中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻二化螟诱导表达基因OsSABATH启动子的克隆与缺失体构建

李冉#; Sumera AFSHEEN#;周国鑫;任 楠; 娄永根*   

  1. 浙江大学 昆虫科学研究所， 浙江 杭州 310029； #共同第一作者；*通讯联系人, E-mail: yglou@zju.edu.cn
  • 收稿日期:2008-01-25 修回日期:2008-04-11 出版日期:2008-09-10 发布日期:2008-09-10

Cloning of the Promoter Sequence of Rice OsSABATH Gene Specifically Expressed by Chilo suppressalis Infestation and Construction of Its 5' Deletions

LI Ran; Sumera AFSHEEN; ZHOU Guo-xin; REN Nan; LOU Yong-gen*

  

  1. Instite of Insect Sciences, Zhejiang University, Hangzhou 310029, China

  • Received:2008-01-25 Revised:2008-04-11 Online:2008-09-10 Published:2008-09-10
  • Contact: LOU Yong-gen

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被引次数

1

摘要： Northern杂交结果表明，OsSABATH基因受二化螟(Chilo suppressalis)为害诱导表达，但却不受机械损伤诱导。利用该基因序列和水稻基因组数据库相应序列设计引物，扩增到该基因编码区5′上游2050 bp的启动子序列。用PLACE软件分析顺式作用元件，发现该基因起始密码子上游1.4 kb的区域内存在几个可能与该基因表达调控相关的顺式作用元件，如W盒、DOF 结合序列、GT1、 BIHD1 结合序列等。将该基因启动子区域及其5′部分缺失构件与GUS报告基因相连，并克隆进pCAMBIA1391载体中，用于分析启动子活性及虫伤诱导特异性相关的顺式作用元件。

关键词: 水稻, 二化螟, 启动子, 顺式作用元件, 克隆

Abstract: Northern blot analysis showed that the expression levels of the rice gene OsSABATH were up-regulated by infestation of rice striped stem borer,Chilo suppressalis,but not by mechanical wounding. A 2050-bp promoter sequence upstream of this gene was amplified using a pair of primers designed on the basis of the sequence of the OsSABATH and rice genome sequence database. With the aid of PLACE program,several cis-acting elements in the promoter sequence,including W-box,DOF binding motif,GT-1 element,and BIHD1 binding motif,were recognized. For analyzing the activity of the promoter sequence and the functions of the herbivore feeding responsive cis-acting elements,the promoter region sequence and its 5'deletion constructs were fused to the GUS reporter gene in pCAMBIA1391 vector.

Key words: rice, Chilo suppressalis, promoter, cis-acting element, cloning