镍处理下水稻酚酸和酚多聚物含量的变化与白叶枯病诱导抗性的关系

doi:10.3969/j.issn.1001-7216.2010.04.018

摘要/Abstract

摘要： 研究了镍处理下水稻叶片中总酚、酚酸、酚多聚物积累和细胞壁结合态过氧化物酶（POD）活性的变化。镍处理导致总酚含量显著上升，检测到10种可溶性酚酸和7种结合态酚酸。镍处理下可溶性绿原酸和水杨酸含量明显上升，而它们的结合态含量变化不明显；镍处理显著提高可溶性和结合态阿魏酸的含量，显著降低可溶性丁香酸和对香豆酸的含量，却诱导其结合态含量增加。镍处理后酚多聚物明显积累，以阿魏酸和丁香醛为底物的细胞壁结合态POD的活性显著升高。这些结果结合以前的研究提示，酚酸和酚多聚物积累参与了镍诱导的水稻对白叶枯病的抗性。

关键词: 酚酸, 酚多聚物, 诱导抗性, 白叶枯病, 镍, 水稻

Abstract: Changes in accumulation of total phenolics, phenolic acids, phenolic polymers and activity of cell wall bound peroxidase (POD) were investigated in leaves of rice exposed to nickel treatment. Ten soluble phenolic acids and seven bound phenolic acids were detected. The levels of soluble chlorogenic acid and salicylic acid were significantly promoted by nickel treatment, whereas the contents of their bound forms remained unchanged. Nickel treatment evidently increased the concentrations of both soluble and bound ferulic acid. However, nickel treatment led to a significant decrease in the contents of soluble syringic acid and pcoumaric acid but an increase in those of their bound forms. Moreover, the pronounced accumulation of phenolic polymers and enhancement in activities of cell wall bound POD, either with ferulic acid or syringaldazine as its substrate, were registered in rice leaves following nickel treatment. The present results combined with the previous study suggest that the accumulation of phenolic acids and phenolic polymers are involved in the establishment of nickelinduced resistance against bacterial blight in rice.

Key words: phenolic acids, phenolic polymers, induced resistance, bacterial blight, nickel, rice (Oryza sativa)

谭新中,彭喜旭,胡耀军,唐新科,周平兰,邓小波,王海华. 镍处理下水稻酚酸和酚多聚物含量的变化与白叶枯病诱导抗性的关系[J]. 中国水稻科学 2010,24(4): 438-442 . , DOI: 10.3969/j.issn.1001-7216.2010.04.018 .

TAN Xin-zhong,PENG Xi-xu,HU Yao-jun,TANG Xin-ke,ZHOU Ping-lan,DENG Xiao-bo,WANG Hai-hua*. Relations Between Changes in Contents of Phenolic Acids and Phenolic Polymers and Induced Resistance to Bacterial Blight in Rice under Nickel Treatment[J]. Chinese Journal of Rice Science 2010,24(4): 438-442 ., DOI: 10.3969/j.issn.1001-7216.2010.04.018 .

参考文献

[1]Nicholson R L, Hammerschmidt R. Phenolic compounds and their role in disease resistance. Annu Rev Phytopathol, 1992, 30: 369-389.

[2]Zhao J, Davis L C, Verpoorte R. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv, 2005, 23: 283-333.

[3]Boudet A M, Lapierre C, Grima-Pettenati J. Biochemistry and molecular biology of lignification. New Phytol, 1995, 129: 203-236.

[4]Polle A, Otter T, Seifert F. Apoplastic peroxidases and lignification in needles of Norway spruce (Picea abies L.). Plant Physiol, 1994, 106: 53-60.

[5]Prats E, Bazzalo M E, Léon A, et al. Accumulation of soluble phenolic compounds in sunflower capitula correlates with resistance to Sclerotinia sclerotiorum. Euphytica, 2003, 132: 321-329.

[6]Kováik J, Bakor M. Phenylalanine ammonia-lyase and phenolic compounds in chamomile tolerance to cadmium and copper excess. Water Air Soil Pollut, 2007, 185: 185-193.

[7]Kováik J, Grúz J, Bakor M, et al. Phenolic compounds composition and physiological attributes of Matricaria chamomilla grown in copper excess. Environ Exp Bot, 2008, 62: 145-152.

[8]Tolrà R P, Poschenrieder C, Luppi B, et al. Aluminium-induced changes in the profiles of both organic acids and phenolic substances underlie Al tolerance in Rumex acetosa L. Environ Exp Bot, 2005, 54: 231-238.

[9] 王海华，汪琼，肖用森，等. 硝酸镍诱导水稻白叶枯病抗性与防卫反应酶活性的变化. 杂交水稻, 2003, 18(6): 47-50.

[10]Singleton V L, Orthofer R, Lamuela-Raventos R M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol, 1999, 299: 152-178.

[11]Butsat S, Weerapreeyakul N, Siriamornpun S. Changes in phenolic acids and antioxidant activity in Thai rice husk at five growth stages during grain development. J Agric Food Chem, 2009, 57(11): 4566-4571.

[12]Müsel G, Bergfeld R, Ruel K, et al. Structure and distribution of lignin in primary and secondary cell walls of maize coleoptiles analyzed by chemical and immunological probes. Planta, 1997, 201: 146-159.

[13]Snchez M, Pea M J, Revilla G, et al. Changes in dehydrodiferulic acids and peroxidase activity against ferulic acid associated with cell walls during growth of Pinus pinasfer hypocotyl. Plant Physiol, 1996, 111: 941-946.

[14]Grison R, Pile P M. Properties of syringaldazine oxidase/peroxidase in maize roots. J Plant Physiol, 1985, 118: 201-208.

[15]Sahoo M R, Kole P C, Dasgupta M, et al. Changes in phenolics, polyphenol oxidase and its isoenzyme patterns in relation to resistance in taro against Phytophthora colocasiae. J Phytopathology, 2009, 157: 145-153.

[16]Mikuli Petkovek M, Usenik V, tampar F. The role of chlorogenic acid in the resistance of apples to apple scab (Venturia inaequalis (Cooke) G. Wind. Aderh.). Zb Bioteh Fak Univ Ljublj Kmet, 2003, 81: 233-242.

[17] 李云锋, 王振中, 贾显禄. 稻瘟菌激发子CSBⅠ诱导水稻防御性相关酶的活性变化. 作物学报, 2004, 30(6): 613-617.

[18]Niggeweg R, Michael A J, Martin C. Engineering plants with increased levels of the antioxidant chlorogenic acid. Nat Biotechnol, 2004, 22: 746-754.

[19]Bily A C, Reid L M, Taylor J H, et al. Dehydromers of ferulic acid in maize pericarp and aleurone: Resistance factors to Fusarium gramineum. Phytopathology, 2003, 93: 712-719.

[20]Sarma B K, Singh U P. Ferulic acid may prevent infection of Cicer arietinum by Sclerotium rolfsii. World J Microbiol Biotechnol, 2003, 19: 123-127.

[21]Wang H, Feng T, Peng X, et al. Up-regulation of chloroplastic antioxidant capacity is involved in alleviation of nickel toxicity of Zea mays L. by exogenous salicylic acid. Ecotoxicol Environ Safe, 2009, 72: 1354-1362.

[22]Klessig D F, Malamy J. The salicylic acid signal in plants. Plant Mol Biol, 1994, 26: 1439-1451.

[23]Horváth E, Szalai G, Janda T. Induction of abiotic stress tolerance by salicylic acid signaling. J Plant Growth Regul, 2007, 26: 290-300.

[24]王海华, 曹赐生, 康健, 等. 水杨酸诱导的水稻对白叶枯病的系统抗性与未处理叶酶活性的变化. 中国水稻科学, 2002, 16(3): 252-256.

[25] 王海华，曾富华，康健, 等. 镍处理对水稻叶片H2O2积累和抗病的诱导效应．植物生理学报, 2001, 27(1): 61-65.

[26]Goldberg R, Catesson A M, Czaninski Y. Some properties of syringaldazine oxidase, a peroxidase specifically involved in lignification process. Z Pflanzenphysiol, 1983, 110: 267-279.

[27]Reimers P J, Leach J E. Race-specific resistance to Xanthomonas oryzae pv. oryzae conferred by bacterial blight resistance gene Xa-10 in rice (Oryza sativa) involves accumulation of a lignin-like substance in host tissues. Physiol Mol Plant Pathol, 1991, 38: 39-55.

[28]Reimers P J, Guo A, Leach J E. Increased activity of a cationic peroxidase associated with an incompatible interaction between Xanthomonas oryzae pv. oryzae and rice (Oryza sativa). Plant Physiol, 1992, 99: 1044-1050.

[29]Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish J Environ Stud, 2006, 15(4): 523-530.

[30]Sgherri C, Cosi E, Navari-Izzo F. Phenols and antioxidative status of Raphanus sativus grown in copper excess. Physiol Plant, 2003, 118: 21-28.