水稻单片段代换系芽期和苗期耐冷性分析及耐冷性QTL鉴定

doi:10.3969/j.issn.1001-7216.2013.04.007

摘要/Abstract

摘要： 对籼型两系骨干亲本9311（受体）和粳稻日本晴（供体）构建的单片段代换系群体，在芽期和苗期分别进行低温处理，以芽期处理后的恢复成活率和苗期卷叶程度作为耐冷性的评价指标进行耐冷性评价。结果表明，日本晴在芽期和苗期耐冷性极显著强于9311，不同代换系芽期和苗期耐冷性也存在极显著差异，芽期和苗期耐冷性较强的代换系X724、X732、X733较好地保持了9311的农艺性状，其产量等性状的一般配合力优于9311；鉴定出18个芽期耐冷性QTL和5个苗期耐冷性QTL，分布于12条染色体上的20个代换片段中，第12染色体的RM1261-RM519和RM17区域同时检测出芽期和苗期耐冷性QTL；芽期耐冷性QTL qCTP9、qCTP11.2、qCTP12.1和苗期耐冷性QTL qCTS1.1、qCTS1.2具有较大加性效应，对应代换系耐冷性较强。

关键词: 水稻, 单片段代换系, 芽期, 苗期, 耐冷性, 数量性状基因座

Abstract: The cold tolerance at plumule stage (CTP) and seedling stage (CTS) were respectively evaluated at 4℃ and 13℃/7℃ (day/night) using a set of single segment substitution lines (SSSLs) derived from indica recipient (9311) and japonica donor (Nipponbare). The survival seedling rate and the normal leaf rate (NLR) were used as the estimation index for CTP and CTS, respectively. The results showed that the CTP and CTS of Nipponbare were extremely stronger than those of 9311, and the difference of CTP and CTS were extremely significant among SSSLs. The SSSLs (X724, X732 and X733) with strong CTP and CTS mostly kept the agronomic traits of 9311. The General Combing Ability (GCA) of these three SSSLs, such as yield GCA, were much better than that of 9311. A total of 18 quantitative trait loci (QTLs) for CTP, 5 QTLs for CTS were identified among 20 introgression segments distributed on 12 chromosomes. Among these QTLs, the CTP QTLs (qCTP9, qCTP11.2 and qCTP12.1), and the CTS QTLs (qCTS1.1 and qCTS1.2) showed more additive effect, and the corresponding SSSLs of those QTLs displayed stronger CTP and CTS, respectively.

Key words: rice, single segment substitution lines, plumule stage, seedling stage, cold tolerance, QTL

中图分类号:

周勇1,2 ,朱孝波1 ,袁华1 ,郑英2 ,钦鹏1 ,魏应海1，2 ,王玉平1 ,黄世君2 ,李仕贵1,*. 水稻单片段代换系芽期和苗期耐冷性分析及耐冷性QTL鉴定[J]. 中国水稻科学, 2013, 27(4): 381-388.

ZHOU Yong1，2 , ZHU Xiaobo１， YUAN Hua1， ZHENG Ying2， QIN Peng１， WEI Yinghai1，2， WANG Yuping1， HUANG Shijun2， LI Shigui1，*. Characterization of Cold Tolerance and Identification of Cold Tolerance QTLs for Rice Single Segment Substitution Lines at Plumule and Seedling Stages[J]. Chinese Journal of Rice Science, 2013, 27(4): 381-388.

参考文献

\[1\]Lou Q J, Chen L, Sun Z X, et al. A major QTL associated with cold tolerance at seedling stage in rice(Oryza sativa L.). Euphytica, 2007, 158: 8794.

\[2\]Sthapit B R, Witcombe J R. Inheritance of tolerance to chilling stress in rice during germination and plumule greening. Crop Sci, 1998, 38: 660665.

\[3\]潘英华, 郑薇薇, 李金杰, 等. 水稻耐冷性鉴定及定位研究概况. 中国农学通报, 2010, 26(17): 5459.

\[4\]Fujino K, Sekiguchi H, Sato T, et al. Mapping of quantitative trait loci controlling lowtemperature germinability in rice(Oryza sativa L.). Theor Appl Genet, 2004, 108: 794799.

\[5\]Andaya V C, Mackill D J. Mapping of QTLs associated with cold tolerance during the vegetative stage in rice. J Exp Bot, 2003, 54: 25792585.

\[6\]Koseki M, Kitazawa N, Yonebayashi S, et al. Identification and fine mapping of a major quantitative trait locus origination from wild rice, controlling cold tolerance at the seedling stage. Mol Genet Genom, 2010, 284: 4554.

\[7\]Zhou L, Zeng Y W, Hu G L, et al. Characterization and identification of cold tolerant nearisogenic lines in rice. Breeding Sci, 2012, 62: 196201.

\[8\]Baruah A R, Oka N I, Adachi M, et al. Cold tolerance at the early growth stage in wild and cultivated rice. Euphytica, 2009, 165: 459470.

\[9\]Jin R Z. Genetics and evaluation of cold tolerance of rice in Japan. Rice Rev Abstr, 1990, 9: 15.

\[10\]Cruz R P D, Milach S C K, Federizzi L C. Inheritance of rice cold tolerance at the germination stage. Genet Mol Biol, 2006, 29: 314320.

\[11\]简水溶, 万勇, 罗向东, 等. 东乡野生稻苗期耐冷性的遗传分析. 植物学报, 2011, 46(1): 2127.

\[12\]Zhao X C, Dolferus R, Drvey N. Precision breeding of cold tolerant rice. IREC Farmers’ Newsl, 2007, 177: 1213.

\[13\]Shahi B B, Khuxh G S. Genetic analysis of cold tolerance in rice. Rice Genet, 1986, 1: 429435.

\[14\]Zhang Z H, Su L, Li W, et al. A major QTL conferring cold tolerance at the early seedling stage using recombinant inbred lines of rice (Oryza sativa L. ). Plant Sci, 2005, 168: 527534.

\[15\]陈玮, 李炜. 水稻RIL群体芽期耐冷性基因的分子标记定位. 武汉植物学研究, 2005, 23(2): 116120.

\[16\]严长杰, 李欣, 程祝宽, 等. 利用分子标记定位水稻芽期耐冷性基因. 中国水稻科学, 1999, 13(3): 134138.

\[17\]乔永利, 韩龙植, 安永平, 等. 水稻芽期耐冷性QTL的分子定位. 中国农业科学, 2005, 38: 217221.

\[18\]杨杰, 仲维功, 王军, 等. 水稻芽期耐冷性的QTL分析. 基因组学与应用生物学, 2009, 28(1): 4650.

\[19\]巩迎军, 阮雯君, 荀星, 等. 水稻芽性状耐冷性的QTL分析. 分子植物育种, 2009, 7(2): 273278.

\[20\]Lin J, Zhu W Y, Zhang Y D, et al. Detection of QTL for cold tolerance at bud bursting stage using chromosome segment substitution lines in rice (Oryza sativa L.). Rice Sci, 2011, 18: 7174.

\[21\]Suh J P, Lee C K, Lee J H, et al. Identification of quantitative trait loci for seedling cold tolerance using RILs derived from a cross between japonica and tropical japonica rice cultivars. Euphytica, 2012, 184: 101108.

\[22\]钱前, 曾大力, 何平, 等. 水稻籼粳交DH群体苗期的耐冷性QTLs分析. 科学通报, 1999, 44(22): 24022407.

\[23\]Misawa S, Mori N, Takumi S, et al. Mapping of QTLs for lowtemperature response in seedlings of rice (Oryza sativa L.). Cereal Res Commun, 2000, 28: 3340.

\[24\]Han L Z, Qiao Y L, Cao G L, et al. QTLs analysis of cold tolerance during early growth period for rice. Rice Sci, 2004, 11: 245250.

\[25\]夏瑞祥, 肖宁, 洪义欢, 等. 东乡野生稻苗期耐冷性的QTL定位. 中国农业科学, 2010, 43(3): 443451.

\[26\]Zhu W Y, Lin J, Yang D W, et al. Development of chromosome segment substitution lines derived from backcross between two sequenced rice cultivars, indica recipient 9311 and japonica donor Nipponbare. Plant Mol Biol Rep, 2009, 27: 126131.

\[27\]Xi Z Y, He F H, Zeng R Z, et al. Development of a wide population of chromosome singlesegment substitution lines in the genetic background of an elite cultivar of rice (Oryza sativa L.). Genome, 2006, 49: 476484.

\[28\]杨川航, 王玉平, 涂斌, 等. 利用籼粳交RIL群体对水稻耐寒性及再生力的QTL分析. 中国水稻科学, 2012, 26（6）: 741745.

\[29\]徐云碧, 申宗坦. 水稻苗期耐冷性鉴定技术的研究. 浙江农业大学学报, 1990, 10(1): 2530.

\[30\]Eshed Y, Zamir D. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yieldassociated QTL. Genetics, 1995, 141: 11471162.

\[31\]Zhang Y S, Yang J Y, Shan Z L, et al. Substitution mapping of QTLs for blast resistance with SSSLs in rice (Oryza sativa L. ). Euphytica, 2012, 184(1): 141150.

\[32\]McCouch S R, CGSNL. Gene nomenclature system for rice. Rice, 2008, 1: 7284.

\[33\]Glaszmann J C. Isozymes and classification of Asian rice varieties. Theor Appl Genet, 1987, 74: 2130.

\[34\]Glaszmann J C, Kaw R N, Khush G S. Genetic divergence among cold tolerance rices (Oryza sativa L. ). Euphytica, 1990, 45: 95104.

\[35\]Kubo T, Yoshimura A. Complementary genes causing F2 sterility in japonica/indica cross of rice. Rice Genet Newsl, 1999, 16: 6870.

\[36\]Kubo T, Eguchi M, Yoshimura A. A new gene for F1 pollen sterility in japonica/indica cross of rice. Rice Genet Newsl, 2000, 17: 6364.

\[37\]Han L Z, Qiao Y L, Zhang S Y, et al. Identification of quantitative trait loci for cold response of seedling vigor traits in rice. J Genet Genom, 2007, 34(3): 239246.

\[38\]Xu L M, Zhou L, Zeng Y W, et al. Identification and mapping of quantitative trait loci for cold tolerance at the booting stage in a japonica rice nearisogenic line. Plant Sci, 2008, 174: 340347.

\[39\]Han L Z, Zhang Y Y, Qiao Y L, et al. Genetic and QTL analysis for lowtemperature vigor of germination in rice. Acta Gen Sin, 2006, 33(11): 9981006.

\[40\]Dai L Y, Lin X H, Ye C R, et al. Identification of quantitative trait loci controlling cold tolerance at the reproductive stage in Yunnan landrace of rice, Kunmingxiaobaigu. Breeding Sci, 2004, 54: 253258.

\[41\]Suh J P, Jeung J U, Lee J I, et al. Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in coldtolerant genotypes of rice (Oryza sativa L. ). Theor Appl Genet, 2010, 120: 985995.

\[42\]Andaya V C, Mackill D J. QTLs conferring cold tolerance at the booting stage of rice using ricombinant inbred lines from a japonica×indica cross. Theor Appl Genet, 2003, 106: 10841090.

\[43\]詹庆才, 朱克永, 陈祖武, 等. 利用分子标记进行水稻苗期耐冷性相关性状的QTLs研究. 杂交水稻, 2005, 20(1): 5055.

\[44\]Oh C S, Choi Y H, Lee S J, et al. Mapping of quantitative trait loci for cold tolerance in weedy rice. Breeding Sci, 2004, 54: 373380.

\[45\]Ranawake A L, Nakamura C. Cold tolerance of an inbred line population of rice (Oryza sativa L.) at different growth stages. Trop Agric Res & Ext, 2011, 14: 2533.

\[46\]Kaw R N, Khush G S. Combining ability for lowtemperature tolerance in rice//Proceedings of the International Rice Genetics Symposium. Los Banos, Philippines, 2008: 593612.

[1]	任志奇, 薛可欣, 董铮, 李小湘, 黎用朝, 郭玉静, 刘文强, 郭梁, 盛新年, 刘之熙, 潘孝武. 水稻外卷叶突变体ocl1的鉴定及基因定位[J]. 中国水稻科学, 2023, 37(4): 337-346.
[2]	肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A/bar*基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358.
[3]	李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367.
[4]	汪胜勇, 陈宇航, 陈会丽, 黄钰杰, 张啸天, 丁双成, 王宏伟. 水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响[J]. 中国水稻科学, 2023, 37(4): 368-378.
[5]	董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404.
[6]	韩聪, 何禹畅, 吴丽娟, 郏丽丽, 王磊, 鄂志国. 水稻碱性亮氨酸拉链(bZIP)蛋白家族功能研究进展[J]. 中国水稻科学, 2023, 37(4): 436-448.
[7]	沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232.
[8]	段敏, 谢留杰, 高秀莹, 唐海娟, 黄善军, 潘晓飚. 利用CRISPR/Cas9技术创制广亲和水稻温敏雄性不育系[J]. 中国水稻科学, 2023, 37(3): 233-243.
[9]	程玲, 黄福钢, 邱一埔, 王心怡, 舒宛, 邱永福, 李发活. 籼稻材料570011抗褐飞虱基因的遗传分析及鉴定[J]. 中国水稻科学, 2023, 37(3): 244-252.
[10]	王文婷, 马佳颖, 李光彦, 符卫蒙, 李沪波, 林洁, 陈婷婷, 奉保华, 陶龙兴, 符冠富, 秦叶波. 高温下不同施肥量对水稻产量品质形成的影响及其与能量代谢的关系分析[J]. 中国水稻科学, 2023, 37(3): 253-264.
[11]	刘嫒桦, 李小坤. 不同肥料施用与稻米品质关系的整合分析[J]. 中国水稻科学, 2023, 37(3): 276-284.
[12]	杨晓龙, 王彪, 汪本福, 张枝盛, 张作林, 杨蓝天, 程建平, 李阳. 不同水分管理方式对旱直播水稻产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 285-294.
[13]	魏晓东, 宋雪梅, 赵凌, 赵庆勇, 陈涛, 路凯, 朱镇, 黄胜东, 王才林, 张亚东. 硅锌肥及其施用方式对南粳46产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 295-306.
[14]	林聃, 江敏, 苗波, 郭萌, 石春林. 水稻高温热害模型研究及其在福建省的应用[J]. 中国水稻科学, 2023, 37(3): 307-320.
[15]	郑承梅, 孙金秋, 刘梦杰, 杨永杰, 陆永良, 郭怡卿, 唐伟. 水稻田糠稷种子萌发和出苗特性及化学防除药剂筛选[J]. 中国水稻科学, 2023, 37(3): 321-328.