Genetic Analysis of Heading Date of Late Season Japonica Rice from Taihu Lake Region
ZHOU Zhen-Ling1, 2 , JIANG Ling1,Xu Da-Yong1, 2 , WEI Xiang-Jin1, LIU Xi1, LU Bai-Guan2, WAN Jian-Min1, 3 , *
1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2Lianyungang Academy of Agricultural Sciences, Lianyungang 222006, China; 3 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Heading dates of 20 late season japonica rice cultivars native to the Taihu Lake region of China were investigated, and their basic vegetative growth (BVG), photoperiodsensitivity (PS) and temperaturesensitivity (TS) were analyzed under artificial shortday and natural longday conditions in Nanjing, as well as artificial high temperature and natural low temperature conditions in winter in Hainan. The results showed that all the cultivars had strong PS, and most of the cultivars had strong TS and short BVG. Regression analysis showed a significant correlation between heading date and PS, indicating PS was the main factor affecting heading date of late season japonica rice cultivars from the Taihu Lake region. Genetic analyses were conducted by using a set of heading date near isogenic lines as tester lines. All these japonica cultivars carried the dominant photoperiod sensitivity allele E1 and the dominant earlyheading gene Ef1. At the E2 and E3 loci, most of the late season japonica cultivars carried the photoperiod insensitivity alleles e2 and e3, but some carried the photoperiod sensitivity alleles E2 and E3 or E3t. At the Se1 locus, these late season japonica cultivars mainly carried recessive photoperiod insensitivity gene Se1e, others carried Se1n. In addition, some of the late season japonica cultivars carried the recessive allele hd2, inhibiting the expressions of E1 and Se-1.
ZHOU Zhen-Ling-1,2 ,JIANG Ling-1Xu-Da-Yong-1 et al. Genetic Analysis of Heading Date of Late Season Japonica Rice from Taihu Lake Region[J]. , 2011, 25(4): 357-364.
Kinoshita T. Report of committee on gene symbolization, nomenclature and linkage groups. Rice Genet Newsl, 1995, 12: 25-125.
Maheswaran M, Mackill D J, Huang N, et al.Identification of RAPD markers linked to Se-3(t), a gene enhancing the level of photoperiod sensitivity in rice. Rice Genet Newsl, 1995, 12: 219-221.
Ohshima I, Watanabe Y, Asahic C. Genetic analysis of heading time in cross between twoindica varieties with two inhibitor genes for photoperiod sensitivity. Jpn J Breed, 1993, 43: 101-106.
Okumoto Y, Tanisaka T, Yamagata H. Heading-time genes of the rice varieties grown in the Tohoku-Hokuriku region in Japan. Jpn J Breed, 1992, 42: 121-135.
Okumoto Y, Tanisaka T. Trisomic analysis of a strong photoperiod-sensitivity gene E1 in rice (Oryza sativa L.).Euphytica, 1997, 95: 301-307.
Yokoo M, Okuno K. Genetic analysis of earliness mutations induced in the rice cultivar Norin 8. Jpn J Breed, 1993, 43: 1-11.
Shen B, Qian H R, Wang J L. Tagging genes for heading date in rice via linkage to RFLP markers. Acta Agron Sin,1994,20(1): 1-7.
Ichitani K, Okumoto Y, Tanisaka T. Photoperiod sensitivity gene of Se-1locus found in photoperiod insensitive rice cultivars of the northern limit region of rice cultivation. Breed Sci,1997, 47: 145-152.
Okumoto Y, Ichitani K, Inoue H. Photoperiod insensitivity gene essential to the varieties grown in the northern limit region of paddy rice (Oryza sativaL.) cultivation. Euphytica, 1996, 92: 63-66.
Yamagata H, Okumoto Y, Tanisaka T. Analysis of genes controlling heading time in Japanese rice//Rice Genetics. Manila, Philippines: IRRI, 1986: 351-359.
Ohshima I, Kikuchi F. Identification of a recessive inhibitor for photoperiod-sensitive gene, Se-1, in photoperiod-insensitive varieties of indica type rice. Proceeding of 7th International CongressSAERAO, 1994: 93-100.
Tsai K H. Gene loci and alleles controlling the duration of basic vegetative growth of rice//Rice Genetics.Manila, Philippines: IRRI, 1986: 339-349.
Inoue I, Nishida H, Okumoto Y, et al.Identification of an early heading time gene found in the Taiwanese rice cultivar Taichung 65. Breed Sci,1998, 48: 103-108.
Ichitani K, Okumoto Y, Tanisaka T. Genetic analyses of low photoperiod sensitivity of rice cultivars from the northern most regions of Japan. Plant Breed, 1998, 117: 543-547.
Wei X J, Jiang L, Xu J F, et al. Genetic analyses of heading date ofjaponica rice cultivars from Northeast China. Field Crops Res,2008, 107: 147-154.
Ichitani K, Okumoto Y, Tanisaka T. Genetic analysis of the rice cultivar Kasalath with special reference to two photoperiod sensitivity loci E1and Se-1. Breeding Sci， 1998,48: 51-57.
Lin H X, Yamamoto T, Sasaki T, et al. Characterization and detection of epistatic interaction three QTLs, Hd-1， Hd-2andHd-3, controlling heading date of rice using nearly isogenic lines. Theor Appl Genet,2000, 101: 1021-1028.
Yamamoto T, Kuboki Y, Lin S Y. Fine mapping of quantitative trait loci Hd-1，Hd-2andHd-3， controlling heading date of rice, as single Mendellian factors. Theor Appl Genet， 1998, 97: 37-44.
Nishida H, Okumoto Y, Nakagawa H, et al. Analysis of tester lines for rice (Oryza sativaL.) heading-time genes using reciprocal photoperiodic transfer treatments. Ann Bot, 2001, 88: 527-536.
Okumoto Y, Yoshimura A, Tanisaka T, et al. Analysis of a rice variety Taichung 65 and its isogenic early-heading lines for late-heading genes E1， E2 and E3.Jpn J Breed， 1992, 42: 415-429.
Yokoo M, Kikuchi F. Multiple allelism of the locus controlling heading time of rice, detected using the close linkage with the blast-resistance. Jpn J Breed， 1977, 27: 123-130.
Yano M, Katayose Y, Ashikari M, et al. Hd1， a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering line gene CONSTANS. Plant Cell，2000, 12: 2473-2483.
Yano M, Harushima Y, Nagamura Y, et al. Identification of quantitative trait loci controlling heading date in rice using a high-density linkage map. Theor Appl Genet， 1997, 95: 1025-1032.