中国水稻科学

中国水稻科学

• 研究报告 •    下一篇

应用剩余杂合体衍生的近等基因系分解水稻产量性状QTL

杜景红;樊叶杨;王磊;庄杰云*   

  1. 中国水稻研究所， 浙江 杭州 310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-01-10 发布日期:2008-01-10

Dissection of QTLs for Yield Traits by Using Near Isogenic Lines Derived from Residual Heterozygous Lines in Rice

DU Jing-hong , FAN Ye-yang , WANG Lei , ZHUANG Jie-yun   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
  • Contact: ZHUANG Jie-yun

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被引次数

33

摘要： 以杂合区间为RM587-RM402的水稻剩余杂合体（RHL）衍生群体为材料，应用SSR标记检测，筛选到杂合区间分别为RM587-RM225、RM204-RM6119和RM6119-RM402的3个单株，进一步检测其F2群体，分别获得母本纯合型材料10株、父本纯合型材料10株和杂合型材料20株。种植这3套近等基因系材料，考查单株产量及其构成因子每株穗数、每穗实粒数和千粒重。经应用目标区间内等位基因效应分析和交迭重组染色体片段代换系分析，分解出3个控制每穗实粒数的QTL和2个控制单株产量的QTL，这些QTL分别位于物理距离为0.66 ~ 2.49 Mb的区间中，全部表现为加性作用为主，增效等位基因除qNFGP61来自父本密阳46外，其余均来自母本珍汕97B。提出了构建新型遗传材料，提高水稻QTL精细定位效率的策略。

关键词: 剩余杂合体, 近等基因系, 数量性状座位, 产量性状, 水稻

Abstract: Three residual heterozygous lines(RHLs)carrying heterozygous segments in intervals RM587-RM225,RM204-RM6119 and RM6119-RM402 on the short arm of rice chromosome 6,respectively,were selected from a rice population derived from an RHL for interval RM587-RM402.Ten maternal homozygotes,10 paternal homozygotes and 20 heterozygotes were selected from each of the F2 populations derived from the three RHLs.The three sets of near isogenic lines(NILs)were grown for phenotyping on grain yield per plant,number of panicles per plant,number of filled grains per panicle and 1000-grain weight.With analysis on the phenotype differences among the three genotype groups in each NIL set and that among overlapping chromosome segment substitution lines,three QTLs for filled grain number per panicle and two QTLs for grain yield were resolved.They were located in intervals ranging from 0.66 Mb to 2.49 Mb.The additive effect was higher than the dominance effect at each locus.The allele for increasing the trait value was derived from the paternal parent at qNFGP6-1,and from the maternal parent at the other QTLs.Following the present study,an approach for the construction of new genetic resource to facilitate QTL fine-mapping in rice was proposed.

Key words: residual heterozygous line, near isogenic line, quantitative trait locus, yield traits, rice