Pyramiding of Translucent Endosperm Mutant Gene Wx-mq and Rice Stripe Disease Resistance Gene Stv-bi by MarkerAssisted Selection in Rice (Oryza sativa)
YAO Shu1,2 , CHEN Tao1, ZHANG Ya-dong1, ZHU Zhen1, ZHAO Ling1, ZHAO Qing-yong1, ZHOU Li-hui1, WANG Cai-lin1，*
1Institute of Food Crops， Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R & D Center/Nanjing Branch of Chinese National Center for Rice Improvement， Nanjing 210014, China; 2 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China; *Corresponding author, E-mail: firstname.lastname@example.org
A high yielding japonica rice variety, Wuyunjing 7, bred in Jiangsu Province, China as female parent was crossed with the Japanese rice variety Kanto 194, which carries the rice stripe disease resistance gene Stvbi and translucent endosperm mutant gene Wxmq. From F2 generations, a SCAR (sequence characterized amplified region) marker tightly linked with Stvbi and a CAPS (cleaved amplified polymorphic sequence) marker for Wxmq were used for markerassisted selection. Finally, a new japonica rice line, Ning 9108, with excellent agronomic traits was bred by multigenerational selection on stripe disease resistance and endosperm appearance. The utilization of the markers from genes related to rice quality and disease resistance was helpful not only for establishing a molecular markerassisted selection system of highquality and disease resistance in rice, but also for providing important intermediate materials and rapid selection method for good quality, disease resistance and high yield in rice breeding.
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