中国水稻科学 ›› 2015, Vol. 29 ›› Issue (1): 22-27.DOI: 10.3969/j.issn.1001-7216.2015.01.003

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水稻新裂颖突变体sg1的遗传分析与基因定位

曾生元1,2, 郭旻1, 李荣德1, 盛生兰2, 龚红兵2, 严长杰1,*()   

  1. 1扬州大学 江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室, 江苏 扬州 225009
    2江苏丘陵地区镇江农业科学研究所, 江苏 句容 212400
  • 收稿日期:2014-04-24 修回日期:2014-06-10 出版日期:2015-01-10 发布日期:2015-01-10
  • 通讯作者: 严长杰
  • 基金资助:
    国家自然科学基金资助项目(31171158);农业部转基因专项(2011ZX08009-003-005);江苏省自然科学基金资助项目(BK2012684;BK20141291);江苏省六大人才高峰项目;江苏高校优势学科建设工程项目;江苏省农业科技自主创新资金资助项目[CX(13)5073]

Genetic Analysis and Fine Mapping for a Novel split glume Mutant in Rice

Sheng-yuan ZENG1,2, Min GUO1, Rong-de LI1, Sheng-lan SHENG2, Hong-bin GONG2, Chang-jie YAN1,*()   

  1. 1Jiangsu Key Laboratory of Crop Genetics and Physiology/ Key Laboratory of Plant Functional Genomics, Ministry of Education of China, Agricultural College of Yangzhou University, Yangzhou 225009, China
    2Zhenjiang Institute of Agricultural Sciences of the Ning-Zhen Hilly District, Jurong 212400, China
  • Received:2014-04-24 Revised:2014-06-10 Online:2015-01-10 Published:2015-01-10
  • Contact: Chang-jie YAN

摘要:

从中籼3037辐射突变体库中获得一个颖花开裂突变体(split glume1, sg1),表型分析发现该突变体不仅颖壳异常,且伴随着生育期提前,植株变矮,颖花退化等现象,结实率也显著降低。遗传分析和分子生物学分析结果表明该突变受1对隐性核基因控制,并最终将其定位在水稻第1染色体上分子标记M1-z21与M1-z27之间,物理距离约为47 kb,这是一种控制颖花发育的新基因,通过预测发现该区段内存在6个开放阅读框。

关键词: 水稻, 花器官, 颖花开裂突变体, 精细定位

Abstract:

A split glume mutant is from the mutant library of indica variety Zhongxian 3037 radiated by 60Co γ-ray. Compared to its wild type, the mutant has not only split glumes, but also characterized by early heading, lower plant height, reduced seed setting rate and degenerated spikelets, which implies that the mutation has a pleiotropic effect. Genetic and molecular analysis indicated that the mutant was controlled by a recessive nuclear gene, temporally designated as split glume 1 (sg1), and then SG1 was fine mapped to a 47-kb DNA region between the STS markers M1-z21 and M1-z27 on 1, where six ORFs in total were predicted. On the basis of the location of SG1, it is deduced that SG1 is a novel gene regulating the rice glume development.

Key words: rice, flower organ, sg1, fine mapping

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