中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

籼稻杂交亲本与其F1代胚乳蛋白最高营养pI范围内组分之量变及种植季节对其影响

李 毓1;刘奕田 2; 伍时照 3   

  1. 1 福建农业大学 作物遗传育种研究所,福建 福州 350002; 2 华南农业大学 生物技术学院,广东 广州 510642;3华南农业大学 农学系，广东 广州 510642；*通讯联系人
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-07-10 发布日期:1999-07-10

Quantitative Change of Components in the Best Nutrient pI Range of Endosperm Proteins in Indica Rice Crossing Parents and Their F1 Generation and the Influence of Cropping Seasons on It

Li Yu; Liu Yitian; Wu Shizhao   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-07-10 Published:1999-07-10

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摘要： 以华南地区7个早籼品种及其所配12对正反交组合F1代为材料,在对它们胚乳全蛋白进行等电聚焦聚丙烯酰胺凝胶电泳(Isoelectrofocusing polyacrylamide gel electrophoresis,IEF-PAGE) 分离定量基础上,确定最高营养pI (等电点)范围蛋白组分之比率;用统计学方法分析其变动与杂交方式及种植季节的关系。结果表明,无论所试材料遗传背景如何,它们的IEF-PAGE图谱均具有十分相似的特征;最高营养pI范围组分含量比率,正交与反交无显著差异，而早晚季间差异显著。

关键词: 籼稻, 胚乳, 蛋白组分, 种植季节, 杂交

Abstract: Using seven early indica rice cu1tivars cultivated in South China and the twelve pairs of reciprocal combinations produced by them as materials, the contents (ratios ) of the protein components of the best nutrient pI range were determined,/ based on the separation of entire endosperm proteins through isoelectrofocusing-polyacrylamlde gel electrophoresis（IEF-PAGE） and the quantitative analysis by chromato automatic scan. The relationship between the quantitative change and crossing ways, and the cropping seasons was analyzed by statistics.The results revealed that no matteter under whatever genetic background, the IEF-PAGE spectrums of all tested materials and the scanning spectrums took on extremely similar features in the contents (ratios) of the best nutrient pI range components, There was no significant difference between reciprocal crosses. but there was between the early and late seasons in the protein contents of the best nutrient pI range.

Key words: indica rice, endosperm, protein composition, cropping seasons, crossing