Breeding of Rice Restorer Lines Carrying Blast Resistance Gene Pi25 by MarkerAssisted Selection
ZHOU Haipeng1,#, ZHAN Xiaodeng1,#, CAI Rongyao2, CHENG Shihua1,*, CAO Liyong1,*
1Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China；2Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China； #These authors contributed equally to this paper; *Corresponding authors, E-mail: email@example.com；firstname.lastname@example.org
Three recombinant inbred lines (RILs) carrying rice blast resistance gene Pi25 from the combination Zhong 156/Gumei 2 were crossed with highyielding restorer lines, 9308 and 311. The seeds were collected and advanced by single seed descent, and six RIL populations at the F6 generation were obtained and used for the study. Based on the previously reported genetic map, four additional SSR markers RM3330, RM564, RM3628 and RM6298 were added to the interval harboring Pi25 on rice chromosome 6. Relied on the marker genotyping of RM3330 and A7, 109 Pi25 homozygous lines were selected from the six RIL populations. Markerassisted selection analysis was carried out on the acquired 109 Pi25 homozygous lines using existing markers linked to fertility restoring genes. As a result, twenty restorer lines resistant to blast were obtained. Studies on fertility restoring ability of the 20 restorer lines concluded that the seedsetting rate of crosses between these restorer lines and cytoplasmic male sterility lines (Zhong 9A and Ⅱ32A) reached a normal level. In addition, three populations were selected to be artificially inoculated with a regionally prevalent isolate 05201 of Magnaporthe grisea to evaluate the accuracy of markerassisted selection. The result manifested that simultaneous selection of the markers flanking the gene on both sides could increase the selection accuracy significantly.
ZHOU Haipeng,#,ZHAN Xiaodeng et al. Breeding of Rice Restorer Lines Carrying Blast Resistance Gene Pi25 by MarkerAssisted Selection[J]. , 2008, 22(6): 590-590～596 .
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