中国水稻科学

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (5): 529-534.DOI: 10.3969/j.issn.1001-7216.2013.05.010

• 实验技术 • 上一篇    下一篇

利用四引物扩增受阻突变体系PCR技术检测水稻低直链淀粉含量基因Wxmq

陈涛,骆名瑞,张亚东,朱镇,赵凌,赵庆勇,周丽慧,姚姝,于新,王才林*   

  1. 江苏省农业科学院 粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心， 南京 210014；
  • 收稿日期:2012-10-23 修回日期:2012-12-06 出版日期:2013-09-10 发布日期:2013-09-10
  • 通讯作者: 王才林*
  • 基金资助:

    江苏省农业科技自主创新基金资助项目\[CX(11)4021\]；江苏省科技支撑计划资助项目（BE2011302）； 农业部超级稻育种与示范项目； 现代农业产业技术体系建设专项资金资助项目（CARS0147）。

Detection of Wxmq Gene for Lowamylose Content by Tetraprimer  Amplification Refractory Mutation System PCR in Rice

CHEN Tao， LUO Mingrui， ZHANG Yadong， ZHU Zhen， ZHAO Ling， ZHAO Qingyong， ZHOU Lihui， YAO Shu， YU Xin， WANG Cailin*   

  1. Institute of Food Crops， Jiangsu Academy of Agricultural Sciences， Jiangsu High Quality Rice R&D Center，   Nanjing Branch of China National Center for Rice Improvement， Nanjing 210014, China；
  • Received:2012-10-23 Revised:2012-12-06 Online:2013-09-10 Published:2013-09-10
  • Contact: WANG Cailin*

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摘要： 根据Wxmq基因存在的单核苷酸变异，利用四引物扩增受阻突变体系PCR（Tetraprimer ARMSPCR）的方法设计特异引物，对12个水稻品种（或品系）以及武育粳3号（不含Wxmq基因）/关东194（含Wxmq基因）的F2分离群体进行扩增，依据其PCR产物的带型，可以准确区分出Wxmq基因纯合、非Wxmq基因纯合以及杂合基因型三种类型，其电泳结果与成熟后对胚乳外观特性鉴定完全一致。因此，作为一种简单、低成本的实用技术，四引物ARMSPCR可以广泛用于Wxmq基因水稻的资源鉴定和分子标记辅助育种。

关键词: 水稻, 低直链淀粉含量, Wxmq基因, 四引物扩增受阻突变体系PCR

Abstract: To improve the efficiency of selection for Wxmq gene in breeding, we designed specific primers by tetraprimer Amplification Refractory Mutation System PCR (ARMSPCR) method, according to the single nucleotide mutation in Wxmq gene to analyze 12 rice varieties (or lines) and the F2 segregation population derived from the cross of lowamylose content variety Kanto 194 (with Wxmq gene) and normal variety Wuyujing 3(without Wxmq gene). The results of PCR detection indicated that three genotypes including the homozygous genotype with Wxmq, the homozygous genotype without Wxmq and the heterozygous genotype could be clearly distinguished according to band types, and it was completely consistent with the result of phenotypic analysis. Therefore, as a simple and lowcost technique，the tetraprimer ARMSPCR could be widely used to identify rice resources with Wxmq gene and applied in molecular markerassisted breeding.

Key words: rice, lowamylose content, Wxmq gene, tetraprimer ARMSPCR

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