Improvement of Rice Bacterial Blight Resistance of Hua 201S, an Elite Photo-Thermo- Sensitive Genic Male Sterile Line, by Molecular Marker-Assisted Selection
LAN Yan-rong#; WANG Jun-yi#; WANG Yi; MOU Tong-min*
(National Key Laboratory of Crop Genetic Improvement/National Center of Plant Gene Research/National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: email@example.com)
Hua 201S is an elite photo-thermo-sensitive genic male sterile(PTGMS) line, which was used for developing two-line hybrid rice. A bacterial blight resistant breeding line ‘Huahui 20’ containing Xa21 and Xa7 genes as donor parent was crossed with Hua 201S. F1 was backcrossed to Hua 201S for three times. The BC3F1 progenies were advanced to BC3F3 by selfing. The primers PTA248 for Xa21 and STSP3 (genetic distance to Xa7 is 0.9 cM) for Xa7 were used for molecular marker-assisted selection in each progeny. The fertility alterations, main agronomic traits and growth durations of the four selected lines YR7009(Xa21), YR7014(Xa21), YR7016(Xa7) and YR7023(Xa7/Xa21) were similar to the recipient parent Hua 201S. YR7009(Xa21) and YR7014(Xa21) were resistant against four Xanthomonas oryzae pv. oryzae（Xoo） strains, PXO61,PXO99, ZHE173 and GD1358, but not resistant against two Chinese strains, FuJ and YN24. YR7016(Xa7) and YR7023(Xa7/Xa21) were resistant against all the six Xoo strains tested. It showed that Xa21 and Xa7 were introgressed into the PTGMS line Hua 201S, improving the resistance against rice bacterial blight.
LAN Yan-rong#,WANG Jun-yi#,WANG Yi et al. Improvement of Rice Bacterial Blight Resistance of Hua 201S, an Elite Photo-Thermo- Sensitive Genic Male Sterile Line, by Molecular Marker-Assisted Selection[J]. , 2011, 25(2): 169-174 .
章琦. 水稻白叶枯病抗性基因鉴定进展及其利用. 中国水稻科学, 2005, 19(5): 453-459. Shanti M L, George M L C, et al. Identification of resistance genes effective against rice bacterial bight pathogen in Eastern India. Plant Dis, 2001, 85(5): 506-512. Lee S W, Choi S H, Han S S, et al. Distribution of Xanthomonas oryzae pv. oryzae strains virulent to Xa21 in Korea.Phytopathology, 1999, 89: 928-933. 曾列先, 黄少华, 伍尚忠. IRBB21(Xa21)对广东稻白叶枯病菌5个小种的抗性反应. 植物保护学报, 2002, 29: 97-100. Singh S, Sidhu J S, Huang N, et al. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106. Theor Appl Genet, 2001,102: 1011-1015.  邓其明, 周宇爝, 蒋昭雪, 等. 白叶枯病抗性基因Xa21、Xa4和Xa23的聚合及其效应分析. 作物学报, 2005, 31: 1241-1246. 秦钢, 李杨瑞, 李道远. 水稻白叶枯病抗性基因Xa4、Xa23聚合及分子标记检测. 分子植物育种, 2007, 5(5): 625-630. Perez L M, Redoa D E, Mendioro M S, et al. Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice (Oryza sativa L.) for the development of two-line hybrids. Euphytica, 2008, 164: 627-636. Gopalakrishnan S, Sharma R K, Anand R K, et al. Integrating marker assisted background analysis with foreground selection for identification of superior bacterial blight resistant recombinants in Basmati rice. Plant Breeding, 2008, 127: 131-139. 罗彦长, 吴爽, 王守海, 等. 聚合抗白叶枯病双基因三系不育系R106A的选育研究. 中国农业科学, 2005, 38(11): 2157-2164. Mou T M, Lu X G, Hoan N T, et al. Two-line hybrid rice breeding in and outside China//Virmani S S, Mao C X, Hardy B. Hybrid Rice for Food Security, Poverty Alleviation, and Environmental Protection. Fourth International Symposium on Hybrid Rice, Vietnam, May 2002. Manila: International Rice Research Institute, 2002: 31-52. 牟同敏, 李春海. 中籼型光温敏核不育系华201S的选育与应用研究.西南农业学报, 2005, 18(增): 36-40. 牟同敏, 李春海. 两系杂交中稻新组合华两优1206. 杂交水稻, 2006, 21(1): 91-92. Miyao A, Zhong H S, Monna L, et al. Characterization and genetic mapping of simple sequence repeats in the rice genome. DNA Res, 1996, 3(4): 233-238. 方中达, 许志刚, 过崇俭, 等. 中国水稻白叶枯病菌致病菌型的研究. 植物病理学报, 1990, 20(2): 81-88. Mew T W. Current status and future prospects of research on bacterial blight of rice. Annu Rev Phytopathol, 1987, 25: 359-382. Liu H, Wang Y, Hu B, et al. Virulence analysis and race classification of Xanthomonas oryzae pv. oryzae in China. J Phytopathol, 2007, 155(3): 129-135. Kauffman H E, Reddy A P K, Hsieh S P Y, et al. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep, 1973, 57: 537-541. Chen S, Lin X H, Xu C G, et al. Improvement of bacterial blight of Minghui 63, an elite restorer line of hybrid rice, by molecular marker-assisted selection. Crop Sci, 2000, 40: 239-244.