分子标记辅助选择改良水稻光温敏核不育系华201S的白叶枯病抗性

doi:10.3969/j.issn.1001-7216.2011.02.008

中国水稻科学

分子标记辅助选择改良水稻光温敏核不育系华201S的白叶枯病抗性

兰艳荣^#; 王俊义^#; 王弋; 牟同敏^*

(华中农业大学作物遗传改良国家重点实验室/国家植物基因研究中心/国家作物分子育种中心, 湖北武汉 430070; #共同第一作者; *通讯联系人, E-mail: tongmin58@mail.hzau.edu.cn）

收稿日期:2010-07-17 修回日期:2010-09-18 出版日期:2011-03-11 发布日期:2011-03-11

Improvement of Rice Bacterial Blight Resistance of Hua 201S, an Elite Photo-Thermo- Sensitive Genic Male Sterile Line, by Molecular Marker-Assisted Selection

LAN Yan-rong#; WANG Jun-yi#; WANG Yi; MOU Tong-min*

(National Key Laboratory of Crop Genetic Improvement/National Center of Plant Gene Research/National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: tongmin58@mail.hzau.edu.cn)

Received:2010-07-17 Revised:2010-09-18 Online:2011-03-11 Published:2011-03-11

摘要/Abstract

摘要： 以华201S为受体亲本，以含Xa21和Xa7基因的抗白叶枯病材料华恢20为供体亲本，进行1次杂交、3次回交和3次以上自交，每个世代用基因内标记PTA248对Xa21进行分子检测，用连锁标记STSP3（与Xa7的遗传距离为0.9 cM）对Xa7进行检测。新选育的4个株系YR7009(Xa21)、YR7014(Xa21)、YR7016(Xa7)和YR7023(Xa7/Xa21)在生育期、主要农艺性状、育性转换特性方面都与华201S基本相似。YR7009(Xa21)和YR7014(Xa21)对PXO61、PXO99、ZHE173和GD1358等4个菌株具有良好的抗性，而对FuJ和YN24不具有抗性。YR7016(Xa7)和YR7023(Xa7/Xa21)对6个菌株都表现良好的抗性，尤其是聚合双基因的YR7023(Xa7/Xa21)对6个菌株都表现高抗。表明已经将广谱白叶枯病抗性基因Xa21和Xa7渗入到华201S中，成功地改良了华201S的白叶枯病抗性。

关键词: 水稻, 光温敏核不育系, 分子标记辅助选择, 白叶枯病, 抗性改良

Abstract: Hua 201S is an elite photo-thermo-sensitive genic male sterile(PTGMS) line, which was used for developing two-line hybrid rice. A bacterial blight resistant breeding line ‘Huahui 20’ containing Xa21 and Xa7 genes as donor parent was crossed with Hua 201S. F₁was backcrossed to Hua 201S for three times. The BC₃F₁ progenies were advanced to BC₃F₃ by selfing. The primers PTA248 for Xa21 and STSP3 (genetic distance to Xa7 is 0.9 cM) for Xa7 were used for molecular marker-assisted selection in each progeny. The fertility alterations, main agronomic traits and growth durations of the four selected lines YR7009(Xa21), YR7014(Xa21), YR7016(Xa7) and YR7023(Xa7/Xa21) were similar to the recipient parent Hua 201S. YR7009(Xa21) and YR7014(Xa21) were resistant against four Xanthomonas oryzae pv. oryzae（Xoo） strains, PXO61,PXO99, ZHE173 and GD1358, but not resistant against two Chinese strains, FuJ and YN24. YR7016(Xa7) and YR7023(Xa7/Xa21) were resistant against all the six Xoo strains tested. It showed that Xa21 and Xa7 were introgressed into the PTGMS line Hua 201S, improving the resistance against rice bacterial blight.

Key words: rice, photo-thermo-sensitive genic male sterile line, molecular marker-assisted selection, bacterial blight, resistance improvement, breeding

中图分类号:

兰艳荣, 王俊义, 王弋, 牟同敏 . 分子标记辅助选择改良水稻光温敏核不育系华201S的白叶枯病抗性[J]. 中国水稻科学 2011,25(2): 169-174 . , DOI: 10.3969/j.issn.1001-7216.2011.02.008 .

LAN Yan-rong#,WANG Jun-yi#,WANG Yi,MOU Tong-min* . Improvement of Rice Bacterial Blight Resistance of Hua 201S, an Elite Photo-Thermo- Sensitive Genic Male Sterile Line, by Molecular Marker-Assisted Selection[J]. Chinese Journal of Rice Science 2011,25(2): 169-174 ., DOI: 10.3969/j.issn.1001-7216.2011.02.008 .

参考文献

[1]章琦. 水稻白叶枯病抗性基因鉴定进展及其利用. 中国水稻科学, 2005, 19(5): 453-459.
[2]Shanti M L, George M L C, et al. Identification of resistance genes effective against rice bacterial bight pathogen in Eastern India. Plant Dis, 2001, 85(5): 506-512.
[3]Lee S W, Choi S H, Han S S, et al. Distribution of Xanthomonas oryzae pv. oryzae strains virulent to Xa21 in Korea.Phytopathology, 1999, 89: 928-933.
[4]曾列先, 黄少华, 伍尚忠. IRBB21(Xa21)对广东稻白叶枯病菌5个小种的抗性反应. 植物保护学报, 2002, 29: 97-100.
[5]Singh S, Sidhu J S, Huang N, et al. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106. Theor Appl Genet, 2001,102: 1011-1015.
[6] 邓其明, 周宇爝, 蒋昭雪, 等. 白叶枯病抗性基因Xa21、Xa4和Xa23的聚合及其效应分析. 作物学报, 2005, 31: 1241-1246.
[7]秦钢, 李杨瑞, 李道远. 水稻白叶枯病抗性基因Xa4、Xa23聚合及分子标记检测. 分子植物育种, 2007, 5(5): 625-630.
[8]Perez L M, Redoa D E, Mendioro M S, et al. Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice (Oryza sativa L.) for the development of two-line hybrids. Euphytica, 2008, 164: 627-636.
[9]Gopalakrishnan S, Sharma R K, Anand R K, et al. Integrating marker assisted background analysis with foreground selection for identification of superior bacterial blight resistant recombinants in Basmati rice. Plant Breeding, 2008, 127: 131-139.
[10]罗彦长, 吴爽, 王守海, 等. 聚合抗白叶枯病双基因三系不育系R106A的选育研究. 中国农业科学, 2005, 38(11): 2157-2164.
[11]Mou T M, Lu X G, Hoan N T, et al. Two-line hybrid rice breeding in and outside China//Virmani S S, Mao C X, Hardy B. Hybrid Rice for Food Security, Poverty Alleviation, and Environmental Protection. Fourth International Symposium on Hybrid Rice, Vietnam, May 2002. Manila: International Rice Research Institute, 2002: 31-52.
[12]牟同敏, 李春海. 中籼型光温敏核不育系华201S的选育与应用研究.西南农业学报, 2005, 18(增): 36-40.
[13]牟同敏, 李春海. 两系杂交中稻新组合华两优1206. 杂交水稻, 2006, 21(1): 91-92.
[14]Miyao A, Zhong H S, Monna L, et al. Characterization and genetic mapping of simple sequence repeats in the rice genome. DNA Res, 1996, 3(4): 233-238.
[15]方中达, 许志刚, 过崇俭, 等. 中国水稻白叶枯病菌致病菌型的研究. 植物病理学报, 1990, 20(2): 81-88.
[16]Mew T W. Current status and future prospects of research on bacterial blight of rice. Annu Rev Phytopathol, 1987, 25: 359-382.
[17]Liu H, Wang Y, Hu B, et al. Virulence analysis and race classification of Xanthomonas oryzae pv. oryzae in China. J Phytopathol, 2007, 155(3): 129-135.
[18]Kauffman H E, Reddy A P K, Hsieh S P Y, et al. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep, 1973, 57: 537-541.
[19]Chen S, Lin X H, Xu C G, et al. Improvement of bacterial blight of Minghui 63, an elite restorer line of hybrid rice, by molecular marker-assisted selection. Crop Sci, 2000, 40: 239-244.

分子标记辅助选择改良水稻光温敏核不育系华201S的白叶枯病抗性

Improvement of Rice Bacterial Blight Resistance of Hua 201S, an Elite Photo-Thermo- Sensitive Genic Male Sterile Line, by Molecular Marker-Assisted Selection

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	任志奇, 薛可欣, 董铮, 李小湘, 黎用朝, 郭玉静, 刘文强, 郭梁, 盛新年, 刘之熙, 潘孝武. 水稻外卷叶突变体ocl1的鉴定及基因定位[J]. 中国水稻科学, 2023, 37(4): 337-346.
[2]	肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A/bar*基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358.
[3]	李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367.
[4]	汪胜勇, 陈宇航, 陈会丽, 黄钰杰, 张啸天, 丁双成, 王宏伟. 水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响[J]. 中国水稻科学, 2023, 37(4): 368-378.
[5]	董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404.
[6]	韩聪, 何禹畅, 吴丽娟, 郏丽丽, 王磊, 鄂志国. 水稻碱性亮氨酸拉链(bZIP)蛋白家族功能研究进展[J]. 中国水稻科学, 2023, 37(4): 436-448.
[7]	沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232.
[8]	段敏, 谢留杰, 高秀莹, 唐海娟, 黄善军, 潘晓飚. 利用CRISPR/Cas9技术创制广亲和水稻温敏雄性不育系[J]. 中国水稻科学, 2023, 37(3): 233-243.
[9]	程玲, 黄福钢, 邱一埔, 王心怡, 舒宛, 邱永福, 李发活. 籼稻材料570011抗褐飞虱基因的遗传分析及鉴定[J]. 中国水稻科学, 2023, 37(3): 244-252.
[10]	王文婷, 马佳颖, 李光彦, 符卫蒙, 李沪波, 林洁, 陈婷婷, 奉保华, 陶龙兴, 符冠富, 秦叶波. 高温下不同施肥量对水稻产量品质形成的影响及其与能量代谢的关系分析[J]. 中国水稻科学, 2023, 37(3): 253-264.
[11]	刘嫒桦, 李小坤. 不同肥料施用与稻米品质关系的整合分析[J]. 中国水稻科学, 2023, 37(3): 276-284.
[12]	杨晓龙, 王彪, 汪本福, 张枝盛, 张作林, 杨蓝天, 程建平, 李阳. 不同水分管理方式对旱直播水稻产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 285-294.
[13]	魏晓东, 宋雪梅, 赵凌, 赵庆勇, 陈涛, 路凯, 朱镇, 黄胜东, 王才林, 张亚东. 硅锌肥及其施用方式对南粳46产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 295-306.
[14]	林聃, 江敏, 苗波, 郭萌, 石春林. 水稻高温热害模型研究及其在福建省的应用[J]. 中国水稻科学, 2023, 37(3): 307-320.
[15]	郑承梅, 孙金秋, 刘梦杰, 杨永杰, 陆永良, 郭怡卿, 唐伟. 水稻田糠稷种子萌发和出苗特性及化学防除药剂筛选[J]. 中国水稻科学, 2023, 37(3): 321-328.