中国水稻科学 ›› 2014, Vol. 28 ›› Issue (2): 111-118.DOI: 10.3969/j.issn.1001-7216.2014.02.001

• 研究报告 •    下一篇

水稻苗期低温白叶突变体cde2的鉴定和基因定位

魏祥进1,# , 宋建1,2,# ,刘胜1,邵高能1,圣忠华1,唐绍清1 ,胡培松1,*   

  1. 1中国水稻研究所 水稻生物学国家重点实验室, 杭州 310006; 2浙江省农业科学院, 杭州310021;
  • 收稿日期:2013-11-15 修回日期:2013-12-12 出版日期:2014-03-10 发布日期:2014-03-10
  • 通讯作者: 胡培松1,*
  • 基金资助:

    国家自然科学基金资助项目(31201193,31201195); 浙江省自然科学基金资助项目(LY12C13003)。

Identification and Genetic Mapping of a Thermosensitive White Leaf Mutant in Rice

WEI Xiangjin 1, # , SONG Jian 1, 2, # , LIU Sheng 1 , SHAO Gaoneng 1 , SHENG Zhonghua 1 , TANG Shaoqing 1 , HU Peisong 1,*   

  1. 1 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 2 Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
  • Received:2013-11-15 Revised:2013-12-12 Online:2014-03-10 Published:2014-03-10
  • Contact: HU Peisong1,*

摘要: 从粳稻品种Asominori的组培后代中发现了一个温度敏感的叶绿素缺乏突变体。低温(23℃)条件下该突变体幼苗3叶期前表现为白化表型随后致死,但在正常温度条件下与野生型无明显差异(30℃)。与野生型相比,该突变体幼苗低温条件下叶绿素含量明显下降,叶绿体结构发育异常。遗传分析结果表明,该突变体受一对隐性核基因控制,定名为cde2(chlorophyll deficient 2)基因。从cde2与籼稻品种培矮64衍生的F2群体中挑选1064株表现为突变表型的单株进行基因定位,将该基因初步定位于水稻第1染色体的着丝粒附近,随后利用已有的SSR标记和自行开发的Indel标记,进一步将该基因定位在标记RM11041和Indel1之间,物理距离为365.6 kb。此外,对该突变体叶绿素合成、光合作用以及质体转录/翻译系统相关基因的表达量测定表明,CDE2突变后增加了与叶绿素合成和质体转录/翻译相关基因的表达,但降低了光合作用相关基因的表达。结果表明,CDE2在水稻叶绿素合成以及叶绿体的发育过程中起着重要的作用。

关键词: 水稻, 叶绿素缺乏, 温度敏感突变, 基因定位

Abstract: A thermosensitive chlorophyll deficient mutant was obtained from the progeny of tissue culture of a japonica rice cultivar Asominori. The mutant was characterized by albino leaves before 3leaf stage under low temperature (23℃), while showing normal phenotype under high temperature(30℃). The chlorophyll contents of the mutant were significantly lower than those of wildtype before 3leaf stage under low temperature, which is due to altered chloroplast development under low temperature. Genetic analysis showed that the mutation was controlled by a single recessive gene, which is tentatively designed as cde2(chlorophyll deficient 2). Furthermore, using an F2 population derived from cde2/Peiai 64 and polymorphic markers between cde2 and Peiai 64, the cde2 was finally localized on the centromeric region of chromosome 1 and positioned between SSR markers RM11041 and Indel1, within the physical distance about 365.6 kb. In addition, the expression level of genes associated with chlorophyll biosynthesis, photosynthesis and plastidial transcription/translation apparatus was measured and the results shows that the cde2 mutation increased the transcript level of chlorophyll biosynthesis genes and the platidial transcription/translation apparatus related genes, but reduced the expression level of photosynthesis genes, indicating that CDE2 may involve in the chlorophyll biosynthesis and chloroplast development.

Key words: rice, chlorophyll deficient, thermosensitive mutant, gene mapping

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