中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

水稻立枯丝核菌G蛋白β亚基基因的克隆、表达及序列分析

肖勇1 ;李双成1;初明光1 ;周鹏1;关鹏1;柳莉1;王玲霞1;郑爱萍2,*;李平1,*   

  1. 1四川农业大学 水稻研究所， 四川 温江 611130； 2四川省农业生物技术工程研究中心， 四川 温江 611130； *通讯联系人， E-mail： liping@cngk.com； aipingsau@hotmail.com
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-09-10 发布日期:2008-09-10

Cloning,Expression and Characterization of G Protein β-subunit Gene in Rhizoctonia solani from Rice

XIAO Yong; LI Shuang-cheng; CHU Ming-guang; ZHOU Peng; GUAN Peng; LIU Li; WANG Ling-xia; ZHENG Ai-ping; LI Ping   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-10 Published:2008-09-10

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被引次数

24

摘要： 根据Thanatephorus cucumeris G蛋白β亚基序列（AY884129）设计引物，对水稻立枯丝核菌AG1IA的 G蛋白β亚基基因进行了克隆。PCR结果得到1条约为1.9 kb的扩增片段，包含1个约1.7 kb的完整开放阅读框，编码366个氨基酸。同源性检索发现该序列与大量Ｇ蛋白β亚基基因明显同源，一致性介于57.34%~88.14%。根据其推导cDNA序列设计引物进行RTPCR分析，发现该基因在对数生长期表达量最高，提示水稻立枯丝核菌AG1IA G蛋白β亚基基因可能具有时空表达特性。

关键词: 水稻, 立枯丝核菌, G蛋白β亚基基因

Abstract: A G protein β-subunit gene in Rhizoctonia solani AG-1IA of rice which causes rice sheath blight disease was isolated. A 1958-bp PCR fragment was obtained by using the specific primers designed from the conserved region in G protein β-subunit homologues from Thanatephorus cucumeris. Amino acid sequence analysis showed that the sequence involved 366 deduced amino acid,encoded a putative peptide which shared significant identity (57.34%-88.14%) with G protein β-subunit homologues from other species. The full-length of the PCR fragment was 1.9 kb with a 1.7-kb open reading frame of the G protein β-subunit gene in R.solani AG-1IA. The results of RT-PCR showed that the G protein β-subunit gene transcript was greatly expressed in all growth period especially at the exponential phase,which indicated that the expression of the G protein β-subunit gene in R.solani AG-1IA might be regulated temporally and spatially.

Key words: rice, Rhizoctonia solani, G protein β-subunit gene, cloning, gene expression