中国水稻科学

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (4): 353-358.DOI: 10.3969/j.issn.1001-7216.2013.04.003

• 研究报告 • 上一篇    下一篇

DDF1的表达水平影响水稻的营养生长与生殖发育

刁志娟1,3 ,李生平1 ,段远霖1,2，* ,郑磊磊1 ,周元昌1,2 ,吴为人1,2，*   

  1. 1福建农林大学 作物遗传育种与综合利用教育部重点实验室, 福州 350002;2福建农林大学 福建省水稻分子标记辅助育种重点实验室, 福州 350002; 3福建农林大学 生命科学学院，福州 350002;
  • 收稿日期:2013-01-04 修回日期:2013-03-16 出版日期:2013-07-10 发布日期:2013-07-10
  • 通讯作者: 段远霖1,2，* ,吴为人1,2，*
  • 基金资助:

    国家自然科学基金资助项目（30970175, 30671122）；教育部博士点基金资助项目（20093515110003）；福建省自然科学基金重点资助项目（B0620001）。

Expression Level of  DDF1 Affects Vegetative Growth and Reproductive Development in Rice

DIAO Zhijuan 1,3 , LI Shengping 1 ,  DUAN Yuanlin 1,2,* , ZHENG Leilei 1 , ZHOU  Yuanchang 1,2 , WU Weiren 1,2,*   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agricultural and Forestry University, Fuzhou 350002, China; 2  Fujian Provincial Key Laboratory of MarkerAssisted Breeding of Rice, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3  College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
  • Received:2013-01-04 Revised:2013-03-16 Online:2013-07-10 Published:2013-07-10
  • Contact: DUAN Yuanlin1,2,*,WU Weiren1,2,*

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摘要： DDF1（Dwarf and Deformed Flower 1）是控制水稻生长发育的重要调节基因。为了进一步分析DDF1对水稻生长与发育的影响，我们构建了DDF1的抑制表达和增强表达载体并转化水稻品种中花15。结果显示，DDF1表达量下降的转基因水稻的表型类似于功能缺失突变体ddf11，表现为植株矮化、叶片细小，小穗中浆片同源转变为稃片，雄蕊不育且畸形，雌蕊可育；而DDF1增强表达的转基因植株营养生长正常，但大部分小穗发育异常，尤其是稃片和雄蕊数目明显增加。因此，通过调节该基因的表达量可以显著影响水稻的株型和育性。

关键词: 水稻, DDF1, 抑制表达, 过量表达

Abstract: DDF1 (Dwarf and Deformed Flower 1) is a very important gene regulating the growth and development in rice. In order to further analyze the influence of DDF1 on plant growth and development, we constructed the plasmids of inhibition and enhancement of DDF1expression, and introduced them into rice variety Zhonghua 15. The transgenic plants with reduced DDF1expression showed phenotypes similar to those of the lossoffunction mutant ddf11,including dwarf, narrower and smaller leaves, homeotic transformation of lodicules into glumes, and sterile and deformed stamens and normal carpels in spikelets. Whereas, the transgenic plants with enhanced DDF1expression showed normal vegetative growth but abnormal development in most spikelets, in particular the significantly increased numbers of glumes and stamens. Therefore, by regulating the expression level of DDF1 gene, the plant stature and fertility of rice can be obviously affected. 

Key words: rice, DDF1, inhibition expression, enhancement expression

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