水稻叶片形态相关基因RL3(t)的遗传分析和精细定位

doi:10.3969/j.issn.1001-7216.2014.05. 002

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (5): 458-464.DOI: 10.3969/j.issn.1001-7216.2014.05. 002

水稻叶片形态相关基因RL3(t)的遗传分析和精细定位

郭旻,李荣德,姚健,朱娟,范祥云,王伟,汤述翥,顾铭洪,严长杰^*

扬州大学农学院/江苏省作物遗传生理国家重点实验室培育点/教育部植物功能基因组学重点实验室，江苏扬州 225009；

收稿日期:2014-01-17 修回日期:2014-03-06 出版日期:2014-09-10 发布日期:2014-09-10
通讯作者: 严长杰*
基金资助:
国家自然科学基金资助项目(31171158)；农业部转基因专项(2011ZX08009003005)；江苏省自然科学基金资助项目(BK2012684)；江苏省六大人才高峰项目；江苏高校优势学科建设工程资助项目。

Fine Mapping of Gene RL3(t), Responsible for Leaf Shape Formation in Rice

GUO Min, LI Rongde, YAO Jian, ZHU Juan, FAN Xiangyun, WANG Wei, TANG Shuzhu, GU Minghong, YAN Changjie^*

Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou 225009, China;

Received:2014-01-17 Revised:2014-03-06 Online:2014-09-10 Published:2014-09-10
Contact: YAN Changjie*

摘要/Abstract

摘要： 利用60Coγ射线辐射籼稻品种9311，获得了两份卷叶突变体rl3(t)1和 rl3(t)2。与野生型9311相比，两个突变体叶片显著内卷，株高降低, 穗长变短，并且结实率降低。细胞学分析表明，突变体叶片中泡状细胞数量的减少和形态的改变，可能是导致叶片卷曲的原因之一。遗传分析和等位性测验表明突变体受一对隐性核基因控制，且rl3(t)1和 rl3(t)2等位。以卷叶突变体rl3(t)1与武运粳8号杂交的F2分离群体作为定位群体，利用SSR标记和新发展的STS标记，将RL3(t)基因初步定位于第3染色体长臂上STS标记M345和SSR标记RM6676之间，遗传距离分别为5.5和4.4 cM。进一步扩大定位群体，结合新发展的STS分子标记，最终将该卷叶基因定位在STS标记S339和S336之间，该区段物理距离为46 kb。

关键词: 水稻(Oryza sativa L.), 卷叶, RL3(t), 基因定位

Abstract: Two mutants with rolled leaves, derived from the indica cultivar 9311 via the radiation of 60Coγ ray in M2 generation, temporally designated as rl3(t)1 and rl3(t)2, were served as materials for exploring the mechanism underlying the rolling leaf characteristic. Morphological analysis showed that, these two mutants have typically adaxially rolled leaves. In addition, when compared with wild type 9311, the plant heights and panicle lengths of rl3(t)1 and rl3(t)2 significantly decreased, as well as the seed setting percentage. Cytological analysis suggests that the rolled leaf phenotype may be caused by the change of number and size of bulliform cells. Genetic analysis indicated that rolled leaf character is controlled by a recessive nuclear gene. Crossing between the two mutants, the F1\[rl3(t)1/rl3(t)2\] plants exhibit rolled leaf, suggesting they are allelic. To map the RL3(t) gene, an F2 population was generated by crossing the rl3(t)1 mutants with Wuyunjing 8 as a mapping population. By using simple sequence repeat (SSR) markers and some new designed sequence tagged site (STS) markers, RL3(t) was initially mapped in the region between the STS marker M345 and the SSR marker RM6676 with the genetic distances of 5.5 cM and 4.4 cM, respectively, on the long arm of chromosome 3. Furthermore, with the enlarged population and more developed STS markers, RL3(t) gene was finally delimited to a 46kb long region governed by the STS markers S339 and S336.

Key words: rice (Oryza sativa L.), rolled leaf, RL3(t), gene mapping

中图分类号:

郭旻,李荣德,姚健,朱娟,范祥云,王伟,汤述翥,顾铭洪,严长杰*. 水稻叶片形态相关基因RL3(t)的遗传分析和精细定位[J]. 中国水稻科学, 2014, 28(5): 458-464.

GUO Min, LI Rongde, YAO Jian, ZHU Juan, FAN Xiangyun, WANG Wei, TANG Shuzhu, GU Minghong, YAN Changjie*. Fine Mapping of Gene RL3(t), Responsible for Leaf Shape Formation in Rice[J]. Chinese Journal of Rice Science, 2014, 28(5): 458-464.

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水稻叶片形态相关基因RL3(t)的遗传分析和精细定位

Fine Mapping of Gene RL3(t), Responsible for Leaf Shape Formation in Rice

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