水稻单片段代换系对两种不育细胞质恢复性的遗传分析

doi:10.3969/j.issn.10017216.2013.02.006

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (2): 145-152.DOI: 10.3969/j.issn.10017216.2013.02.006

水稻单片段代换系对两种不育细胞质恢复性的遗传分析

蔡健^1,2 ,代资举²,朱海涛²,曾瑞珍² ,张泽民² ,王敉敉¹,马同富^1,* ,张桂权^2,*

¹阜阳师范学院生命科学学院，安徽阜阳 236041； ²华南农业大学植物分子育种重点实验室, 广东广州 510642;

收稿日期:2012-08-06 修回日期:2012-08-22 出版日期:2013-03-10 发布日期:2013-03-10
通讯作者: 马同富1,* ,张桂权2,*
基金资助:
国家自然科学基金重点资助项目(30830074)；安徽省教育厅高校自然科学基金资助项目(KJ2010B155)。

Genetic Analysis of Restoration of Chromosome Single Segment Substitution Line to two Different CMS Lines in Rice

CAI Jian ^1,2, DAI Ziju ², ZHU Haitao ², ZENG Ruizhen ², ZHANG Zemin ², WANG Mimi ¹, MA Tongfu ^1,* , ZHANG Guiquan ^2,*

¹ School of Life Sciences, Fuyang Teachers College, Fuyang 236041, China; ²State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources, South China Agricultural University, Guangzhou 510642, China;

Received:2012-08-06 Revised:2012-08-22 Online:2013-03-10 Published:2013-03-10
Contact: MA Tongfu1,*, ZHANG Guiquan2,*

摘要/Abstract

摘要： 由华南农业大学植物分子育种实验室选育的水稻单片段代换系S42对野败型（WA型）和夜公型（Y型）细胞质雄性不育系均具有较强的恢复性。以野败型不育系珍汕97A和Y型不育系Y华农A为母本，单片段代换系S42为父本进行杂交，采用分子标记辅助选择和连续回交的方法构建了两个BC3F2群体。利用与第1、10染色体上恢复基因Rf3和Rf4两侧紧密连锁的SSR标记，从这两个BC3F2群体中筛选携带有基因型Rf3Rf3/rf4rf4和rf3rf3/Rf4Rf4的单株，对这些单株进行花粉和小穗育性观察，并利用205个多态性SSR标记对这些单株进行遗传背景分析，结果表明： 1）在同一细胞核背景下（S42），WA型不育细胞质的可恢复性好于Y型不育细胞质，单片段代换系S42中的恢复基因Rf4的恢复力大于Rf3; 2）单片段代换系S42中的恢复基因对于珍汕97A和Y华农A表现出质量数量性状的遗传。在单片段代换系S42中，除了主效恢复基因Rf3和Rf4外，微效基因或者修饰基因也表现出对珍汕97A和Y华农A的育性恢复作用，而且效应较大; 3）在构建的两个BC3F2群体中，基因型Rf3Rf3/rf4rf4和rf3rf3/Rf4Rf4单株的遗传背景片段数平均为1.1，对应于恢复基因Rf3和Rf4座位的代换片段平均长度分别为14.5 cM 和17.4 cM。

关键词: 水稻, 单片段代换系, 野败型, Y型, 恢复基因

Abstract: A rice chromosome single segment substitution line(SSSL) S42, which is a strong restorer line for wild abortive cytoplasmic male sterility(WACMS) and YCMS, was identified at the Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University. The genetic mode of fertility restorer(Rf) genes and the genetic relationship between WACMS and YCMS systems were characterized through the production of backcross populations. The SSSL S42 was used to pollinate WACMS line of Zhenshan 97A and YCMS line of YHuanong A and generated two BC3F2 populations by using markerassisted selection and traditional backcrossing. Results were shown as the following: 1) In the genetic background of S42, WACMS was restored more easily than YCMS, and the effect of Rf4 appeared to be slightly larger than that of Rf3 for the two CMS systems; 2) Two pairs of dominant genes Rf3 and Rf4 governed pollen fertility restoration, and modifying or minor genes also involved in the inheritance of restorer ability in S42, which indicates that the genetic mode of Rf genes was a qualitativequantitative character for WACMS and YCMS systems; 3) When 205 SSR markers were used to analyze the genetic background and average length substituted chromosome segments(SCS) of the two BC3F2 individuals, the number of SCS carried by BC3F2 individuals with the genotypes of Rf3Rf3/rf4rf4 or rf3rf3/Rf4Rf4, was 1.1, while the average lengths of the SCS, corresponding to Rf3 and Rf4 loci, were 14.5 cM and 17.4 cM, respectively.

Key words: rice, single segment substitution lines, wild abortive, Y type, restorer gene

中图分类号:

蔡健1,2 ,代资举2 ,朱海涛2 ,曾瑞珍2 ,张泽民2 ,王敉敉1 ,马同富1,* ,张桂权2,*. 水稻单片段代换系对两种不育细胞质恢复性的遗传分析[J]. 中国水稻科学, 2013, 27(2): 145-152.

CAI Jian1,2, DAI Ziju2, ZHU Haitao2, ZENG Ruizhen2, ZHANG Zemin2, WANG Mimi1, MA Tongfu1,*, ZHANG Guiquan2,*. Genetic Analysis of Restoration of Chromosome Single Segment Substitution Line to two Different CMS Lines in Rice[J]. Chinese Journal of Rice Science, 2013, 27(2): 145-152.

参考文献

\[1\]高明尉. 野败型杂交籼稻基因型的初步分析. 遗传学报, 198l, 8(1): 6674.

\[2\]杨仁崔, 卢浩然. 水稻恢复系IR24恢复基因的初步分析. 作物学报, 1984, 10(2): 8186.

\[3\]张桂权, 卢永根. 水稻质核互作孢子体雄性不育性的基因分析. 作物学报, 1987, 13(1): 2328.

\[4\]Zhang G, Lu Y, Huang N. Molecular analysis of introgressed chromosomal segments in a set of nearisogenic lines of rice for fertility restoring genes. Rice Genet Newsl, 1994, 11: 147149.

\[5\]Zhang G, Bharaj T S, Virmani S S, et al. Mapping of the Rf3 nuclear fertilityrestoring gene for WA cytoplasmic male sterility in rice using RAPD and RFLP markers.Theor Appl Genet, 1997, 94: 2733.

\[6\]Yao F Y, Xu C G, Yu S B, et al. Mapping and genetic analysis of two fertility restorer loci in the wild abortive cytoplasmic male sterility system of rice(Oryza sativa L.). Euphytica, 1997, 98:183187.

\[7\]Tan X L, Vanavichit S, Amornsilpa S, et al. Genetic analysis of rice CMSWA fertility restoration based on QTL mapping. Theor Appl Genet, 1998, 96: 994999.

\[8\]庄杰云, 樊叶杨, 吴建利, 等．水稻CMSWA育性恢复基因的定位．遗传学报, 2001, 28（2）： 129134.

\[9\]张群宇, 刘耀光, 张桂权, 等. 野败型水稻细胞质雄性不育恢复基因Rf4的分子标记定位. 遗传学报, 2002, 29(11): 10011004.

\[10\]Sattari M, Kathiresan A, Glenn B, et al. Comparative genetic analysis and molecular mapping of fertility restoration genes for WA,Dissi, and Gambiaca cytoplasmic male sterility systems in rice. Euphytica, 2008, 160: 305315.

\[11\]Sheeba N K, Viraktamath B C, Sivaramakrishnan S, et al. Validation of molecular markers linked to fertility restorer gene(s) for WACMS lines of rice. Euphytica, 2009, 167: 217227.

\[12\]Ngangkham U, Parida S K, De S, et al. Genic markers for wild abortive(WA) cytoplasm based male sterility and its fertility restoration in rice.Mol Breeding, 2010, 26: 275292.

\[13\]蔡善信. 华南晚籼夜公细胞质(Y型) 雄性不育系的选育. 中国水稻科学, 2002, 16(2): 185188.

\[14\]Zhang G Q, Zeng R Z, Zhang Z M, et al. The construction of a library of single segment substitution lines in rice(Oryza sativa L.). Rice Genet Newsl, 2004, 21: 8587.

\[15\]Xi Z Y, He F H,Zeng R Z, et al. Development of a wide population of chromosome singlesegment substitution lines in the genetic backgroundof an elite cultivar of rice(Oryza sativa L.). Genome, 2006, 49: 476484.

\[16\]廖秋平. 水稻(Oryza sativa L.) 细胞质雄性不育恢复性的等位基因分化\[硕士学位论文\]. 广州: 华南农业大学, 2008.

\[17\]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8(19): 43214325.

\[18\]Li W T, Zeng R Z, Zhang Z M, et al. Mapping of Sb locus for F1 pollen steril ity in cultivated rice using PCR based markers. Acta Bot Sin, 2002, 44(4): 463467.

\[19\]McCouch R, Teytelman L, Xu Y B, et al. Development and mapping of 2240 new SSR markers for rice(Oryza sativa L.). DNA Res, 2002, 9: 199207.

\[20\]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism(SSPL) in rice(Oryza sativa L.). Mol Gen Genet, 1996, 252: 597607.

\[21\]Singh A K, Mahapatra T, Prabhu K V, et al. Application of molecular markers in rice breeding: progress at IARI//Advances in Markerassisted Selection Workshop. Trainee’s manual, Handouts and references, 2005.

\[22\]张桂权, 卢永根. 栽培稻(Oryza sativa L.) 杂种不育性的遗传研究: I.等位基因 F1不育系杂种不育性的双列分析. 中国水稻科学, 1989, 3(3): 97101.

\[23\]Young N D, Tanksley S D. Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet, 1989, 77: 95101.

\[24\]黄朝锋. 水稻 PSM 标记的发展及抗虫基因的分子定位\[学位论文\]. 广州: 华南农业大学, 2003.

\[25\]Kubo T, Aida Y, Nakamura K, et al. Reciprocal chromosome segment substitution series derived from japonica and indica cross of rice(Oryza sativa L.). Breed Sci, 2002, 52: 319325.

\[26\]Ebitani T, Takeuchi Y, Nonoue Y, et al. Construction and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of indica rice cultivar ‘Kasalath’ in a genetic background of japonica elite cultivar ‘Koshihikari’. Breed Sci, 2005, 55: 6573.

\[27\]张丽霞, 刘丕庆, 刘学义, 等. 染色体单片段代换系的构建及应用于QTL精细定位. 分子植物育种, 2(3): 743746.

\[28\]刘冠明, 李文涛, 曾瑞珍, 等. 水稻亚种间单片段代换系的建立. 中国水稻科学, 2003, 17(3): 201204.

\[29\]何风华, 席章营, 曾瑞珍, 等. 利用高代回交和分子标记辅助选择建立水稻单片段代换系. 遗传学报, 2005, 32(8): 825831.

\[30\]He F H, Xi Z Y, Talukdar A, et al. Identification of QTLs for plant height and its components by using single segment substitution lines in rice(Oryza sativa L.). Rice Sci, 2005, 12(3): 151156.

\[31\]李文涛, 曾瑞珍, 张泽民, 等. F1花粉不育性近等基因系导入片段的分析. 中国水稻科学, 2003, 17(2): 9599.

\[32\]Bharaj T S, Virmani S S, Khush G S. Chromosomal location of fertility restoring genes for wild abortive cytoplasmic male sterility using primarytrisomics in rice. Euphytica, 1995, 83: 169173.

\[33\]徐才国, 唐为江, 邢永忠. 水稻优良恢复系明恢63的两个恢复基因恢复力的单独评价. 分子植物育种, 2003, 1(4): 497501.

\[34\]滕利生, 申宗坦. 水稻胞质不育的恢复基因分析. 作物学报, 1996, 22(2): 142146.

\[35\] 黄青阳, 何予卿, 凌杏元, 等. 3种水稻细胞质雄性不育系育性遗传的比较. 中国农业科学, 2000, 33(3): 813.

\[36\]Tao D Y, Xu P, Li J, et al. Inheritance and mapping of male sterility restoration gene in upland japonica restorer lines.Euphytica, 2004, 138: 247254.

[1]	任志奇, 薛可欣, 董铮, 李小湘, 黎用朝, 郭玉静, 刘文强, 郭梁, 盛新年, 刘之熙, 潘孝武. 水稻外卷叶突变体ocl1的鉴定及基因定位[J]. 中国水稻科学, 2023, 37(4): 337-346.
[2]	肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A/bar*基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358.
[3]	李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367.
[4]	汪胜勇, 陈宇航, 陈会丽, 黄钰杰, 张啸天, 丁双成, 王宏伟. 水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响[J]. 中国水稻科学, 2023, 37(4): 368-378.
[5]	董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404.
[6]	韩聪, 何禹畅, 吴丽娟, 郏丽丽, 王磊, 鄂志国. 水稻碱性亮氨酸拉链(bZIP)蛋白家族功能研究进展[J]. 中国水稻科学, 2023, 37(4): 436-448.
[7]	沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232.
[8]	段敏, 谢留杰, 高秀莹, 唐海娟, 黄善军, 潘晓飚. 利用CRISPR/Cas9技术创制广亲和水稻温敏雄性不育系[J]. 中国水稻科学, 2023, 37(3): 233-243.
[9]	程玲, 黄福钢, 邱一埔, 王心怡, 舒宛, 邱永福, 李发活. 籼稻材料570011抗褐飞虱基因的遗传分析及鉴定[J]. 中国水稻科学, 2023, 37(3): 244-252.
[10]	王文婷, 马佳颖, 李光彦, 符卫蒙, 李沪波, 林洁, 陈婷婷, 奉保华, 陶龙兴, 符冠富, 秦叶波. 高温下不同施肥量对水稻产量品质形成的影响及其与能量代谢的关系分析[J]. 中国水稻科学, 2023, 37(3): 253-264.
[11]	刘嫒桦, 李小坤. 不同肥料施用与稻米品质关系的整合分析[J]. 中国水稻科学, 2023, 37(3): 276-284.
[12]	杨晓龙, 王彪, 汪本福, 张枝盛, 张作林, 杨蓝天, 程建平, 李阳. 不同水分管理方式对旱直播水稻产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 285-294.
[13]	魏晓东, 宋雪梅, 赵凌, 赵庆勇, 陈涛, 路凯, 朱镇, 黄胜东, 王才林, 张亚东. 硅锌肥及其施用方式对南粳46产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 295-306.
[14]	林聃, 江敏, 苗波, 郭萌, 石春林. 水稻高温热害模型研究及其在福建省的应用[J]. 中国水稻科学, 2023, 37(3): 307-320.
[15]	郑承梅, 孙金秋, 刘梦杰, 杨永杰, 陆永良, 郭怡卿, 唐伟. 水稻田糠稷种子萌发和出苗特性及化学防除药剂筛选[J]. 中国水稻科学, 2023, 37(3): 321-328.

水稻单片段代换系对两种不育细胞质恢复性的遗传分析

Genetic Analysis of Restoration of Chromosome Single Segment Substitution Line to two Different CMS Lines in Rice

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics