中国水稻科学

中国水稻科学

• 研究报告 •    下一篇

受GA3诱导的一个水稻伸长相关基因的初步鉴定

莫肖蓉; 董海涛; 吴玉良; 何祖华; 李德葆   

  1. 浙江大学华家池校区生物技术研究所, 浙江杭州 310029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-01-10 发布日期:2000-01-10

Isolation and Cloning of a Candidate Elongation Related Gene in Rice Induced by GA3

Mo Xiaorong; Dong Haitao; Wu Yuliang; He Zuhua; Li Debao   

  • Received:1900-01-01 Revised:1900-01-01 Online:2000-01-10 Published:2000-01-10

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摘要： 采用mRNA 差异显示技术对受GA3 诱导的水稻伸长相关基因进行了分离和鉴定。经100 个引物组合差异显示,在获得21 个受GA3 诱导表达的cDNA 片段的基础上, 对其中的GA 15b 片段克隆进行了序列测定。其序列经国际联网BLAST 查询表明GA 15b 为伸展蛋白类似蛋白的候选基因片段。定量PCR 进一步确证GA 15b 受GA3 所诱导。Southern 杂交表明GA 15b 以多拷贝形式存在。

关键词: 水稻, 赤霉素, 伸展蛋白, 基因

Abstract: Gene expression patterns in rice elongation induced by GA3 were investigated by using mRNA differential display technique. From 100 combinations of anchor and arbitrary primers, twenty one GA3 induced cDNA fragments were obtained. One of them was sequenced and shown to encode extensin like protein. Quantitative PCR confirmed that the expression of GA15b was induced by GA3. Southern blot analysis indicated that the GA15b was a multicopy gene.

Key words: Oryza sativa, gibberellin, extensin like protein, gene