中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

应用微卫星标记评估我国水稻主栽品种的一致性

应杰政1，2;施勇烽2;庄杰云2;薛庆中1，*   

  1. 1 浙江大学 农业与生物技术学院 农学系， 浙江 杭州 310029； 2 中国水稻研究所 国家水稻改良中心/水稻生物学国家重点实验室， 浙江 杭州 310006； *通讯联系人， E-mail: qzhxue@hotmail.com
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-07-10 发布日期:2006-07-10

Assessment of Uniformity of Major Rice Varieties in China Using Microsatellite Markers

YING Jie-zheng , SHI Yong-feng , ZHUANG Jie-yun , XUE Qing-zhong   

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-10 Published:2006-07-10

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被引次数

31

摘要： 为较准确地评估我国水稻主栽品种的一致性，挑选５个微卫星标记，对４１个有2~7个来源的水稻品种进行了微卫星标记检测。结果表明，１８个（４３．９０％）品种存在同一品种名称不同来源间的差异。进一步选择１１个代表性水稻品种或杂交稻组合作较大样本分析（５０个单株），它们在５个微卫星标记上的不一致率为０．０％～１０．０％，平均为１．６７％，每个微卫星标记的异质率变化幅度为０．３６％～５．４５％。１１个品种中有６个（５４．５５％）在所有位点上完全一致，不一致率通常都不超过５％ 。因此，在构建水稻品种微卫星数据库时，必须慎重选择取样来源。当鉴别特定材料时，则应相应增加标记数。

关键词: 水稻, 微卫星标记, 一致性, 品种保护, 品种鉴定

Abstract: To have a better assessment of the within-variation variation of the major rice varieties in China,41 varieties from two to seven sources were assayed with a set of five well-chosen microsatellite markers,18 varieties(43.90%) of which showed heterogeneity among the different sources with the same name at 1 to 4 loci.Eleven varieties were further selected and analyzed with larger samples containing 50 individuals per variety.Non-uniformity of the varieties varies from 0.0-10.0% with an overall average of 1.67% on average five SSR loci,and the heterogeneity of the markers ranges from 0.36% to 5.45%.It also showed that 6 of the 11 typical rice varieties were completely uniform,and the non-uniformities within varieties across all loci were lower than 5%.Therefore,it is advisable that the sources of the materials should be selected carefully in constructing a microsatellite database,and that the number of markers should be correspondingly increased when identifying some specific materials.

Key words: rice, simple sequence repeat, uniformity, variety protection, variety identification