中国水稻科学

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水稻高节位分蘖的QTL定位和互作分析

姜华1,#; 赵江红2,#; 郭龙彪3,* ; 姜亮3; 薛大伟4; 曾大力3; 钱前3; 孙国昌1,*   


  1. 1浙江省农业科学院 植物保护与微生物研究所, 浙江 杭州 310021; 2浙江大学 985农业生物与环境科技创新平台, 浙江 杭州 310029; 3中国水稻研究所 水稻生物学国家重点实验室, 浙江 杭州310006; 4杭州师范大学 生命与环境科学学院, 浙江 杭州 310036;#共同第一作者; *通讯联系人, E-mail: guolongb@mail.hz.zj.cn; sungc@zaas.org)
  • 收稿日期:2010-04-06 修回日期:2010-04-16 出版日期:2011-03-11 发布日期:2011-03-11

QTL Mapping and Epistatic Analysis of High-Order Tillering in Rice (Oryza sativa)

JIANG Hua 1,#, ZHAO Jiang-hong 2,#, GUO Long-biao 3,* , JIANG Liang 3, XUE Da-wei4, ZENG Da-li3, QIAN Qian3, SUN Guo-chang 1,*
  

  1. 1Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2985-Institute of Agrobiology and Environmental Science, Zhejiang University, Hangzhou 310029, China; 3State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;4College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China;#These authors contributed equally to this paper; *Corresponding author, E-mail: guolongb@mail.hz.zj.cn; sungc@zaas.org)
  • Received:2010-04-06 Revised:2010-04-16 Online:2011-03-11 Published:2011-03-11

摘要: 高节位分蘖是水稻生产中常见的现象,同时高节位分蘖与水稻的驯化也存在密切的联系。利用来源于窄叶青8号与京系17及春江06与台中本地1号的两个加倍单倍体(DH)群体(分别简称为ZJDH群体和TCDH群体)为材料,对水稻高节位分蘖的遗传特征进行了研究。采用复合区间作图法,在ZJDH群体中共定位到qHOT3、qHOT6-1和qHOT8等3个QTL,分别位于第3、6和8染色体上;对TCDH群体的QTL定位共检测到相关位点2个,分别位于第6和12染色体上。同时,在两个群体中分别检测到4对和7对上位性互作位点。QTL比较分析表明在两个群体中分别定位到的第6染色体上的QTL所在区间可能一致,说明水稻第6染色体对高节位分蘖具有重要的影响。

关键词: 水稻, 高节位分蘖, 加倍单倍体群体, 数量性状基因座

Abstract: High-order tillering (HOT) is a common phenomenon in rice production, which is an important syndrome of rice domestication. Two different doubled haploid populations, one derived from a cross between Jingxi 17 and Zhaiyeqing 8 and the other from a cross between Chunjiang 06 and TN1 (herein designated as ZJDH and TCDH, respectively) were used to study genetic characteristics of high-order tillering. By using composite interval mapping, three QTLs (qHOT3, qHOT6-1 and qHOT8) located on chromosomes 3, 6 and 8 in ZJDH population, and two QTLs on chromosomes 6 and 12 in TCDH population, respectively were detected. Meanwhile, four and seven pairs of epistatic loci affecting HOT were mapped in ZJDH and TCDH, respectively. Furthermore, comparative analysis of two QTLs qHOT6-1 and qHOT6-2, which were located on chromosome 6 in the two populations, respectively indicated that the related quantitative trait loci may be identical to each other. And chromosome 6 may play an important role in high-order tillering.

Key words: rice (Oryza sativa), high-order tillering, doubled haploid population, quantitative trait locus

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