关键词: 水稻, 无机焦磷酸化酶, 遗传转化, 源限制型品种, 库限制型品种

Abstract: OsIP1, encoding soluble inorganic pyrophosphatase was cloned and inserted between the rice mesophyllspecific promoter “cyFBPase” and the poly(A) site of the octopine synthase gene, and transferred into different sourcesink type rice cultivars, including sourcelimiting type Zhongchao 123 and sinklimiting type Nongken57 by Agrobacteriummediated gene transfer. Some T0 transgenic plants with high expression level were selected by quantitative realtime PCR and their T2 homozygous lines with the same morphological and agronomic traits as their  wildtype cultivar were generated. The effects of overexpressed OsIP1 specifically in the mesophyll cell were estimated by investigating tiller dynamics, soluble and stored carbohydrate contents in leaf blade and leaf sheath, and dry weight and yield and so on. The preliminary results showed that during the vegetative stage, the tilling capability and panicles per plant of transgenic lines increased to a certain extent, especially in the high tillering capability variety, Nongken 57. The results also showed that during the grainfilling stage, the changing trends of the soluble sugar, sucrose and starch contents in leaf blade and leaf sheath of transgenic lines were consistent with those of the wild types. Except leaf sheath at the peak grainfilling stage, the sucrose content of transgenic lines were increased significantly at each time point. Compared with the wild types, the dry weights and grain yields of transgenic lines increased to a varying degree. The grain yield of the transgenic lines of Zhongchao 123 were significantly higher than that of the wild type. 

Key words: rice, inorganic pyrophosphatase, genetic transformation, sourcelimiting type variety, sinklimiting type variety