水稻野败型三系杂交种纯度检测功能标记的开发与应用

doi:10.16819/j.1001-7216.2026.250204

中国水稻科学 ›› 2026, Vol. 40 ›› Issue (2): 244-252.DOI: 10.16819/j.1001-7216.2026.250204

水稻野败型三系杂交种纯度检测功能标记的开发与应用

张梦柯¹^,^#, 陆佳雨²^,^#, 何金³, 许学², 吴爽², 王沛然², 陈若凡², 金青¹^,^*(), 汪秀峰²^,^*()

¹安徽农业大学，生命科学学院，合肥 230036
²安徽省农业科学院水稻研究所/农业农村部农作物生态环境安全检验测试中心（合肥），合肥 230031
³六安市农业科学研究院，安徽六安 237000

收稿日期:2025-02-14 修回日期:2025-07-15 出版日期:2026-03-10 发布日期:2026-03-16
通讯作者: * email: qingjin@ahau.edu.cn; xiufengwang66@sohu.com
作者简介:^#共同第一作者
基金资助:
安徽省科技攻坚计划项目(重大项目)(202423m10050002);水稻种质创新与分子改良安徽省重点实验室开放基金资助项目(SDKF-2024-05)

Development and Application of Functional Markers for Purity Identification of Wild-abortive Three-line Hybrid Rice

ZHANG Mengke¹^,^#, LU Jiayu²^,^#, HE Jin³, XU Xue², WU Shuang², WANG Peiran², CHEN Ruofan², JIN Qing¹^,^*(), WANG Xiufeng²^,^*()

¹School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
²Rice Research Institute, Anhui Academy of Agricultural Sciences/Crop Ecological Environment Safety Inspection and Testing Center (Hefei), Ministry of Agriculture and Rural Affairs, Hefei 230031, China
³Lu'an Academy of Agricultural Sciences, Lu'an 237000, China

Received:2025-02-14 Revised:2025-07-15 Online:2026-03-10 Published:2026-03-16
Contact: * email: qingjin@ahau.edu.cn; xiufengwang66@sohu.com
About author:sup>#These authors contributed equally to this work

1. zhangmengke Table S1.docx(17KB)

摘要/Abstract

摘要：

【目的】开发稳定、高效、可靠的三系杂交种纯度检测标记，对于当前三系水稻制种而言至关重要。【方法】根据野败型不育基因WA352、主效恢复基因Rf4和内参基因Actin的特异性序列，设计了多对单基因检测标记，并通过调整引物混合比例，进行PCR扩增。【结果】引物WA352-2、Rf4-3和Actin-3按照1∶11∶3的比例混合可实现单次PCR扩增同时检测WA352、Rf4和Actin的基因类型，区分野败型不育系、恢复系、保持系和杂交种。【结论】本研究开发的检测标记操作方法简便，结果稳定可靠，不仅能够检测野败型三系水稻的基因型，还可以作为分子标记辅助育种的有效工具，提高育种过程中纯度检测结果的准确性，从而为我国水稻高产优质育种目标的实现提供有力支持。

关键词: 水稻, 野败, 三系, 检测标记, PCR

Abstract:

【Objective】 Developing stable, efficient, and reliable markers for purity testing of three-line hybrid seeds is crucial for current hybrid rice seed production. 【Method】 Based on the specific sequences of the wild abortive-type sterility gene WA352, the major fertility-restoring gene Rf4, and the internal reference gene Actin, multiple single-gene detection markers were designed. PCR amplification was then performed by optimizing the primer mixture ratio. 【Result】 A mixture of primers WA352-2, Rf4-3, and Actin-3 in a ratio of 1:11:3 enabled simultaneous detection of the genotypes of WA352, Rf4, and Actin in a single PCR reaction, allowing differentiation among wild abortive-type CMS lines, restorer lines, maintainer lines, and hybrids. 【Conclusion】 The detection method established in this study is simple to perform and yields stable and reliable results. It can not only identify genotypes in wild-abortive three-line rice but also serve as an effective tool for molecular marker-assisted breeding. By improving the accuracy of purity assessment during breeding, this method provides strong support for achieving high-yield and high-quality rice breeding objectives in China.

Key words: rice, wild-abortive, three-line, detection marker, PCR

张梦柯, 陆佳雨, 何金, 许学, 吴爽, 王沛然, 陈若凡, 金青, 汪秀峰. 水稻野败型三系杂交种纯度检测功能标记的开发与应用[J]. 中国水稻科学, 2026, 40(2): 244-252.

ZHANG Mengke, LU Jiayu, HE Jin, XU Xue, WU Shuang, WANG Peiran, CHEN Ruofan, JIN Qing, WANG Xiufeng. Development and Application of Functional Markers for Purity Identification of Wild-abortive Three-line Hybrid Rice[J]. Chinese Journal OF Rice Science, 2026, 40(2): 244-252.

图/表 8

表1 PCR扩增引物序列信息

Table 1. Sequence of PCR amplification primers

引物名称 Primer name	引物序列 Primer sequence	目标基因 Target gene	目的条带大小 Target band size(bp)
WA352-1	F: GTGCTTAAATCACTACAGGAGCG R: CCCTGGAAGCGGTTGATTGA	WA352	528
WA352-2	F: GAGGATTTTGCCTCCCCTCC R: GGTGCAACCTAGCCAAGTCT	WA352	604
WA352-3	F: GCTTACGCTATGGAGAACCCT R: TTCCCCCGTGCTTTCTTGA	WA352	93
Rf4-1	F: CTTTAGTTCAATAATTAGCGATCT R: TGACATTGGGCTTCACACCA	Rf4	101
Rf4-2	F: CTTTAGTTCAATAATTAGCGATCT R: ACCAGCTAAGCAGCATCCAT	Rf4	142
Rf4-3	F: CTTTAGTTCAATAATTAGCGATCT R: ACTAACGCGTCTTCCATCCT	Rf4	267
Actin-1	F: GCTCCTGAGGAACATCCAATTT R: AGCCACATACATTGCTGGTG	Actin	117
Actin-2	F: GGTTTGATTCTTATACCATTGTCCA R: GGGCATCATCACCAGCAAAA	Actin	115
Actin-3	F: GAGGAACATCCAATTTTGCTGA R: CACATACATTGCTGGTGCATT	Actin	108

图1 目的基因WA352上引物结合位点及WA352引物扩增结果 A为目的基因WA352上引物结合位点，括号中数字是以DNA序列5’端第1个碱基为起点，引物在基因组序列上的坐标； B为WA352引物的扩增结果，1: 不育系恒丰A；2: 恢复系银占3号；3: 杂交种荃优丝苗；4: 保持系沪旱7B; PN: 引物名; M: DL2000 DNA 标记(100~2000 bp)。

Fig. 1. Schematic diagram of primer binding sites on the target gene WA352 and WA352 primer amplification results A, Primer binding sites on the target gene WA352. The numbers in parentheses represent the coordinates of the primers on the genomic sequence, with the first base at the 5' end of the DNA sequence as the starting point; B, Amplification results of WA352 primers. 1, CMS line Hengfeng A; 2, Restorer line Yinzhan 3; 3, Hybrid variety Quanyousimiao; 4, Maintainer line Huhan7 B; PN, Primer name; M, DL2000 DNA marker (100-2000 bp).

图2 目的基因Rf4上引物结合位点以及Rf4引物扩增结果 A: 目的基因Rf4上引物结合位点，括号中数字是以DNA序列5’端第1个碱基为起点，引物在基因组序列上的坐标，针对Rf4的三条特异性引物均采用相同的F端引物；B: Rf4引物扩增结果。1: 不育系恒丰A；2: 恢复系银占3号；3: 杂交种荃优丝苗；4: 保持系沪旱7B; PN: 引物名; M: DL2000 DNA 标记 (100~2000 bp)。

Fig. 2. Schematic diagram of primer binding sites on the target gene Rf4 and Rf4 primer amplification results A, Primer binding sites on the target gene Rf4. The numbers in parentheses represent the coordinates of the primers on the genomic sequence, with the first base at the 5' end of the DNA sequence as the starting point. The three specific primers for Rf4 adopt the same F-primer; B, Amplification results of Rf4 primers; 1, CMS line Hengfeng A; 2, Restorer line Yinzhan 3; 3, Hybrid variety Quanyousimiao; 4, Maintainer line Huhan 7B; PN, Primer name;M, DL2000 DNA marker (100-2000 bp).

图3 目的基因Actin上引物结合位点及Actin引物扩增结果 A: 目的基因Actin上引物结合位点示意图，括号中数字是以DNA序列5’端第1个碱基为起点，引物在基因组序列上的坐标；B: Actin引物扩增结果; 1: 不育系恒丰A；2: 恢复系银占3号；3: 杂交种荃优丝苗；4: 保持系沪旱7B。PN: 引物名; M: DL2000 DNA标记(100~2000 bp)。

Fig. 3. Schematic diagram of primer binding sites on the target gene Actin and amplification results of Actin primers A, Primer binding sites on the target gene Rf4. The numbers in parentheses represent the coordinates of the primers on the genomic sequence, with the first base at the 5' end of the DNA sequence as the starting point; B, Amplification results of Actin primers; 1, CMS line Hengfeng A; 2, Restorer line Yinzhan 3; 3, Hybrid variety Quanyousimiao; 4, Maintainer line Huhan 7B; PN, Primer name; M, DL2000 DNA marker (100-2000 bp).

图4 引物组合的初次筛选结果 1: 不育系恒丰A；2: 恢复系银占3号；3: 杂交种荃优丝苗；4: 保持系沪旱7B；PR: 引物WA352-2、Rf4-3、Actin-3的比例; M: DL2000 DNA 标记(100~2000 bp)。

Fig. 4. Initial screening results of primer combinations 1, CMS line Hengfeng A; 2, Restorer line Yinzhan 3; 3, Hybrid Quanyousimiao; 4, Maintainer line Huhan 7B; PR, Primer ratio of WA352-2, Rf4-3 and Actin-3; M, DL2000 DNA marker (100-2000 bp).

图5 引物组合的方法优化结果 A: 第一次引物组合比例优化结果；B: 第二次引物组合比例优化结果，1: 不育系恒丰A；2: 恢复系银占3号；3: 杂交种荃优丝苗；4: 保持系沪旱7B；PR: 引物WA352-2：Rf4-3：Actin-3，M-DL2000 DNA 标记(100-2000 bp)。

Fig. 5. Optimization results of primer combinations A, First primer combination ratio optimization results; B, Second primer combination ratio optimization results, 1, CMS line Hengfeng A; 2, Restorer line Yinzhan 3; 3, Hybrid Quanyousimiao; 4, Maintainer line Huhan 7B; PR, Primer ratio of WA352-2, Rf4-3 and Actin-3; M, DL2000 DNA marker (100-2000 bp).

图6 WRA-F/R引物在不同水稻材料中的扩增情况 A: 以恢复系水稻基因组DNA为模板。泳道1: 银占3号；2: 岳恢9712；3: 乐占；4: 美香新占；5: R2117；6: 绿丝苗；7: 粤禾丝苗；B：以不育系水稻基因组DNA为模板。其中, 泳道8: 恒丰A；9: 沪旱7A；10: 龙特甫A；11: 荷丰A；编号12，明太A；编号13，五丰A；编号14，广8A；C：以杂交种水稻基因组DNA为模板,其中泳道编号15，荃广优4号；16: 荃优丝苗；17: 深优粤禾丝苗；18: 五优晶丝苗；19: 宜优粤禾丝苗；20: 筑优110；21: 荃早优丝苗；D：以常规水稻基因组DNA为模板。其中, 泳道22，中花11；23: 广陆矮4号；24: 蜀恢498；25: 日本晴；26: 9311； 27: 台中65；28: 南京11; 24: 蜀恢498为恢复系; M: DL2000 DNA Marker (100-2000 bp)。

Fig. 6. Amplification results of WRA-F/R primers in different rice materials A, Using genomic DNA from restorer line rice as the template. Lane 1, Yizhan 3; Lane 2, Yuehui 9712; Lane 3, Yuezhan; Lane 4, Meixiangxinzhan; Lane 5, R2117; Lane 6, Lvsimiao; Lane 7, Yuehesimiao; B: Using genomic DNA from CMS line rice as the template. Lane 8 is Hengfeng A ; Lane 9, Huhan7A; Lane 10, Longtefu A; Lane 11, Hefeng A; Lane 12, Mingtai A；Lane 13,WufengA; Lane 14, Guang8A; C, Using genomic DNA from hybrid rice as the template, lane Lane 15, Quanguangyou 4; Lane 16, Quanyousimiao; Lane 17, Shenyouyuehesimiao; Lane 18, Wuyoujingsimiao; Lane 19, Yiyouyuehesimiao；Lane 20, Zhuyou 110; Lane 21, Quanzaoyousimiao; D, Using genomic DNA from conventional rice as the template, Lane 22 is Zhonghua11; Lane 23, Guangluai4; Lane 24, Shuhui498; Lane 25, Nipponbare; Lane 26, 9311；Lane 27, Taizhong 65; Lane 28, Nanjing 11, where Shuhui 498 is the restorer line, M, DL2000 DNA marker (100-2000 bp).

图7 WRA-F/R引物对野败型三系水稻杂交种样品的单株琼脂糖凝胶电泳检测结果 A: 每条泳道对应的DNA均来自样品1育苗后随机抽取的192株幼苗中的一株；B: 每条泳道对应的DNA均来自样品2随机抽取的192株幼苗的一株; M: DL2000 DNA 标记 (100~2000 bp)。

Fig. 7. Detection results of WRA-F/R primers on single-plant agarose gel electrophoresis of the wild-abortive three-line hybrid rice samples A, DNA profile of Sample #1, with each lane corresponding to DNA extracted from one of 192 randomly selected rice seedlings post-nursery cultivation. B, DNA profile of Sample #2, with each lane corresponding to DNA extracted from one of 192 randomly selected rice seedlings. M, DL2000 DNA marker (100-2000 bp).

参考文献 27

[1]	Fukagawa N K, Ziska L H. Rice: Importance for global nutrition[J]. Journal of Nutritional Science and Vitaminology, 2019, 65(Suppl.): S2-S3.
[2]	杨紫涵, 安增旭, 吴殿星, 张宁. 水稻雄性不育的研究和应用进展[J]. 核农学报, 2025, 39(3): 531-545.
	Yang Z H, An Z X, Wu D X, Zhang N. Research and application progress on male sterility of rice[J]. Journal of Nuclear Agricultural Sciences, 2025, 39(3): 531-545. (in Chinese with English abstract)
[3]	Zheng W J, Ma Z B, Zhao M Z, Xiao M M, Zhao J M, Wang C H, Gao H, Bai Y J, Wang H, Sui G M. Research and development strategies for hybrid japonica rice[J]. Rice, 2020, 13(1): 36.
[4]	Chen L T, Liu Y G. Male sterility and fertility restoration in crops[J]. Annual Review of Plant Biology, 2014, 65(1): 579-606.
[5]	李伟, 徐霞, 边莹, 张晓波, 范炯炯, 程本义, 杨仕华, 吴建利, 魏鑫, 曾波, 龚俊义. 5460个三系杂交稻品种的不育系细胞质来源分析[J]. 中国水稻科学, 2024, 38(6): 653-664.
	Li W, Xu X, Bian Y, Zhang X B, Fan J J, Cheng B Y, Yang S H, Wu J L, Wei X, Zeng B, Gong J Y. Cytoplasmic source analysis of sterile lines from 5460 three-line hybrid rice varieties[J]. Chinese Journal of Rice Science, 2024, 38(6): 653-664. (in Chinese with English abstract)
[6]	Luo D P, Xu H, Liu Z L, Guo J X, Li H Y, Chen L T, Fang C, Zhang Q Y, Bai M, Yao N, Wu H, Wu H, Ji C H, Zheng H Q, Chen Y L, Ye S, Li X Y, Zhao X C, Li R Q, Liu Y G. A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice[J]. Nature Genetics, 2013, 45(5): 573-577.
[7]	Tang H, Luo D, Zhou D, Zhang Q Y, Tian D S, Zheng X M, Chen L T, Liu Y G. The rice restorer Rf4 for wild-abortive cytoplasmic male sterility encodes a mitochondrial-localized PPR protein that functions in reduction of WA352 transcripts[J]. Molecular Plant, 2014, 7(9): 1497-1500.
[8]	Zhao Z, Ding Z, Huang J, Meng H J, Zhang Z X, Gou X, Tang H W, Xie X R, Ping J Y, Xiao F M, Liu Y G, Xie Y Y, Chen L T. Copy number variation of the restorer Rf4 underlies human selection of three-line hybrid rice breeding[J]. Nature Communications, 2023, 14(1): 7333.
[9]	Chen Z, Wu J, Deng X W, Tang X Y. Establishment and advances of third-generation hybrid rice technology: A review[J]. Rice, 2023, 16(1): 56.
[10]	黄伟, 丁举高, 刘怡, 曹玉洪. 水稻田块出现“纯度问题”的调查与思考[J]. 中国种业, 2020(3): 38-40.
	Huang W, Ding J G, Liu Y, Cao Y H. Investigation and reflection on “purity problem” in rice field[J]. China Seed Industry, 2020(3): 38-40. (in Chinese)
[11]	Waza S A. Maintenance of genetic purity within the cms lines of rice hybrids using molecular markers[J]. Trends in Biosciences, 2015, 8(12): 3029-3030.
[12]	宋丰顺, 倪大虎, 倪金龙, 李莉杨, 剑波. 杂交水稻种子纯度检测方法综述[J]. 江苏农业科学, 2016, 44(6): 6-11.
	Song F S, Ni D H, Ni J L, Li L Y, Jian B. Study on detection methods of seed purity of hybrid rice: a review[J]. Jiangsu Agricultural Sciences, 2016, 44(6): 6-11. (in Chinese)
[13]	金名捺, 丘式浚, 严维, 谢刚, 邓汉超, 唐晓艳. 水稻温敏雄性核不育基因tms5功能分子标记的开发与应用[J]. 分子植物育种, 2018, 16(17): 5644-5652.
	Jin M N, Qiu S J, Yan W, Xie G, Deng H C, Tang X Y. Development and application of functional molecular marker for thermo-sensitive genic male sterile gene tms5 in rice[J]. Molecular Plant Breeding, 2018, 16(17): 5644-5652. (in Chinese with English abstract)
[14]	Zhang H G, Li X X, Xu Z P, Zhao X Q, Wan Z H, Cheng X J, Liu Q Q, Gu M H, Tang S Z. The effects of Rf4and the genetic mechanism behind fertility restoration of wild abortive cytoplasmic male sterility (WA-CMS) in japonica rice (Oryza sativa ssp. japonica)[J]. Rice, 2022, 15(1): 59.
[15]	Zhang H G, Che J L, Ge Y S, Pei Y, Zhang L J, Liu Q Q, Gu M H, Tang S Z. Ability of Rf5and Rf6 to restore fertility of Chinsurah Boro II-type cytoplasmic male sterile Oryza sativa (ssp. japonica) lines[J]. Rice, 2017, 10(1): 2.
[16]	Zhang H G, Wang R X, Xu Z P, Zhao X Q, Gao H L, Liu Q Q, Tang S Z. The effects of Rf5and Rf6 on fertility restoration in Honglian-type cytoplasmic male sterile (CMS) lines of japonica rice (Oryza sativa L. ssp. japonica)[J]. Molecular Breeding, 2021, 41(10): 64.
[17]	Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA[J]. Nucleic Acids Research, 1980, 8(19): 4321-4325.
[18]	Su N, Hu M L, Wu D X, Wu F Q, Fei G L, Lan Y, Chen X L, Shu X L, Zhang X, Guo X P, Cheng Z J, Lei C L, Qi C K, Jiang L, Wang H Y, Wan J M. Disruption of a rice pentatricopeptide repeat protein causes a seedling-specific albino phenotype and its utilization to enhance seed purity in hybrid rice production[J]. Plant Physiology, 2012, 159(1): 227-238.
[19]	Keasinee T, Sriprapai C, Numphet S, Thiwawan W, Natjaree P, Khanittha D, Kannika S, Supaporn J, Suniyom T, Chuanchom D, Amorntip M. Development of high yielding two-line hybrid rice in Thailand[J]. ScienceAsia, 2021, 47: 153-161.
[20]	陈星, 高子厚. DNA分子标记技术的研究与应用[J]. 分子植物育种, 2019, 17(6): 1970-1977.
	Chen X, Gao Z H. The study and application of DNA molecular marker technique[J]. Molecular Plant Breeding, 2019, 17(6): 1970-1977. (in Chinese with English abstract)
[21]	Hasan N, Choudhary S, Naaz N, Sharma N, Laskar R A. Recent advancements in molecular marker-assisted selection and applications in plant breeding programmes[J]. Journal, Genetic Engineering & Biotechnology, 2021, 19(1): 128.
[22]	徐颖, 郑炜, 吴薇, 荣德福. RAPD技术在泰国香米品种纯度检测中的应用[J]. 安徽农业科学, 2011, 39(4): 1939-1941+1946.
	Xu Y, Zheng W, Wu W, Rong D F. The application of RAPD technology in the purity detection of Thai fragrant rice varieties[J]. Journal of Anhui Agricultural Sciences, 2011, 39(4): 1939-1941+1946. (in Chinese with English abstract)
[23]	黄成志, 雷树凡, 胡景涛, 吕直文, 黄文章, 严明建, 冉彦秀. 利用优化的SSR分子标记技术检测杂交水稻万优481纯度[J]. 安徽农业科学, 2014, 42(22): 7321-7322.
	Huang C Z, Lei S F, Hu J T, Lv Z W, Huang W Z, Yan M J, Ran Y X. Identification and purity test of hybrid rice wanyou481 with optimized SSR markers[J]. Journal of Anhui Agricultural Sciences, 2014, 42(22): 7321-7322. (in Chinese with English abstract)
[24]	Lu C, Liu J, Jiang J, Caleb M B, Tan H L, Masahiko I, Hong D L. Development and application of SCAR markers for discriminating cytoplasmic male sterile lines from their cognate maintainer lines in Indica rice[J]. Rice Science, 2013, 20(3): 191-199.
[25]	田海燕, 张海娜, 王永强, 周永萍, 张莹璐. SNP分子标记及其在作物品种鉴定中的应用[J]. 中国农学通报, 2024, 40(6): 115-121.
	Tian H Y, Zhang H N, Wang Y Q, Zhou Y P, Zhang Y L. SNP Molecular markers: research and its application in crop variety identification[J]. Chinese Agricultural Science Bulletin, 2024, 40(6): 115-121. (in Chinese with English abstract)
[26]	Anjana B, Ray P C, Veena P, Asit B M. Assessment of genetic purity in rice (Oryza sativa L.) hybrids using microsatellite markers[J]. 3 Biotech, 2016, 6(1): 50.
[27]	孙统庆, 陈斌, 汤义华, 邵培珺, 陈永梅, 吴连勇, 喻俊杰. 浅谈江苏杂交水稻种子纯度海南田间种植鉴定[J]. 中国稻米, 2020, 26(2): 60-62.
	Sun T Q, Chen B, Tang Y H, Shao P J, Chen Y M, Wu L Y, Yu J J. Talking about field planting identification of Jiangsu hybrid rice seed purity in Hainan[J]. China Rice, 2020, 26(2): 60-62. (in Chinese with English abstract)

水稻野败型三系杂交种纯度检测功能标记的开发与应用

Development and Application of Functional Markers for Purity Identification of Wild-abortive Three-line Hybrid Rice

RichHTML

PDF

补充材料

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 27

相关文章 15

编辑推荐

Metrics

[1]	薛炮, 王友霜, 何弯弯, 黄晨博, 张涵, 丁震乾, 陈秋丽, 范运新, 丁成伟, 孙廉平, 胡婷婷. 水稻颖壳不闭合基因SG5的鉴定与克隆[J]. 中国水稻科学, 2026, 40(2): 210-222.
[2]	杨大兵, 杜雪树, 李进波, 夏明元, 胡亮, 石桓, 万丙良. 水稻抽穗期调控的分子机理及育种应用进展[J]. 中国水稻科学, 2026, 40(2): 145-154.
[3]	倪晨, 张家豪, 朱昌进, 徐继伟, 胡秋倩, 霍中洋, 戴其根, 许轲, 李国辉. 水稻源流库形成与调控及其影响因素研究进展[J]. 中国水稻科学, 2026, 40(2): 155-170.
[4]	王梦宁, 谢可冉, 高逖, 王真梅, 熊栋梁, 崔克辉. 高温对水稻粒重形成的影响及其栽培调控研究进展[J]. 中国水稻科学, 2026, 40(2): 171-180.
[5]	罗肖郧, 郑兴飞, 彭宣国, 余启芝, 董华林, 殷得所, 王红波, 胡建林, 薛莲, 胡鹏, 徐得泽. 水稻抗倒伏研究：现状、挑战与未来方向[J]. 中国水稻科学, 2026, 40(2): 181-195.
[6]	段敏, 谢留杰, 岳雅妮, 黄善军. 基于CRISPR/Cas9技术创制优质香味粳稻品系[J]. 中国水稻科学, 2026, 40(2): 235-243.
[7]	李兴沂, 陈玲, 邵建韬, 肖素勤, 李金璐, 付惠仙, 殷富有, 张建红, 程在全, 刘丽. 水稻产量与淀粉品质协同调控的分子遗传研究进展[J]. 中国水稻科学, 2026, 40(1): 1-17.
[8]	岳轩宇, 谢文亚, 冯志明, 陈宗祥, 胡珂鸣, 左示敏. OsERF93参与调控水稻纹枯病抗性的研究[J]. 中国水稻科学, 2026, 40(1): 37-50.
[9]	王轶欣, 林参, 马刘洋, 陈龙, 奉保华, 倪深, 魏祥进, 贺记外, 陈天晓. 谷丙转氨酶基因OsAlaAT4调控水稻氮素吸收和产量[J]. 中国水稻科学, 2026, 40(1): 51-60.
[10]	黄奇娜, 姜鸿瑞, 杨婕, 于坤宇, 杨长登, 梁燕. 种子休眠基因Sdr4的生物信息学分析与分子标记开发和应用[J]. 中国水稻科学, 2026, 40(1): 61-71.
[11]	程朝平, 何旎清, 白康呈, 林少俊, 黄凤凰, 刘军化, 程祖锌, 黄成志, 杨德卫. 聚合稻瘟病抗性基因Pigm-1和Pid2的水稻三系不育系福梦A的选育与利用[J]. 中国水稻科学, 2026, 40(1): 72-84.
[12]	刘亚萍, 董译词, 郑君妍, 邱绚, 刘鹏程, 叶亚峰, 刘斌美, 陈析丰, 马伯军. 水稻类病变早衰突变体lmes7的鉴定与基因精细定位[J]. 中国水稻科学, 2026, 40(1): 85-94.
[13]	谢世民, 周誉株, 薛晓迪, 朱广飞, 孙良, 陈建能. 水稻钵苗取栽协同作业式移栽机构设计与试验[J]. 中国水稻科学, 2026, 40(1): 131-144.
[14]	陈玲, 林文英, 梁丽梅, 欧阳由男, 叶胜海, 季芝娟. 水稻开花习性及其在粳型三系不育系选育中的应用[J]. 中国水稻科学, 2025, 39(6): 731-743.
[15]	王娟, 吴丽娟, 洪海波, 姚志文, 王磊, 鄂志国. 水稻泛素结合酶E2的生物学功能研究进展[J]. 中国水稻科学, 2025, 39(6): 744-750.