1 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R & D Center, Nanjing 210014, China; 2 Agricultural College, Nanjing Agricultural University, Nanjing 210095, China；*Corresponding author, E-mail: firstname.lastname@example.org
Genetic diversity among 30 japonica rice was studied using the simple sequence repeat(SSR) markers, and relationship between genetic distance based on SSR markers and yield heterosis was analyzed using the materials consisted of 30 parents and 200 F1 hybrids with a 20×10 diallel crossing design. The results indicated that 64 pairs of SSR primers could produce 185 steady polymorphic bands among 30 parents averaging 2.9 bands for each pair of primers. The polymorphic index content (PIC) values varied from 0.064 to 0.844 with an average of 0.380. Cluster analysis by UPGMA classified 30 parents into seven groups based on genetic similarity, which was coincident with the heterotic groups determined by their pedigree analysis. The correlations between the genetic distance and yield heterosis and hybrid performance were significant except for the total number of spikelets per panicle, and the coefficients of correlations were ranged from -0.361 to 0.359. It is suggested that the molecular markers could be used as a useful tool for assigning heterotic group and measuring genetic diversity, but not for predicting heterosis because the coefficient of relationship between the genetic distance based on SSR markers and yield heterosis was too small.
ZHAO Qingyong ,ZHU Zhen ,ZHANG Yadong et al. Analysis on Correlation Between Heterosis and Genetic Distance Based on Simple Sequence Repeat Markers in japonica Rice[J]. , 2009, 23(2): 141-141～147 .
Lee M, Godshalk E B, Lamkey K R, et al. Association of restriction fragment length polymorphisms among maize in breeds with agronomic performance of their crosses. Crop Sci, 1989, 29: 1067-1071．
Smith O S, Smith J S C, Bowen S L, et al．Similarities among a group of elite maize in breeds as measured by pedigree, F1 grain yield, grain yield heterosis and RFLPs．Theor Appl Genet, 1990, 80: 833-840.
Dudley J W, Saghai Maroof M A, Rufener G K. Molecular marker and grouping of parents in maize breeding pograms. Crop Sci, 1991, 31: 718-723.
Godshalk E B, Lee M, Lamkey K R. Relationship of restriction fragment length polymorphisms to single cross hybrid performance of maize．Theor Appl Genet, 1990, 80: 273-280.
Melchinger A E, Lee M, Lamkey K R, et al．Genetic diversity for restriction fragment length polymorphisms and heterosis for two tiller sets of maize in breeds. Theor Appl Genet, 1990, 80: 488-496．
Boppenmaier J, Melchinger A E, BrunklausJune E, et al. Genetic diversity for RFLP in European maize in breeds: Ⅰ．Relation to performance of flint×dent crosses for forage trait．Crop Sci, 1992, 32: 895-902.
Zhang Q F, Zhou Z Q, Yang G P, et al．Molecular marker heterosis gosity and hybrid performance in indica and japonica rice．Theor Appl Genet, 1996, 93: 1218-1224．
Xiao J, Li J, Yuan L, et al．Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCRbased markers．Theor Appl Genet, 1996, 92: 637-643．
Dellaporta S L, Wood J, Hicks J B. A plant DNA minipreparation: Version Ⅱ. Plant Mol Biol Rep, 1983, 1(1): 19-21.
Nei M, Li W．Mathematical model for studying genetic variation in terms of restriction endonuclease. Proc Natl Acad Sci USA, 1979, 76: 5269-5273.
Rolf F J.NTSYSpc Numerical Taxonomy and Multivariate Analysis System. Version 1.80. New York: Exeter Publication, 1990.
Smith J S C, Chin E C L, Shu H, et al. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.) : Comparisons with data from RFLPs and pedigree. Theor Appl Genet, 1997, 95: 163-173.
Melchinger A E, Lee M, Lamkcy K R, et al．Genetic diversity for restriction fragment length polymorphisms: Relation to estimated genetic effects in maize inbreds．Crop Sci, 1990, 30: 1033-1040.