抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲

doi:10.3969/j.issn.10017216.2013.03.001

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (3): 223-230.DOI: 10.3969/j.issn.10017216.2013.03.001

• 研究报告 • 下一篇

抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲

李磊^1,# ,薛芗^1,# ,左示敏¹,陈宗祥¹,张亚芳¹,李前前¹,朱俊凯²,马玉银³,潘学彪^1,* ,潘存红 ^2,*

¹扬州大学农学院, 江苏扬州 225009; ²江苏里下河地区农业科学研究所, 江苏扬州 225007; ³扬州职业大学生化工程系, 江苏扬州 225009;

收稿日期:2012-12-13 修回日期:2013-01-24 出版日期:2013-05-10 发布日期:2013-05-10
通讯作者: 潘学彪1,*,潘存红2,*
基金资助:
国家自然科学基金资助项目（31201180）；江苏省自然科学基金资助项目（BK2008223；BK2012690）。

Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice

LI Lei ^1,# , XUE Xiang ^1,# , ZUO Shimin ¹ , CHEN Zongxiang ¹ , ZHANG Yafang ¹, LI Qianqian¹ , ZHU Junkai ² , MA Yuyin ³ , PAN Xuebiao ^1,* , PAN Cunhong ^2,*

¹ Agricultural College, Yangzhou University, Yangzhou 225009, China; ² Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou 225007, China; ³ Department of Biochemical Engineering, Yangzhou Polytechnic College, Yangzhou 225009, China; #These authors contribute equally to this paper;

Received:2012-12-13 Revised:2013-01-24 Online:2013-05-10 Published:2013-05-10
Contact: PAN Xuebiao1,*, PAN Cunhong2,*

摘要/Abstract

摘要： AGO蛋白是RNA诱导沉默复合体的核心元件，在植物生长发育过程中发挥着重要作用。通过分别特异性地扩增OsAGO1a基因中第2外显子和第11~12外显子的片段，构建了两个干涉载体OsAGO1aI1E和OsAGO1aI2E。利用农杆菌介导法转化水稻品种日本晴，抑制OsAGO1a基因的表达，借此研究OsAGO1a的功能及其对水稻生长发育的影响。结果表明, 抑制OsAGO1a的表达导致叶片的近轴面卷曲而对其他主要农艺性状的影响不大。

关键词: 水稻, OsAGO1a, 极性发育, 卷叶, 农艺性状

Abstract: Argonaute (AGO) protein is the core effectors in RNAinduced silencing complexes (RISCs), playing an important role in plant development. The 2nd and the 11th-12th exon fragments of OsAGO1awere amplified independently to make two RNAi constructs: OsAGO1aI1E and OsAGO1aI2E. To clarify the function of OsAGO1a in rice development, the expression of OsAGO1a has been suppressed by introducing RNAi constructs to Nipponbare through Agrobacteriummediated transformation. The result showed that the reduced expression of OsAGO1a lead to adaxial leaf rolling without affecting main agronomic traits.

Key words: rice, OsAGO1a, polarity development, rolled leaf, agronomic traits

中图分类号:

李磊1,#,薛芗1,#,左示敏1,陈宗祥1,张亚芳1,李前前1,朱俊凯2,马玉银3,潘学彪1,*,潘存红2,*. 抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲[J]. 中国水稻科学, 2013, 27(3): 223-230.

LI Lei1,#, XUE Xiang1,#, ZUO Shimin1, CHEN Zongxiang1, ZHANG Yafang1, LI Qianqian1, ZHU Junkai2, MA Yuyin3, PAN Xuebiao1,*, PAN Cunhong2,* . Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice[J]. Chinese Journal of Rice Science, 2013, 27(3): 223-230.

参考文献

\[1\]Vaucheret H, Vazquez H, Crété P, et al. The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development. Genes Dev, 2004, 18:11871197.

\[2\]Song J J, Liu J, Tolia N H, et al. The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Nat Struct Biol, 2003, 10(12):10261032.

\[3\]Mi S J, Cai T, Hu Y G, et al. Sorting of small RNAs into Arabidopsis Argonaute complexes is directed by the 5’ terminal nucleotide. Cell, 2008, 133: 116127.

\[4\]Montgomery T A, Howell M D, Cuperus J T, et al. Specificity of ARGONAUTE7miR390 interaction and dual functionality in TAS3 transacting siRNA formation. Cell, 2008, 133:128141.

\[5\]Okamura K, Ishizuka A, Siomi H, et al. Distinct roles for Argonaute proteins in small RNAdirected RNA cleavage pathways. Genes Dev, 2004, 18:16551666.

\[6\]Liu J D, Carmell M A, Rivas F V, et al. Argonaute2 is the catalytic engine of mammalian RNAi. Science, 2004, 305:14371441.

\[7\]Meister G, Landthaler M, Patkaniowska A, et al. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol Cell, 2004, 15:185197.

\[8\]Baumberger N, Baulcombe D C. Arabidopsis ARGONAUTE1 is an RNA slicer that selectively recruits microRNAs and short interfering RNAs. Proc Natl Acad Sci, 2005, 102(33):1192811933.

\[9\]Sunkar R, Zhu J K. Novel and stressregulated microRNAs and other small RNAs from Arabidopsis. Plant Cell, 2004, 16: 20012019.

\[10\]Carrington J C, Ambros V. Role of microRNAs in plant and animal development. Science, 2003, 301: 336338.

\[11\]Kapoor M, Arora R, Lama T, et al. Genomewide identification, organization and phylogenetic analysis of Dicerlike, Argonaute and RNAdependent RNA polymerase gene families and their expression analysis during reproductive development and stress in rice. BMC Genom, 2008, 9: 451.

\[12\]Shi Z Y, Wang J, Wan X S, et al. Overexpression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erectleaf habit. Planta, 2007, 226: 99108.

\[13\]Wu L, Zhang Q, Zhou H, et al. Rice MicroRNA effector complexes and targets. Plant Cell Online, 2009, 21: 34213435.

\[14\]Thompson J D, Gibson T J, Plewniak F, et al. The CLUSTAL_X windows interface: Xexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25: 48764882.

\[15\]Tamura K, Dudley J, Nei M, et al. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4. 0. Mol Biol Evol, 2007, 24:15961599.

\[16\]Hiei Y, Ohta S, Komari T, et al. Efficient transformation of rice (Oryza sativa L. ) mediated by Agrobacterium and sequence analysis of the boundaries of the TDNA. Plant J, 1994, 6(2):271282.

\[17\]Xie Z, Kasschau K D, Carrington J C. Negative feedback regulation of DicerLike1 in Arabidopsis by microRNAguided mRNA degradation. Curr Biol, 2003, 13:784789.

\[18\]Zhong R, Ye Z H. Regulation of HDZIP III genes by microRNA 165. Plant Signal Behav, 2007, 2(5): 351353.

\[19\]Zhou G K, Kubo M, Zhong R, et al. Overexpression of miR165 affects apical meristem formation, organ polarity establishment and vascular development in Arabidopsis. Plant Cell Physiol, 2007, 48(3): 391404.

\[20\]Williams L, Grigg S P, Xie M, et al. Regulation of Arabidopsis shoot apical meristem and lateral organ formation by microRNA miR166g and its AtHDZIP target genes. Development, 2005, 132(16): 36573668.

\[21\]Emery J F, Floyd S K, Alvarez J, et al. Radial patterning of Arabidopsis shoots by class III HDZIP and KANADI genes. Curr Biol, 2003, 13(20):17681774.

\[22\]Mallory A C, Reinhart B J, JonesRhoades M W, et al. MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5’ region. Embo J, 2004, 23(16):33563364.

\[23\]Juarez M T, Kui J S, Thomas J, et al. MicroRNAmediated repression of rolled leaf1 specifies maize leaf polarity. Nature, 2004, 428: 8488.

\[24\]邵元健, 潘存红, 陈宗祥, 等. 水稻不完全隐性卷叶主基因rl(t)的精细定位. 科学通报, 2005, 50(19): 21072113.

\[25\]潘存红, 李磊, 陈宗祥, 等. 一个水稻卷叶基因rl(t)的精细定位. 中国水稻科学, 2011, 25: 455460.

\[26\]杨守仁, 张龙步, 王进民. 水稻理想株型育种的理论和方法初论. 中国农业科学, 1984, 15(3): 613.

\[27\]周开达, 马玉清, 刘太清, 等. 杂交水稻亚种间重穗型组合选育杂交水稻超高产育种的理论与实践. 四川农业大学学报, 1995, 13(4): 403407.

\[28\]袁隆平. 杂交水稻超高产育种. 杂交水稻, 1997, 12(6): 16.

[1]	任志奇, 薛可欣, 董铮, 李小湘, 黎用朝, 郭玉静, 刘文强, 郭梁, 盛新年, 刘之熙, 潘孝武. 水稻外卷叶突变体ocl1的鉴定及基因定位[J]. 中国水稻科学, 2023, 37(4): 337-346.
[2]	肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A/bar*基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358.
[3]	李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367.
[4]	汪胜勇, 陈宇航, 陈会丽, 黄钰杰, 张啸天, 丁双成, 王宏伟. 水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响[J]. 中国水稻科学, 2023, 37(4): 368-378.
[5]	董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404.
[6]	韩聪, 何禹畅, 吴丽娟, 郏丽丽, 王磊, 鄂志国. 水稻碱性亮氨酸拉链(bZIP)蛋白家族功能研究进展[J]. 中国水稻科学, 2023, 37(4): 436-448.
[7]	沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232.
[8]	段敏, 谢留杰, 高秀莹, 唐海娟, 黄善军, 潘晓飚. 利用CRISPR/Cas9技术创制广亲和水稻温敏雄性不育系[J]. 中国水稻科学, 2023, 37(3): 233-243.
[9]	程玲, 黄福钢, 邱一埔, 王心怡, 舒宛, 邱永福, 李发活. 籼稻材料570011抗褐飞虱基因的遗传分析及鉴定[J]. 中国水稻科学, 2023, 37(3): 244-252.
[10]	王文婷, 马佳颖, 李光彦, 符卫蒙, 李沪波, 林洁, 陈婷婷, 奉保华, 陶龙兴, 符冠富, 秦叶波. 高温下不同施肥量对水稻产量品质形成的影响及其与能量代谢的关系分析[J]. 中国水稻科学, 2023, 37(3): 253-264.
[11]	刘嫒桦, 李小坤. 不同肥料施用与稻米品质关系的整合分析[J]. 中国水稻科学, 2023, 37(3): 276-284.
[12]	杨晓龙, 王彪, 汪本福, 张枝盛, 张作林, 杨蓝天, 程建平, 李阳. 不同水分管理方式对旱直播水稻产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 285-294.
[13]	魏晓东, 宋雪梅, 赵凌, 赵庆勇, 陈涛, 路凯, 朱镇, 黄胜东, 王才林, 张亚东. 硅锌肥及其施用方式对南粳46产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 295-306.
[14]	林聃, 江敏, 苗波, 郭萌, 石春林. 水稻高温热害模型研究及其在福建省的应用[J]. 中国水稻科学, 2023, 37(3): 307-320.
[15]	郑承梅, 孙金秋, 刘梦杰, 杨永杰, 陆永良, 郭怡卿, 唐伟. 水稻田糠稷种子萌发和出苗特性及化学防除药剂筛选[J]. 中国水稻科学, 2023, 37(3): 321-328.

抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲

Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics