中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

拟南芥热激启动子（HSP18.2）在水稻中的启动效率探讨

文春描1,2;徐正君1,*;蔡平钟2,*;向跃武2;张志雄2;刘俊2;王闵霞2;蒲志刚2;张志勇2   

  1. 1 四川农业大学 水稻研究所, 四川 成都 611130； 2 四川省农业科学院 生物技术核技术研究所, 四川 成都 610066； *通讯联系人, E-mail: caipzhong@126.com
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-05-10 发布日期:2008-05-10

Efficiency of Heat-Inducible Promoter (HSP18.2) from Arabidopsis thaliana in Rice

WEN Chun-miao , XU Zheng-jun* , CAI Ping-zhong , XIANG Yue-wu , ZHANG Zhi-xiong , LIU Jun , WANG Min-xia , PU Zhi-gang , ZHANG Zhi-yong   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10

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摘要： 从拟南芥中克隆热激蛋白基因HSP18.2的启动子区域，构建了由HSP18.2启动子引导的GUS融合基因，并经农杆菌介导导入籼稻愈伤组织中。对热激和常温条件下转基因籼稻愈伤组织中GUS活性的比较测定表明，HSP18.2启动子在热激条件下可驱动GUS报告基因在转基因愈伤组织中高效表达，而常温条件下GUS活性在检测水平以下，证明HSP18.2启动子在籼稻中对基因的调控十分灵敏。

关键词: 水稻, 基因条件表达, 热激处理, β葡萄糖醛酸苷酶, 启动子

Abstract: In order to use heat-inducible promoter in transgenic rice research,the 5′-upstream regulation region of 18.2 kD heat-shock protein gene(HSP18.2) was cloned from Arabidopsis thaliana.The cloned HSP18.2 promoter was fused to the 5′-upstream of GUS(beta-glucuronidase) coding region in a binary vector,and introduced into indica rice by Agrobacterium-mediated transformation.The result of histochemical staining analysis of GUS activity under both heat-shock treatment and normal conditions showed that the expression level of GUS fusion gene was significantly upregulated in transformed indica rice calli under heat shock treatment,suggesting that the HSP18.2 promoter could trigger the expression of foreign genes with high efficiency in transgenic indica rice calli.

Key words: Oryza sativa, conditional gene expression, heat-shock, β-glucuronidase, promoter