中国水稻科学

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水稻第6染色体短臂谷壳硅含量QTL的分解

吴季荣,范方军,杜景红,樊叶杨,庄杰云*   

  1. 中国水稻研究所 国家水稻改良中心/水稻生物学国家重点实验室, 浙江 杭州 310006;*通讯联系人, E-mail: jz1803@hzcnc.com
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-10 发布日期:2010-01-10

Dissection of QTLs for Hull Silicon Content on the Short Arm of Rice Chromosome 6

WU Ji-rong, FAN Fang-jun, DU Jing-hong, FAN Ye-yang, ZHUANG Jie-yun*   

  1. Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; * Corresponding author, E-mail: jz1803@hzcnc.com
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-10 Published:2010-01-10

摘要: 报道了水稻谷壳硅含量QTL qHUS6的分解研究。针对前期定位的qHUS6区域,应用在第6染色体短臂RM587-RM19784区间分离、背景基本纯合的F2:3群体,将qHUS6分解为2个QTL;其中,qHUS61位于目标区间的上部,qHUS62位于下部。同时,筛选出在目标区间内携带更小杂合片段的3个单株。其中,2株覆盖qHUS61区间,各自交产生了1个F2:3群体,应用这2个群体将qHUS61定位于RM510和RM19417之间约147.0 kb的区间内;另1株覆盖qHUS62区间,自交产生了1个F2:3群体,从中挑选出在qHUS62区间具有不同基因型组成的5套F3株系,将qHUS62分解为qHUS62a和qHUS62b,分别位于RM19706-RM19795和RM314-RM19665区间。
关键词:

关键词: 水稻, 数量性状基因座, 剩余杂合体, 谷壳硅含量

Abstract:
The dissection of the QTL qHUS6 for hull silicon content in rice which was previously located on the short arm of rice chromosome 6 is reported. Using an F2:3 population segregated in the RM587-RM19784 region harboring qHUS6 in an isogenic background, two QTLs for hull silicon content were detected, of which qHUS61 was located in the upper region and qHUS62 in the lower region. Three plants carrying smaller heterozygous segments in the target region were selected, of which two covered the qHUS61 region and the other covered the qHUS62 region. Three F2:3 populations were derived from the selfed seeds of the three plants, respectively. QTL mapping was performed using the two populations segregated in the qHUS61 region, and qHUS61 was delimited to a 147.0 kb region flanked by RM510 and RM19417. Five groups of F3 lines with different genotypic composition in the qHUS62 region were selected from the other F2:3 population. Two QTLs were separated with twoway ANOVA, of which qHUS62a was located in the interval RM19706-RM19795 and qHUS62b in the interval RM314-RM19665.

Key words: rice (Oryza sativa), quantitative trait locus, residual heterozygous line, hull silicon content, gene mapping