中国水稻科学

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盐胁迫下水稻苗期Na+含量的QTL定位

汪斌1; 兰涛2, 3 ; 吴为人2, 3,*   

  1. 1福建农林大学 生命科学学院, 福建 福州 350002; 2福建农林大学 作物科学学院, 福建 福州 350002; 3福建省水稻分子标记辅助育种重点实验室, 福建 福州 350002;
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-11-10 发布日期:2007-11-10

Mapping of QTLs for Na+ Content in Rice Seedlings under Salt Stress

WANG Bin , LAN Tao , WU Wei-ren   

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-11-10 Published:2007-11-10
  • Contact: WU Wei-ren

摘要: 利用来自籼稻品种H359和Acc 8558的一个重组自交系群体(131个株系)及相应的遗传图谱(包含147个RFLP和79个SSR标记),以150 mmol/L的NaCl处理后的幼苗地上部Na+含量为指标,采用复合区间定位方法,对水稻耐盐性QTL进行了定位。共检测到13个QTL,分别位于第1、2、5、6、7和12染色体上,对表型变异的总贡献率达到60.88%,其中,qSC1b的效应最大,可解释约45%的表型变异。通过比较表明,许多前人报道的与水稻盐胁迫有关的QTL/基因与本研究检测到的QTL的位置相同或相近。

关键词: 水稻, 钠离子含量, 数量性状座位, 盐胁迫, 耐盐性

Abstract: Based on a recombinant inbred population consisting of 131 lines derived from a cross between two indica rice varieties,H359 and Acc 8558,and a corresponding genetic map comprising 147 RFLP and 79 SSR markers,QTL mapping was performed for salt tolerance in rice indicated by Na+ content in shoots of seedlings after treatment with 150 mmol/L NaCl by using the method of composite interval mapping.A total of 13 QTLs were detected on chromosomes 1,2,5,6,7 and 12,which could explain 60.88% of total phenotypic variance.Among the QTLs,qSC1b had the largest additive effect,explaining about 45% of the phenotypic variance.The QTLs identified in this study shared the same or similar regions with many salt-stress-related QTLs/genes reported previously.

Key words: rice, sodium ion content, quantitative trait loci, salt stress, salt tolerance