利用重测序的水稻染色体片段代换系定位控制稻米淀粉黏滞性谱QTL

doi:10.3969/j.issn.10017216.2013.01.008

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (1): 56-64.DOI: 10.3969/j.issn.10017216.2013.01.008

利用重测序的水稻染色体片段代换系定位控制稻米淀粉黏滞性谱QTL

张昌泉,胡冰,朱孔志,张华,冷亚麟,汤述翥,顾铭洪,刘巧泉^*

扬州大学农学院江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室, 江苏扬州 225009;

收稿日期:2012-05-02 修回日期:2012-06-04 出版日期:2013-01-10 发布日期:2013-01-10
通讯作者: 刘巧泉*
基金资助:
国家973计划资助项目（2012CB944803）; 国家自然科学基金资助项目（31071383, 30971754）。

Mapping of QTLs for Rice RVA Properties Using Highthroughput Resequenced Chromosome Segment Substitution Lines

ZHANG Changquan, HU Bing, ZHU Kongzhi, ZHANG Hua, LENG Yalin, TANG Shuzhu, GU Minghong, LIU Qiaoquan^*

Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou University, Yangzhou 225009, China;

Received:2012-05-02 Revised:2012-06-04 Online:2013-01-10 Published:2013-01-10
Contact: LIU Qiaoquan*

摘要/Abstract

摘要： 稻米RVA谱是评价稻米蒸煮与食用品质的重要指标之一，发掘新的控制稻米RVA谱QTL对稻米品质改良具有重要意义。利用以粳稻品种日本晴为受体、籼稻品种9311为供体并经高通量重测序的染色体片段代换系群体为材料，在两年两点的环境下对该群体中控制RVA谱特征值的QTL进行了定位分析。通过单因素方差分析和Dunnett多重比较, 分析染色体片段代换系与受体亲本之间相关RVA谱特征值的差异，以两年两点都能检测到的显著差异位点作为稳定表达的QTL。共检测到10个稳定表达的QTL，包括控制峰值黏度的4个QTL qPKV2.1、qPKV5.1、qPKV7.1和qPKV8.1，控制热浆黏度的2个QTL qHPV5.1和qHPV7.1，控制冷胶黏度的2个QTL qCPV5.1和qCPV7.1及控制消减值的2个QTL qSBV2.1和qSBV7.1。在两年两点的检测中10个稳定表达QTL的贡献率介于-31.8%～53.2%，这10个QTL分布在第2、5、7和8染色体上，其中位于第2、5和7染色体上的位点存在一因多效性。

关键词: 水稻, 染色体片段代换系； RVA谱, QTL；代换作图

Abstract: The RVA profile is one of the important factors for rice cooking and eating quality evaluation, and it would be of great significance to identify new quantitative trail loci (QTLs) for RVA profile for improvement of rice quality. With Nipponbare as recipient and 9311 as donor, a population with 38 chromosome segment substitution lines (CSSLs) had been developed and subsequently genotyped by highthroughput resequencing strategy. The population with their parents similar in apparent amylose content was used for mapping QTLs of RVA properties including peak paste viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BDV), setback viscosity (SBV), consistence viscosity (CSV), peak time (PeT) and pasting temperature (PaT) in two years and two environments. The QTL analysis was carried out by using oneway ANOVA and Dunnett’s test, and stable QTLs were identified in both two years and two environments. 10 stable QTLs, qPKV2.1, qPKV5.1, qPKV7.1, qPKV8.1, qHPV5.1, qHPV7.1, qCPV5.1, qCPV7.1, qSBV2.1 and qSBV7.1 were identified in the chromosome 2, 5, 7 and 8, respectively, with contributions ranging from -31.8% to 53.2%. The QTLs in chromosome 2, 5 and 7 were found to have pleiotropy.

Key words: rice, chromosome segment substitution line, RVA profile, quantitative trait locus, substitution mapping

中图分类号:

张昌泉,胡冰,朱孔志,张华,冷亚麟,汤述翥,顾铭洪,刘巧泉*. 利用重测序的水稻染色体片段代换系定位控制稻米淀粉黏滞性谱QTL[J]. 中国水稻科学, 2013, 27(1): 56-64.

ZHANG Changquan, HU Bing, ZHU Kongzhi, ZHANG Hua, LENG Yalin, TANG Shuzhu, GU Minghong, LIU Qiaoquan*. Mapping of QTLs for Rice RVA Properties Using Highthroughput Resequenced Chromosome Segment Substitution Lines[J]. Chinese Journal of Rice Science, 2013, 27(1): 56-64.

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利用重测序的水稻染色体片段代换系定位控制稻米淀粉黏滞性谱QTL

Mapping of QTLs for Rice RVA Properties Using Highthroughput Resequenced Chromosome Segment Substitution Lines

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