中国水稻科学 ›› 2018, Vol. 32 ›› Issue (4): 349-356.DOI: 10.16819/j.1001-7216.2018.7131

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水稻温敏转绿突变体osv15的鉴定和遗传分析

齐岳翰1,2, 李瑞莉2,3, 王芳2, 刘洪家2,*(), 易可可4, 朱诚1,*()   

  1. 1中国计量大学 生命科学学院, 杭州 310018
    2浙江省农业科学院 病毒学与生物技术研究所 / 省部共建国家重点实验室培育基地―浙江省植物有害生物防控重点实验室, 杭州 310021
    3浙江师范大学 化学与生命科学学院, 浙江 金华 321000
    4中国农业科学院 农业资源与农业区划研究所, 北京 100081
  • 收稿日期:2017-11-01 修回日期:2018-02-06 出版日期:2018-07-10 发布日期:2018-07-10
  • 通讯作者: 刘洪家,朱诚
  • 基金资助:
    国家自然科学基金资助项目(31571633);浙江省自然科学基金资助项目(LY17C150008)

Identification and Genetic Analysis of Thermo-sensitive Mutant osv15 in Rice

Yuehan QI1,2, Ruili LI2,3, Fang WANG2, Hongjia LIU2,*(), Keke YI4, Cheng ZHU1,*()   

  1. 1College of Life Science, China Jiliang University, Hangzhou 310018, China
    2State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease / Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    3College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    4Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2017-11-01 Revised:2018-02-06 Online:2018-07-10 Published:2018-07-10
  • Contact: Hongjia LIU, Cheng ZHU

摘要:

【目的】本研究旨在鉴定和克隆水稻温敏转绿新基因,揭示其参与叶绿体发生发育和光合作用的分子机制,为高光效育种提供理论支撑。【方法】利用辐射诱变的方法,从粳稻品种日本晴中筛选获得叶片黄化突变体osv15,并对其表型、农艺性状和遗传方式进行详细分析。构建了突变体与Kasalath的F2群体,利用多态性分子标记对目的基因进行定位和测序分析。【结果】osv15幼苗期在22℃低温下叶片黄化,叶绿素含量仅为野生型的10%,光化学效率下降,叶绿体结构异常;随着温度的升高,osv15的叶色由黄转绿,30℃时叶绿素含量恢复到野生型的68%,光化学效率和叶绿体发育与野生型相近。在自然环境下,osv15突变体从苗期至成熟期均表现为叶片黄化,且株高、分蘖数和产量等农艺性状与野生型相比差异显著。遗传分析表明osv15突变体的表型由一对隐性核基因控制。将OsV15基因定位到第6染色体多态性标记S4和S5之间84 kb的区间内,定位区间测序发现突变体中编码分子伴侣蛋白的基因Cpn60β1(LOC_Os06g02380)发生单碱基缺失。【结论】osv15是一个新的水稻温敏转绿突变体,Cpn60β1可能为突变基因。

关键词: 水稻, 温敏, 叶绿体, 基因定位

Abstract:

:【Objective】The objective of the research is to identify and clone a new thermo-sensitive gene of rice, and to reveal the molecular mechanism involved in the process of chloroplast development and photosynthesis, providing theoretical support for photosynthetic efficiency breeding. 【Method】A yellow leaf mutant osv15 was obtained from rice cultivar Nipponbare (abbreviated as Nip) mutagenized by irradiation. The phenotypic, agronomic traits and genetic patterns of osv15 were analyzed in detail, and F2 population was constructed by crossing the mutant with Kasalath, and mutant individuals were selected to map the gene OsV15.【Result】The mutant osv15 exhibited etiolation at seedling stage, with only ten percent of the chlorophyll content of the wild type, declined photochemical efficiency and abnormal structure of chloroplasts under low temperature condition (22℃). As temperature rose, leaf color of the mutant regreened due to increased chlorophyll content, which recovered to sixty-eight percent of that of the wild type under higher temperature condition (30℃). In natural environment, osv15 showed leaf yellowing from seedling stage to maturity stage, and significant differences in plant height, tiller number, seed setting rate and so on. Genetic analysis revealed that the mutant phenotype of osv15 was controlled by a single recessive gene. The gene was located in the 84 kb region of chromosome 6, between molecular markers S4 and S5. Sequencing analysis in this region showed that Cpn60β1(LOC_Os06g02380) that encodes a putative chaperonin 60 beta precursor, carried a single base deletion in osv15.【Conclusion】osv15 is a new thermo-sensitive mutant, and Cpn60β1 may be the mutant gene.

Key words: rice, thermo-sensitive, chloroplast, gene mapping

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