粳稻发芽期耐碱性的QTL检测

doi:10.3969/j.issn.1001-7216.2009.06.05

中国水稻科学

粳稻发芽期耐碱性的QTL检测

祁栋灵^1,2,李丁鲁³,杨春刚⁴,李明哲³,曹桂兰¹,张俊国⁴,周庆阳⁵,徐锡哲^3,张三元^4，*.韩龙植^1，*

¹中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室，北京 100081； ²中国热带农业科学院橡胶研究所/国家重要热带作物工程技术研究中心/省部共建国家重点实验室培育基地海南省热带作物栽培生理学重点实验室，海南儋州571737； ³韩国农村振兴厅国立农业科学院，韩国水原 441-707； ⁴吉林省农业科学院水稻研究所，吉林公主岭 136100； ⁵四川农业大学园艺系，四川雅安625014； *通讯联系人， E-mail: lzhan58@yahoo.com.cn; jlgzszsy12@126.com

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-11-10 发布日期:2009-11-10

Detection of QTL for Alkali Tolerance at the Germination Stage in japonica Rice

QI Dong-ling^{1, 2}, LEE Jung-ro³, YANG Chun-gang⁴, LEE Myung-chul³, CAO Gui-lan¹, ZHANG Jun-guo⁴, ZHOU Qing-yang⁵, SUH Seok-cheol³, ZHANG San-yuan^4，*, HAN Long-zhi^1，*

¹Institute of Crop Science, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI/Key Laboratory of Crop Germplasm Resources and Biotechnology, Ministry of Agriculture, Beijing 100081, China； ²Institute of Rubber Research, Chinese Academy of Tropical Agricultural Sciences/State Engineering and Technology Research Center for Key Tropical Crops/State Key Laboratory Breeding Base of Cultivation & Physiology for Tropical Crops,Hainan Provincial Key Laboratory for Tropical Crops Physiology, Danzhou 571737, China; ³National Academy of Agricultural Sciences, RDA, Suwon 441707, Korea； ⁴Institute of Rice Research, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China； ⁵Horticultural Department, Sichuan Agricultural University, Ya′an 625014, China; *Corresponding authors, E-mail: lzhan58@yahoo.com.cn, jlgzszsy12@126.com

Received:1900-01-01 Revised:1900-01-01 Online:2009-11-10 Published:2009-11-10

摘要/Abstract

摘要： 以粳粳交高产106/长白9号的200个F2:3株系为作图群体，在0.15% Na2CO3溶液碱胁迫下，进行了水稻发芽率及其相对碱害率的鉴定评价，并以SSR标记构建的分子连锁图谱为基础，对水稻发芽率及其相对碱害率进行了数量性状基因座(QTL)检测。结果表明，在F3株系群中水稻发芽率及其相对碱害率均呈单峰接近正态的连续分布。共检测到碱胁迫下与水稻发芽率相关的QTL 7个，对表型变异的贡献率范围为4.05%～12.61%，其中位于第6染色体RM225-RM204区间的qGC6和位于第9染色体RM219-RM3700区间的qGC9对表型变异的贡献率分别为12.61%和10.85%。共检测到与水稻发芽率相对碱害率相关的QTL 6个，对表型变异的贡献率为4.82%～28.07%，其中位于第2染色体RM29-RM221区间的qRGC2、位于第6染色体RM225-RM204区间的qRGC61、位于第9染色体RM219-RM3700区间的qRGC9和位于第12染色体RM260-RM3226区间的qRGC12对表型变异的贡献率较大，分别为28.07％、15.35％、15.61％和18.91％，为主效QTL，但其相应的区间距离均较远，需要进一步深入研究。所检测的QTL增效等位基因主要表现为部分显性和超显性。

关键词: 水稻, 耐碱性, 发芽率, 相对碱害率, 微卫星标记, 数量性状基因座

Abstract: The quantitative trait loci (QTLs) for germination capacity and relative alkali damage rate for germination capacity in rice under alkali stress (0.15% Na2CO3 solution) were identified by using an F2:3 population, which included 200 individuals and lines derived from a cross between two japonica cultivars Gaochan 106 and Changbai 9 with microsatellite markers. The germination capacity and its relative alkali damage rate showed a continuous and near normal distribution in F3 lines. Seven QTLs associated with the germination capacity under alkali stress were detected, which explained 4.05% to 12.61% of the observed phenotypic variances. qGC6 and qGC9 were located in RM225-RM204 and RM219-RM3700, respectively, which accounted for 12.61% and 10.85% of the observed phenotypic variation, respectively. Six QTLs correlated with relative alkali damage rate for germination capacity under alkali stress were detected. qRGC2, qRGC61, qRGC9, and qRGC12 were major QTLs located in RM29-RM221, RM225-RM204, RM219-RM3700, and RM260-RM3226, respectively, which explained 28.07%, 15.35%, 15.61% and 18.91% of the observed phenotypic variation, respectively. Given the long distances between the markers further research should be conducted. Most of the alleles were partially dominant or overdominant.

Key words: rice, alkali tolerance, germination capacity, relative alkali damage rate, microsatellite marker, quantitative trait locus

祁栋灵李丁鲁,杨春刚,李明哲,曹桂兰,张俊国,周庆阳,徐锡哲,张三元,.韩龙植,. 粳稻发芽期耐碱性的QTL检测[J]. 中国水稻科学 2009,23(6): 589-594 . , DOI: 10.3969/j.issn.1001-7216.2009.06.05 .

QI Dong-ling,LEE Jung-ro,YANG Chun-gang,LEE Myung-chul,CAO Gui-lan,ZHANG Jun-guo,ZHOU Qing-yang,SUH Seok-cheol,ZHANG San-yuan,HAN Long-zhi. Detection of QTL for Alkali Tolerance at the Germination Stage in japonica Rice[J]. Chinese Journal of Rice Science 2009,23(6): 589-594 ., DOI: 10.3969/j.issn.1001-7216.2009.06.05 .

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粳稻发芽期耐碱性的QTL检测

Detection of QTL for Alkali Tolerance at the Germination Stage in japonica Rice

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