中国水稻科学

• 研究简报 • 上一篇    下一篇

利用回交重组自交系定位稻米赖氨酸含量的基因座位

唐绍清1, 2 ;胡培松2 ;罗 炬2 ;焦桂爱2 ;万建民3 ;石春海1,*   

  1. 1浙江大学 农业与生物技术学院 农学系, 浙江 杭州 310029; 2中国水稻研究所 水稻生物学国家重点实验室, 浙江 杭州 310006; 3南京农业大学 农学院, 江苏 南京 210095; *通讯联系人, E-ail: chhshi@zju.edu.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-10 发布日期:2008-07-10

QTLs Mapping for Lysine Content of Rice Using Backcross Inbred Lines

TANG Shao-qing; HU Pei-song; LUO Ju; JIAO Gui-ai; WAN Jian-min; SHI Chun-hai*   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-10 Published:2008-07-10

摘要: 利用日本晴(粳稻)/Kasalath(籼稻)//日本晴组合衍生的98个回交重组自交系(BILs)株系和具有245个RFLP标记的遗传图谱,在浙江和海南2个环境条件下,开展了精米赖氨酸含量的QTL定位。精米赖氨酸含量在浙江和海南均表现连续变异和超亲分离。在第6染色体发现2个具有显著遗传主效应的QTL(qLYS61和qLYS62),表型贡献率分别为2708%和4756%。qLYS61 还具有显著的环境互作效应。qLYS61的增效基因来自Kasalath,而qLYS62的增效基因来自日本晴。未检测到显著的上位性效应。

关键词: 水稻, 回交重组自交系, 赖氨酸含量, 数量性状座位, 遗传主效应, 基因型×环境互作效应

Abstract: A backcross inbred lines (BILs) population consisting of 98 lines derived from a cross of Nipponbare (japonica)/Kasalath (indica) Nipponbare was used to detect QTLs controlling the lysine content of milled rice in two distinct environments, Zhejiang and Hainan Provinces, China. The lysine content of BILs ranged from 2.19 mg/g to 4.03 mg/g in Zhejiang and 2.39 mg/g to 4.29 mg/g in Hainan, with continuous variation and transgressive segregation. Two putative QTLs (qLYS6-1 and qLYS6-2) with genetic main effects were detected on chromosome 6, which contributed to 27.08 % and 47.56% of the total phenotypic variations, respectively. qLYS6-1 was also detected to have significant genotype X environment interaction effects. The additive effect of qLYS6-1 came from Kasalath, while qLYS6-2 from Nipponbare. In addition, no additive additive epistasis for QTLs was detected.

Key words: rice, backcross inbred lines, lysine content, quantitative trait locus, genetic main effect, genotype×environment interaction effect