Genetic Analysis and Mapping of a ThermoSensitive White Stripe Leaf Mutant at the Seedling Stage in Rice(Oryza sativa)
LI Chao1, LIN Dong-zhi1,*, DONG Yan-jun1, *, YE Sheng-hai2, ZHANG Xiao-ming2
1Laboratory of Plant Genetics and Functional Genes, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China; 2Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; *Corresponding author, E-mail: firstname.lastname@example.org; email@example.com
A thermosensitive white stripeleaf mutant (tws) was selected from the M2 progeny of a japonica rice variety, Jiahua 1, treated with 60Co γradiation. In comparison with the wild type parent (Jiahua 1), the mutant displayed a phenotype of white stripe on the 3rd and 4th leaves, but began to turn a normal green phenotype on the 5th leaf when grown at low temperatures (20℃, 24℃). And also the content of total chlorophyll showed an obvious decrease in the leaves with white stripe. These results suggest that the expression of the mutant trait was thermosensitive and correlated with the leaf age of seedlings. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene, designated as tws (thermosensitive white stripeleaf). In addition, by using SSR markers and an F2 segregating population derived from the cross between the tws mutant and 9311, the tws gene was mapped between MM3907 and MM3928 with a physical distance of 86 kb on chromosome 4.
LI Chao,LIN Dong-zhi,DONG Yan-jun et al. Genetic Analysis and Mapping of a ThermoSensitive White Stripe Leaf Mutant at the Seedling Stage in Rice(Oryza sativa) [J]. , 2010, 24(3): 223-227 .
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