中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

基因枪转化的bar基因表达盒在水稻中的重组结构

赵艳1;钱前2，*;王慧中3;高振宇2; 颜美仙2;黄大年2   

  1. 1 浙江工商大学 食品生物与环境工程学院， 浙江 杭州 310035; 2 中国水稻研究所 水稻生物学国家重点实验室， 浙江 杭州 310006; 3 杭州师范大学 生命科学学院， 浙江 杭州 310036; *通讯联系人, E-mail:qianqian188@163.com
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-10 发布日期:2007-09-10

Recombination Structure of bar Gene Expression Cassette in Rice Transformed by Particle Bombardment

ZHAO Yan , QIAN Qian , WANG Hui-zhong , GAO Zhen-yu , YAN Mei-xian , HUANG Da-nian   

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-10 Published:2007-09-10
  • Contact: QIAN Qian

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被引次数

18

摘要： 应用Southern杂交和引物组合PCR技术研究了基因枪转化的bar基因表达盒（包括启动子、编码区和终止子）在水稻（Oryza sativa L.）中的重组结构。bar基因表达盒的多个拷贝通过重组连接形成1～3个转基因串联子,彼此邻近整合在受体基因组内一个较大的染色体区域，不同转基因串联子之间由水稻基因组DNA间隔，形成转基因簇。bar基因表达盒形成转基因串联子时,存在头接头、头接尾、尾接尾3种连接方式，通常伴随着bar基因片段的截短。

关键词: bar基因表达盒, 基因枪法转化, 转基因串联子, 重组结构, 水稻

Abstract: The recombination structure of bar gene cassette(containing the promoter,coding region and terminator) in rice(Oryza sativa L.) genome transformed by particle bombardment was studied by Southern blotting and primer combination PCR technology.Multiple copies of bar gene cassette were recombined and linked to 1-3 transgene arrays in rice genome.Different transgene arrays were insulated by rice genome DNA and tended to integrate in a large area of rice chromosome,lying in nearby loci and forming transgene clusters.There were three kinds of link forms among bar gene fragments including head to head,head to tail and tail to tail when forming transgene arrays,always accompanied with truncation.

Key words: bar gene cassette, transformation by particle bombardment, transgene array, recombination structure, rice