中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

太湖流域粳稻地方品种的微卫星分析

于 萍1;李 丽2; 吕建珍1;袁筱萍1;徐 群1;王一平1;余汉勇1;魏兴华1,*   

  1. 1中国水稻研究所 水稻生物学国家重点实验室， 浙江 杭州310006； 2山西农业大学， 山西 太谷030801； *通讯联系人， E-mail: xwei@mail.hz.zj.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-10 发布日期:2009-03-10

SSR Analysis on japonica Rice Landraces from the Taihu Lake Region, China

YU Ping1, LI Li2, LU Jianzhen1, YUAN Xiaoping1, XU Qun1, WANG Yiping1, YU Hanyong1, WEI Xinghua1,*   

  1. 1 State Key Laboratory of Rice Biology， China National Rice Research Institute， Hangzhou 310006， China； 2 Shanxi Agricultural University, Taigu 030801, China； * Corresponding author， E-mail: xwei@mail.hz.zj.cn
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-10 Published:2009-03-10

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摘要： 采用45对SSR引物对224份太湖流域粳稻地方品种进行遗传多样性分析，共检测到162个等位变异，每个位点等位基因数的变幅为2～7，平均为3.6。各位点Nei基因多样性指数变异较大，为0.009（RM169）～0.663（RM444），平均为0.197。青稻、黄稻、红稻和白稻各传统生态型均具较低的遗传多样性。分子方差分析表明，SSR遗传变异绝大部分存在于传统生态型内。青稻与红稻间、青稻与白稻、红稻与白稻、红稻与黄稻间遗传分化显著。太湖流域粳稻地方品种SSR多样性较低，稀有类型等位基因较多。这对科学制定太湖粳稻地方资源的保护和利用策略具有指导意义。

关键词: 太湖地区, 粳稻, 地方品种, 微卫星标记, 遗传多样性, 分子方差分析

Abstract: Fortyfive SSR markers were used to study the genetic diversity in 224 japonica rice landraces from the Taihu Lake Region, China. There were 162 alleles at the 45 loci investigated. The number of alleles per locus (Na) ranged from 2 to 7, with a mean value of 3.6. The Nei′s genetic diversity index (He) varied widely from 0.009 (RM169) to 0.663 (RM444) with an average value of 0.197. The genetic diversity of all the traditional ecotypes (green rice, yellow rice, red rice and white rice) was low. Analysis of molecular variance (AMOVA) indicated that most of the genetic variation was from differences among traditional ecotypes. The pairwise comparison of Fstatistics(Fst) revealed a significant genetic differentiation in the four pairs (green rice vs red rice; green rice vs white rice; red rice vs white rice; red rice vs yellow rice), whereas no significant differentiation was found between other types. The results showed that more rare alleles and low genetic diversity were detected in japonica rice landraces from the Taihu Lake Region, China. This will be helpful to conserve and exploit Taihu japonica rice landraces.

Key words: Taihu Lake Region, japonica rice, landrace, simple sequence repeat, genetic diversity, analysis of molecular variance