中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

水稻细菌性条斑病非寄主抗性基因Rxo1的双右边界双元载体的构建

许美容1;夏志辉2;翟文学2 ;徐建龙1;周永力1，* ;黎志康1   

  1. 1中国农业科学院 作物科学研究所/农作物基因资源与基因改良国家重大科学工程， 北京 100081; 2中国科学院 遗传与发育生物学研究所， 北京100101； *通讯联系人， E-mail: zhouyl@caas.net.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-03-10 发布日期:2008-03-10

Construction of Double Right-Border Binary Vector Carrying Non-Host Gene Rxo1 Resistant to Rice Bacterial Leaf Streak

XU Mei-rong , XIA Zhi-hui , ZHAI Wen-xue , XU Jian-long , ZHOU Yong-li , LI Zhi-kang   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-03-10 Published:2008-03-10
  • Contact: ZHOU Yong-li

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摘要： Rxo1 是从玉米中克隆的水稻细菌性条斑病非寄主抗性基因。采用ScaⅠ和NgoMⅣ酶切携带Rxo1 基因的载体pCAMBIA13051，HpaⅠ和XmaⅠ酶切双右边界双元载体pMNDRBBin6，通过平端和黏性末端连接目的基因和载体，得到了重组载体pMNDRBBin6Rxo1。 PCR和限制性内切酶酶切以及序列测定证实Rxo1 整合到了双右边界载体pMNDRBBin6中。该载体为利用转基因技术获得无选择标记抗细菌性条斑病水稻育种材料提供了条件。

关键词: Rxo1 基因, 双右边界双元载体, 水稻, 细菌性条斑病

Abstract: Rxo1 cloned from maize was a non-host gene resistant to bacterial leaf streak of rice.pCAMBIA1305-1 withRxo1 was digested withScaⅠandNgoM Ⅳ and the double right-border binary vector pMNDRBBin6 was digested withHpaⅠandXmaⅠ.pMNDRBBin6 carrying the geneRxo1 was acquired by ligation of blunt-end and cohesive end.The results of PCR,restriction digestion and sequencing showed that theRxo1 gene has been cloned into pMNDRBBin6.This double right-border binary vector,being named as pMNDRBBin6-Rxo1,would play a role in breeding marker-free plants resistant to rice bacterial leaf streak by genetic transformation.

Key words: Rxo1 gene, double right-border binary vector, rice, bacterial leaf streak, marker-free