水稻ABCB转运蛋白基因的分子进化和表达分析

doi:10.3969/j.issn.10017216.2012.02.001

摘要/Abstract

摘要： 系统鉴定了水稻和拟南芥中27个和29个ABC家族B亚族（ABCB）基因，发现其外显子数目、编码蛋白质的长度和分子量在两种植物中均存在较大变异，而等电点分布的变化较小。系统发育分析把该亚族蛋白分为4个亚组，说明其功能可能已经发生了分化；在水稻和拟南芥中各鉴定了6对和9对旁系同源基因，说明单、双子叶植物分离之后，该亚族在水稻和拟南芥中均以物种特异的方式进行了扩张。片段复制和串联重复是水稻ABC家族扩张的主要机制。多序列比对显示，核苷酸结合域（NBD）的Walker A、Walker B和ABC域基序均高度保守，而Q环和H环的保守性略差；跨膜结构域（TMD）在不同蛋白之间和同一蛋白内没有明显的保守性。表达模式分析表明，水稻ABCB基因的表达具有明显的组织特异性，不同基因的表达特征已经发生分化。非生物胁迫下的表达分析显示多数水稻ABCB基因受到至少一种非生物胁迫因素的调控。旁系同源基因Ka/Ks分析表明，净化选择在水稻ABCB基因复制后功能的保留中发挥了重要作用。

关键词: 水稻, ABC转运蛋白, 进化, 表达分析

Abstract: With the accomplishment of genome sequencing projects, over 130 ABCs were identified separately from the model plants of monocots and dicots, rice and Arabidopsis, but the functions of most of the members remain elusive. Therefore, 27 and 29 subfamily B of ABC genes (ABCB) in rice and Arabidopsis were systematically characterized, respectively. The exon number, length and molecular weight of proteins encoded by these genes varied greatly between the two plants, albeit the isoelectric point was less diverse. Proteins of this subfamily were divided into 4 subgroups accordingpar to phylogenetic analysis, suggesting that divergence probably occurred among them; 6 and 9 pairs of paralogous genes were identified from rice and Arabidopsis, respectively, indicating that speciesspecific expansion contributed to the evolution of this subfamily in rice and Arabidopsis after the split of monocots and dicots. Segmental and tandem duplication contributed to the amplification of ABC family in rice. Multiple sequence alignment revealed that the Walker A, Walker B, and ABC signature motifs of nucleotidebinding domain (NBD) are highly conserved, but less the Q and H loops; no significant conservatism could be pointed out as to TMD domain within a single ABC protein and among different proteins. Expression of rice ABCB genes is highly tissuespecific, and divergence has occurred among the expression profiles of different genes. Expression analysis of rice ABCB genes under abiotic stress suggested that most of the genes are responsive to at least 1 type of stress factors.Ka/Ks calculation indicated that purifying selection is essential to the functional maintenance of genes after duplication.

Key words: rice, ABC transporter, evolution, expression analysis

中图分类号:

Q3431+5

徐杏,邱杰,徐扬,徐辰武*. 水稻ABCB转运蛋白基因的分子进化和表达分析[J]. 中国水稻科学, 2012, 26(2): 127-136.

XU Xing, QIU Jie, XU Yang, XU Chenwu*. Molecular Evolution and Expression Analysis of Subfamily ABCB Transporter Genes in Rice[J]. Chinese Journal of Rice Science, 2012, 26(2): 127-136.

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