中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

野生稻酯酶等电聚焦电泳分析

黄大年; 赵式英; 王金霞; 范在丰   

  1. 中国水稻研究所
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1988-08-20 发布日期:1988-08-20

The Analysis on the Esterase Isozymes of Wild Rice Species by Isoelectric Focusing Electrophoresis

Huang Danian; Zhao Shiying; Wang Jinxia; Fan Zaifeng   

  • Received:1900-01-01 Revised:1900-01-01 Online:1988-08-20 Published:1988-08-20

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被引次数

7

摘要： 对16个野生稻种(基因组分别为AA、BB、CC、CCDD、BBCC、EE和FF)的酯酶等电聚焦电泳分析表明:普通野生稻组的普通野生稻系和宽叶野生稻系、狭叶野生稻组的酯酶酶谱各有其特定模式,这和传统的稻属分类结果相一致。同种野生稻不同地理分布地收集的样品酯酶酶谱模式基本相同,但有些物种有差异。酯酶酶谱可以作为野生稻分类的依据之一,又能揭示同一野生稻种内细微遗传差异。

关键词: 基因组, 野生稻, 酯酶, 等电聚焦

Abstract: The 16 given wild rice species belong to the genomes AA, BB, CC, CCDD, BBCC, EE, or FF. The results of esterase isozymes isoelectric focusing electrophoresis indicate that there was individually a special pattern of esterase among Ser. sativae, Ser. latifoliae and Sect. Angustifolia of Oryza. The taxonomic results by analyzing esterase pattern for above three wild rice species groups were similar to that by classic taxonomy of wild rice species. And the samples of same species, collected from different geographical regions, mostly share similar pattern of esterase. However, there were variances in some esterase isozymes bands varied with species. Therefore, the esterase patterns can be used as one of the criteria of classification for wild rice species, and meanwhile used to reveal the fine genetic diversity in the same species as well.

Key words: Genome, Wild rice, Esterase, Isoelectric focusing