免疫吸附测定法检测水稻种子上的白叶枯病菌

李清铣; 刘斯文; 高锦梁; 胡广淦; 朱华; 黄奔立   

  1. 江苏农学院,扬州225001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1992-06-05 发布日期:1992-06-05

The Detection of Xanthomonas campestris pv. oryzae with the McAb-ELISA after Multiplication

Li Qingxian; Li Shiwen; Gao Jinliang; Hu Guanggan; Zhu Hua; Huang Benli   

  • Received:1900-01-01 Revised:1900-01-01 Online:1992-06-05 Published:1992-06-05

关键词: 种子, 水稻, 白叶枯病, 单克隆抗体, 免疫吸附测定法

Abstract: A double antibodies sandwich ELISA with the monoclonal antibody as the second antibody for the rapid detection of Xanthomonas campestris pv. oryzae in rice seeds was developed. The bacterial from husk of the seed samples were shake-cultured in sterile liquid medium for 48 h at 25℃, and followed by ELISA detection. 126 seed samples from Jiangsu and Zhejiang were tested, 93% of seed samples from infected rice were positive.

Key words: ELISA test, Monoclonal antibody, seed, Xanthomonas campestris pv. oryzae