水稻第6染色体短臂上株高QTL qPH6-1的精细定位

doi:10.3969/j.issn.10017216.2009.05.04

中国水稻科学

水稻第6染色体短臂上株高QTL qPH6-1的精细定位

鲍钱江^{1, 2},樊叶杨¹,於卫东²,陈忱¹,范方军¹,杜景红¹,庄杰云^{1, *}

¹中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室，浙江杭州 310006； ²杭州师范大学生命与环境科学学院, 浙江杭州 310036； *通讯联系人， E-mail: jz1803@hzcnc.com

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-09-10 发布日期:2009-09-10

Fine Mapping of QTL qPH6-1 for Plant Height on the Short Arm of Rice Chromosome 6

BAO Qian-jiang^{1, 2}, FAN Ye-yang¹, YU Wei-dong², CHEN Chen¹, FAN Fang-jun¹, DU Jing-hong¹, ZHUANG Jie-yun^{1, *}

¹Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; ²College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; * Corresponding author, E-mail: jz1803@hzcnc.com

Received:1900-01-01 Revised:1900-01-01 Online:2009-09-10 Published:2009-09-10

摘要/Abstract

摘要： 应用衍生于水稻剩余杂合体的分离群体，开展株高QTL的检测和精细定位。应用1个在水稻第6染色体短臂约7.3 Mb区间分离、背景基本纯合的F2:3群体，种植于海南、浙江两地，检测到2个控制株高的QTL；然后，针对两地间作用稳定的qPH61，筛选出3个杂合区间缩小且呈阶梯状排列的单株，衍生F2群体，进一步验证了qPH61的作用，并将其界定于距离为96.4 kb的SSR标记RM3414和RM19417之间；最后，应用分离区间进一步缩小且呈阶梯状排列的3个F2群体，将qPH61定位于距离为51.7 kb的STS标记Si2925和SSR标记RM19417之间。基因组位置比较结果显示，该基因与所有已定位或克隆的水稻矮秆、半矮秆基因均非等位。

关键词: 株高, 数量性状基因座, 精细定位, 剩余杂合体, 水稻

Abstract: Populations derived from residual heterozygous lines (RHLs) were used for the detection and fine mapping of quantitative trait loci (QTLs) for plant height in rice. An F2:3 population which segregates in a 7.3Mb region on the short arm of rice chromosome 6 in an isogenic background were transplanted in two trial sites in Hainan and Zhejiang Provinces, respectively. Two QTLs for plant height were detected, of which qPH61 exhibited a consistent effect across the two sites. Three plants having smaller heterozygous segments which overlap the qPH61 region were selected. Using the three F2 populations produced, qPH61 was validated and delimitated to a 96.4kb region flanked by SSR markers RM3414 and RM19417. Three more F2 populations which segregate in smaller regions covering qPH61 were developed. The qPH61 locus was delimited to a 51.7kb region flanked by STS marker Si2925 and SSR marker RM19417. It was also shown that this gene is nonallelic to all the major genes for dwarfism mapped or cloned in rice.

Key words: plant height, quantitative trait locus, fine mapping, residual heterozygous line, rice

鲍钱江樊叶杨,於卫东,陈忱,范方军,杜景红,庄杰云, . 水稻第6染色体短臂上株高QTL qPH6-1的精细定位
[J]. 中国水稻科学 2009,23(5): 470-474 . , DOI: 10.3969/j.issn.10017216.2009.05.04 .

BAO Qian-jiang,FAN Ye-yang,YU Wei-dong,CHEN Chen,FAN Fang-jun,DU Jing-hong,ZHUANG Jie-yun. Fine Mapping of QTL qPH6-1 for Plant Height on the Short Arm of Rice Chromosome 6[J]. Chinese Journal of Rice Science 2009,23(5): 470-474 ., DOI: 10.3969/j.issn.10017216.2009.05.04 .

参考文献

[1]Khush G S. Green revolution: Preparing for the 21st century. Genome, 1999, 42: 646-655.

[2]Hedden P. The genes of the green revolution. Trends Genet, 2003, 19: 5-9.

[3]马良勇, 包劲松, 李西明, 等. 水稻矮生基因的克隆和功能研究进展. 中国水稻科学, 2009, 23(1): 1-11.

[4]Huang N, Courtois B, Khush G S, et al. Association of quantitative trait loci for plant height with major dwarfing genes in rice. Heredity, 1996, 77: 130-137.

[5]袁隆平. 选育水稻亚种间杂交组合的策略. 杂交水稻, 1996, 16(2): 1-3.

[6]杜景红, 樊叶杨, 吴季荣, 等. 水稻第6染色体短臂产量性状QTL簇的分解. 中国农业科学, 2008, 41(4): 939-945.

[7]Zheng K L, Huang N, Bennett J, et al. PCRbased markerassisted selection in rice breeding. IRRI Discussion Paper Series No.12. Manila: International Rice Research Institute, 1995.

[8]施勇烽, 应杰政, 王磊, 等. 鉴定水稻品种的微卫星标记筛选. 中国水稻科学, 2005, 19(3): 195-201.

[10]Lander E, Green P, Abrahamson J, et al. MAPMAKER: An interactive computer package for constructing primary genetic maps of experimental and natural populations. Genomics, 1987, 1: 174-181.

[11]Wang S, Basten C J, Zeng Z B. Windows QTL Cartographer 2.5. Raleigh, USA： Department of Statistics, North Carolina State University, 2007.

[12]Li Z K, Pinson S R M, Stansel J W, et al. Identification of quantitative trait loci (QTLs) for heading date and plant height in cultivated rice (Oryza sativa L.). Theor Appl Genet, 1995, 91: 374381.

[13]Yan J Q, Zhu J, He C X, et al. Molecular dissection of developmental behavior of plant height in rice (Oryza sativa L.). Genetics, 1998, 150: 1257-1265.

[14]邢永忠, 徐才国, 华金平, 等. 水稻株高和抽穗期基因的定位和分离. 植物学报, 2001, 43(7): 721-726.

[15]Cao G Q, Zhu J, He C X, et al. Impact of epistasis and QTL×environment interaction on the developmental behavior of plant height in rice (Oryza sativa L.). Theor Appl Genet, 2001, 103: 153-160.

[16]Thomson M J, Tai T H, McClung A M, et al. Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet, 2003, 107: 479-493.

[17]Septiningsih E M, Prasetiyono J, Lubis E, et al. Identification of quantitative trait loci for yield and yield components in an advanced backcross population derived from the Oryza sativa variety IR64 and the wild relative O. rufipogon. Theor Appl Genet, 2003, 107: 1419-1432.

[18]Mei H W, Li Z K, Shu Q Y, et al. Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two backcross populations. Theor Appl Genet, 2005, 110: 649-659.

[19]赵芳明, 张桂权, 曾瑞珍, 等. 用单片段代换系(SSSLs)研究水稻株高及其构成因素QTL加性及上位性效应. 作物学报, 2009, 35(1): 48-56.

[20]Yano M, Katayose Y, Ashikari M, et al. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell, 2000， 12: 2473-2483.

[1]	任志奇, 薛可欣, 董铮, 李小湘, 黎用朝, 郭玉静, 刘文强, 郭梁, 盛新年, 刘之熙, 潘孝武. 水稻外卷叶突变体ocl1的鉴定及基因定位[J]. 中国水稻科学, 2023, 37(4): 337-346.
[2]	肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A/bar*基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358.
[3]	李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367.
[4]	汪胜勇, 陈宇航, 陈会丽, 黄钰杰, 张啸天, 丁双成, 王宏伟. 水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响[J]. 中国水稻科学, 2023, 37(4): 368-378.
[5]	董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404.
[6]	韩聪, 何禹畅, 吴丽娟, 郏丽丽, 王磊, 鄂志国. 水稻碱性亮氨酸拉链(bZIP)蛋白家族功能研究进展[J]. 中国水稻科学, 2023, 37(4): 436-448.
[7]	沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232.
[8]	段敏, 谢留杰, 高秀莹, 唐海娟, 黄善军, 潘晓飚. 利用CRISPR/Cas9技术创制广亲和水稻温敏雄性不育系[J]. 中国水稻科学, 2023, 37(3): 233-243.
[9]	程玲, 黄福钢, 邱一埔, 王心怡, 舒宛, 邱永福, 李发活. 籼稻材料570011抗褐飞虱基因的遗传分析及鉴定[J]. 中国水稻科学, 2023, 37(3): 244-252.
[10]	王文婷, 马佳颖, 李光彦, 符卫蒙, 李沪波, 林洁, 陈婷婷, 奉保华, 陶龙兴, 符冠富, 秦叶波. 高温下不同施肥量对水稻产量品质形成的影响及其与能量代谢的关系分析[J]. 中国水稻科学, 2023, 37(3): 253-264.
[11]	刘嫒桦, 李小坤. 不同肥料施用与稻米品质关系的整合分析[J]. 中国水稻科学, 2023, 37(3): 276-284.
[12]	杨晓龙, 王彪, 汪本福, 张枝盛, 张作林, 杨蓝天, 程建平, 李阳. 不同水分管理方式对旱直播水稻产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 285-294.
[13]	魏晓东, 宋雪梅, 赵凌, 赵庆勇, 陈涛, 路凯, 朱镇, 黄胜东, 王才林, 张亚东. 硅锌肥及其施用方式对南粳46产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 295-306.
[14]	林聃, 江敏, 苗波, 郭萌, 石春林. 水稻高温热害模型研究及其在福建省的应用[J]. 中国水稻科学, 2023, 37(3): 307-320.
[15]	郑承梅, 孙金秋, 刘梦杰, 杨永杰, 陆永良, 郭怡卿, 唐伟. 水稻田糠稷种子萌发和出苗特性及化学防除药剂筛选[J]. 中国水稻科学, 2023, 37(3): 321-328.

水稻第6染色体短臂上株高QTL qPH6-1的精细定位

Fine Mapping of QTL qPH6-1 for Plant Height on the Short Arm of Rice Chromosome 6

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics