中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

利用水稻糯与非糯(近)等基因系探讨WX基因对成熟胚培养力的影响

郑 柔；奚永安；林贻滋   

  1. 浙江省农业科学院，杭州310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1993-01-10 发布日期:1993-01-10

Effect of wx Gene on Mature Embryo Culture Ability in Waxy and Non-waxy Isogenic Rice Lines

Zheng Lou; Xi Yong'an; Lin Yizi   

  • Received:1900-01-01 Revised:1900-01-01 Online:1993-01-10 Published:1993-01-10

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摘要： 采用6对糯与非糯(近)等基因系（3籼3粳）研究了基因对成熟胚培养力的影响。糯与非糯(近)等基因系之间愈伤组织诱导率和生长速率存在极显著差异，并且这种差异高于籼粳类型造成的差异；糯与非糯(近)等基因系的绿苗分化率与上述两种性状呈相反趋势，差异也达到显著水平。直链淀粉的含量与愈伤组织诱导率、生长速率、绿苗分化率的相关均未达到显著水平。表明wx 基因不仅控制直链淀粉含量，而且还具有提 提高愈伤组织诱导率和生长速率、降低绿苗分化率的多效作用。

关键词: 等基因系, 组织培养, 糯性基因, 水稻

Abstract: Six waxy and non-waxy isogenic lines (3 indica and 3 japonica) were nsed to analyse the effect of wx gene on mature embryo culture ability in rice. It was found that the differences of the callus induced percentage and its growth rate between waxy and non-waxy isogenic lines were significant and even higher in indica than in japonica. The significant difference of green plantlet redifferentiation percentage was also found between waxy and non-waxy isogenic lines, but the wx gene have a different effect on callus induced percentage and growth rate as well as on green plantlet redifferentiation percentage. The amylose content was not related to the callus induced percentage, growth rate or green plantlet redifferentiation percentage. It is suggested that the wx gene has a multiple actions such as controlling the amylose content, promoting the callus induced percentage and growth rate and reducing the green plantlet redifferentiation percentage.

Key words: Isogenic line, Mature embryo, Somatic tissue culture, wx gene