中国水稻科学

中国水稻科学

• 实验技术 • 上一篇    下一篇

水稻蛋白质的双向电泳分析技术

杨 炜1； 毕学知2；黄大年1； 肖翊华2   

  1. 1 中国水稻研究所，杭州310006；2 武汉大学，武汉430072
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1993-01-10 发布日期:1993-01-10

A Method for the Two-dimensional Gel Electrophoresis of Rice Proteins

Yang Wei; Bi Xuezhi; Huang Danian; Xiao Yuhua   

  • Received:1900-01-01 Revised:1900-01-01 Online:1993-01-10 Published:1993-01-10

PDF

被引次数

25

摘要： 在O'Farre11(1975)和Anderson(1978)等的基础上对双向电泳方法进行改进得到了一种适合于分析水稻种子胚、黄化芽、绿色幼苗及成熟叶片等不同组织蛋白质的双向电电泳方法。用这一方法对15个水稻品种的种子胚、黄化芽、二叶期绿色幼苗及雌雄蕊形成期成熟叶片的蛋白质进行双向电泳分离，经考马斯亮蓝R250染色后，均获得了清晰而稳定的图谱。以200 μg左右的蛋白质进行电泳，从上述组织可分辩出的蛋白质(多肽)点分别选捌550、500和350个。由此建议在分子水平上研究水稻等禾谷类作物的遗传学、发育生理学以及品种资源鉴定时采用本方祛。

关键词: 水稻, 蛋白质, 双向电泳, 实验方法

Abstract: An improved procedure for the two-dimensional electrophoresis of proteins from rice (Oryza sativa L.)is presented based on the methods of O'Farrell (1975) and Anderson (1978). The two-dimensional separation of proteins from different tissues including seed embryo, etiolated bud, green seedling and mature leaf provides distinct and steady results, and the number of protein (peptide) spots detected after coomassie brilliant blue R-250 staining with about 200 μg of proteins from these tissues are up to 550. 500 and 350, respectively. This method will be suitable for the identification of rice germplasm and can also be used to study systematically the genetics and developmental physiology of rice and other cereals at molecular level.

Key words: Protein, Rice, Two-dimensional gei electrophoresis, Methodology