The effect of H2O2 pretreatment on Cd tolerance and translocation in rice seedlings was studied by using two rice cultivars (N076 and N0763) differing in Cd tolerance. The contents of malondialdehyde(MDA), reduced glutathione (GSH), nonprotein thiols (NPT), phytochelatins(PCs) and the activity of glutathione Stransferase (GST) were compared between the two cultivars exposed to various treatments. The results showed that 50 μmol/L Cd exposure significantly inhibited rice growth, enhanced the production of GSH, NPT, PCs, MDA and the activity of GST, and there were significant differences between the two cultivars. More Cd was transported into the shoot in N076. The H2O2 pretreatment alleviated Cd toxcity by further increasing GSH, NPT and PCs contents, as well as the root GST activities. The larger increase in N0763 than in N076 suggests that the tolerance of N0763 was enhanced more significantly than N076. H2O2 pretreatment reduced Cd translocation to rice shoots but affected the Cd concentration in roots differently. From the above results, it may be speculated that there were clear differences in the Cd detoxification and response to the H2O2 pretreatment between the two cultivars.
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