旱稻孢囊线虫在广西的发生及其rDNAITS异质性分析

doi:10.3969/j.issn.1001-7216.2014.01.011

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (1): 78-84.DOI: 10.3969/j.issn.1001-7216.2014.01.011

旱稻孢囊线虫在广西的发生及其rDNAITS异质性分析

卓侃¹,宋汉达¹ ,王宏洪¹,陶冶¹ ,张洪玲¹,陆秀红²,黄金玲²,刘志明² ,廖金铃^1,*

¹华南农业大学资源环境学院植物线虫研究室，广州 510642；²广西农业科学院植物保护研究所，南宁 530007；

收稿日期:2013-07-30 修回日期:2013-10-01 出版日期:2014-01-10 发布日期:2014-01-10
通讯作者: 廖金铃1,*
基金资助:
公益性行业（农业）科研专项经费资助项目（200903040）。

Occurence of Heterodera elachista in Guangxi Region and Its Intraspecies Heterogeneity in rDNAITS Region

ZHUO Kan¹, SONG Handa¹, WANG Honghong¹, TAO Ye¹, ZHANG Hongling¹, LU Xiuhong², HUANG Jinling², LIU Zhiming², LIAO Jinling^1,*

¹Laboratory of Plant Nematology, College of Natural Resource and Environment, South China Agricultural University, Guangzhou 510642, China; ²Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;

Received:2013-07-30 Revised:2013-10-01 Online:2014-01-10 Published:2014-01-10
Contact: LIAO Jinling1,*

摘要/Abstract

摘要： 2011年，从广西龙胜梯田的水稻根系和稻田土壤中分离到一种孢囊线虫。该线虫形态特征与已报道的旱稻孢囊线虫（Heterodera elachista）相符，其rDNAITS序列与GenBank中旱稻孢囊线虫的rDNAITS序列相似性为97.7%~99.3%。rDNAITS分子发育树表明该线虫与旱稻孢囊线虫以100%的置信度及较小的遗传距离形成一个单系，据此将该线虫鉴定为旱稻孢囊线虫。进一步对本研究获得的10条旱稻孢囊线虫rDNAITS区序列及Tanha Maafi等报道的1条旱稻孢囊线虫rDNAITS序列（AF498391）的酶切位点进行分析，结果发现AluⅠ、AvaⅠ、MvaⅠ和RsaⅠ 4个内切酶的酶切位点在11条序列中完全一致，而Bsh1236Ⅰ、BsuRⅠ和CfoⅠ 3种酶的酶切位点存在变异，其中Bsh1236Ⅰ和BsuRⅠ有2种变异型，CfoⅠ则有3种变异型，表明旱稻孢囊线虫的rDNAITS序列存在DNA异质性。

关键词: 旱稻孢囊线虫, 水稻, 鉴定, rDNAITS, 异质性

Abstract: In 2011, a cyst nematode was isolated from rice roots and soils in Longsheng Terrace of Guangxi Region, China. Morphological characteristics of the cyst nematode were consistent with previous descriptions of Heterodera elachista. The rDNAITS sequences of the cyst nematode and H. elachista in GenBank shared 97.7%-99.3% identities. The phylogenetic tree based on rDNAITS showed that the cyst nematode was in the same monophyletic clade as H. elachista with 100% support and short branch differences. The results indicated the cyst nematode was Heterodera elachista. The restriction enzyme cutting sites of ten rDNAITS sequences of H. elachista amplified and the one reported by Tanha Maafi (GenBank accession number: AF498391) were analyzed. The restriction enzyme cutting sites of AluⅠ, AvaⅠ, MvaⅠ and RsaⅠ in these 11 sequences were identical, however there were 2 different patterns after Bsh1236Ⅰ and BsuRⅠ digestion, respectively, and 3 different patterns after CfoⅠ digestion. The results show intraspecies heterogeneity in rDNAITS region exists in H. elachista.

Key words: Heterodera elachista, rice, identification, rDNAITS, heterogeneity

中图分类号:

卓侃1,宋汉达1 ,王宏洪1 ,陶冶1 ,张洪玲1,陆秀红2,黄金玲2,刘志明2 ,廖金铃1,*. 旱稻孢囊线虫在广西的发生及其rDNAITS异质性分析[J]. 中国水稻科学, 2014, 28(1): 78-84.

ZHUO Kan1, SONG Handa1, WANG Honghong1, TAO Ye1, ZHANG Hongling1, LU Xiuhong2, HUANG Jinling2, LIU Zhiming2, LIAO Jinling1,*. Occurence of Heterodera elachista in Guangxi Region and Its Intraspecies Heterogeneity in rDNAITS Region[J]. Chinese Journal of Rice Science, 2014, 28(1): 78-84.

参考文献

\[1\]McGawley E C, Overstreet C. Nematode parasites of rice and other cereals // Monograph on Plantnematode Interactions. Madison (WI): American Society of Agronomy, 1998: 455486.

\[2\]Fortuner R, Merny G. Rootparasitic nematodes of rice. Revue Nématol, 1979, 2 (1): 79102.

\[3\]Bridge J, Plowright R A, Peng D L. Nematode parasites of rice // Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. Wallingford, UK: CAB International, 2005: 87130.

\[4\]Subbotin S A, MundoOcampo M, Baldwin J G. Systematics of cyst nematodes (Nematoda: Heteroderinae) // Nematology Monographs and Perspectives 8B. Leiden, the Netherlands: Brill, 2010: 3549.

\[5\]李怡珍，金殿文，陈纯. 水稻孢囊线虫鉴定初报. 植物检疫，1985(1)： 5457.

\[6\]Ding Z, Namphueng J, He X F, et al. First report of the cyst nematode (Heterodera elachista) on rice in Hunan Province, China. Plant Dis, 2012: 96(1): 151.

\[7\]Shimizu K. Influence of the rice cyst nematode on the yield of paddy rice // Annual Meeting of the Japanese Society of Applied Entomology and Zoology. Fuchu, Tokyo, 1971: 32.

\[8\]Ohshima Y. Heterodera elachista n. sp., an upland rice cyst nematode from Japan. Jpn J Nematol, 1974, 4: 5156.

\[9\]Subbotin S A, MundoOcampo M, Baldwin J G. Systematics of cyst nematodes (Nematoda: Heteroderinae) // Nematology Monographs and Perspectives 8A. Leiden, the Netherlands: Brill, 2010: 291294.

\[10\]MundoOcampo M, Troccoli A, Subbotin SA, et al. Synonymy of Afenestrata with Heterodera supported by phylogenetics with molecular and morphological characterisation of H. koreana comb. n. and H. orientalis comb. n. (Tylenchida: Heteroderidae). Nematology, 2008, 10: 611632.

\[11\]Subbotin S A, Waeyenberge L, Moens M. Identification of cyst forming nematodes of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNARFLPs. Nematology, 2000, 2: 153164.

\[12\]王宏洪，卓侃，张洪玲，等. 孢囊线虫中国新纪录种——朝鲜孢囊线虫（Heterodera koreana）. 植物病理学报，2012，42（5）：551555.

\[13\] Zhuo K, Wang H H, Ye W, et al.Heterodera hainanensis n. sp. (Nematoda: Heteroderinae) from bamboo in Hainan Province, China-a new cyst nematode in the Afenestrata group. Nematology, 2013, 15: 303314.

\[14\]Tanha Maafi Z, Subbotin S A, Moens M. Molecular identification of cystforming nematodes (Heteroderidae) from Iran and a phylogeny based on ITSrDNA sequences. Nematology, 2003, 5: 99111.

\[15\]Tanha Maafi Z, Sturhan D, Kheiri A, et al. Morphology of some cystforming nematodes from Iran. Russ J Nematol, 2004, 12: 5977.

\[16\]Nguyen C N, Sturhan D, Subbotin S A. Studies on the occurrence and diversity of Heteroderidae and Meloidogynidae (Nematoda: Tylenchida) in natural forests of Vietnam. Russ J Nematol, 2011, 19: 159172.

\[17\] Wang H H, Zhuo K, Ye W, et al.Heterodera fengi n. sp. (Nematoda: Heteroderinae) from bamboo in Guangdong Province, China-a new cyst nematode in the Cyperi group. Zootaxa, 2013, 3652 (1): 179192.

\[18\] Burgermeister W, Braasch H, Metge K, et al. ITSRFLP analysis, an efficient tool for differentiation of Bursaphelenchus species. Nematology, 2009, 11: 649668.

\[19\] Shimizu K. Influence of the upland rice cyst nematode,Heterodera elachista, on the yield of the uplandcultured paddy rice. Jpn J Nematol, 1976, 6: 16.

\[20\] 贺沛成，洪宏，伍敏敏，等. 旱稻孢囊线虫（Heterodera elachista）孵化特性研究. 植物保护， 2012， 37（6）： 101103.

\[21\]丁中， Janthathang N，何旭峰，等. 旱稻孢囊线虫生活史及侵染特性. 中国水稻科学， 2012， 26（6）： 746750.

[1]	任志奇, 薛可欣, 董铮, 李小湘, 黎用朝, 郭玉静, 刘文强, 郭梁, 盛新年, 刘之熙, 潘孝武. 水稻外卷叶突变体ocl1的鉴定及基因定位[J]. 中国水稻科学, 2023, 37(4): 337-346.
[2]	肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A/bar*基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358.
[3]	李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367.
[4]	汪胜勇, 陈宇航, 陈会丽, 黄钰杰, 张啸天, 丁双成, 王宏伟. 水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响[J]. 中国水稻科学, 2023, 37(4): 368-378.
[5]	董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404.
[6]	韩聪, 何禹畅, 吴丽娟, 郏丽丽, 王磊, 鄂志国. 水稻碱性亮氨酸拉链(bZIP)蛋白家族功能研究进展[J]. 中国水稻科学, 2023, 37(4): 436-448.
[7]	沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232.
[8]	段敏, 谢留杰, 高秀莹, 唐海娟, 黄善军, 潘晓飚. 利用CRISPR/Cas9技术创制广亲和水稻温敏雄性不育系[J]. 中国水稻科学, 2023, 37(3): 233-243.
[9]	程玲, 黄福钢, 邱一埔, 王心怡, 舒宛, 邱永福, 李发活. 籼稻材料570011抗褐飞虱基因的遗传分析及鉴定[J]. 中国水稻科学, 2023, 37(3): 244-252.
[10]	王文婷, 马佳颖, 李光彦, 符卫蒙, 李沪波, 林洁, 陈婷婷, 奉保华, 陶龙兴, 符冠富, 秦叶波. 高温下不同施肥量对水稻产量品质形成的影响及其与能量代谢的关系分析[J]. 中国水稻科学, 2023, 37(3): 253-264.
[11]	刘嫒桦, 李小坤. 不同肥料施用与稻米品质关系的整合分析[J]. 中国水稻科学, 2023, 37(3): 276-284.
[12]	杨晓龙, 王彪, 汪本福, 张枝盛, 张作林, 杨蓝天, 程建平, 李阳. 不同水分管理方式对旱直播水稻产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 285-294.
[13]	魏晓东, 宋雪梅, 赵凌, 赵庆勇, 陈涛, 路凯, 朱镇, 黄胜东, 王才林, 张亚东. 硅锌肥及其施用方式对南粳46产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 295-306.
[14]	林聃, 江敏, 苗波, 郭萌, 石春林. 水稻高温热害模型研究及其在福建省的应用[J]. 中国水稻科学, 2023, 37(3): 307-320.
[15]	郑承梅, 孙金秋, 刘梦杰, 杨永杰, 陆永良, 郭怡卿, 唐伟. 水稻田糠稷种子萌发和出苗特性及化学防除药剂筛选[J]. 中国水稻科学, 2023, 37(3): 321-328.

旱稻孢囊线虫在广西的发生及其rDNAITS异质性分析

Occurence of Heterodera elachista in Guangxi Region and Its Intraspecies Heterogeneity in rDNAITS Region

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics