中国水稻科学

中国水稻科学

• 实验技术 • 上一篇    下一篇

水稻SSR不同检测和分析方法的比较研究

李 莉1;杨剑波1;D. J. Mackill2;P. M. Colowit2   

  1. 1安徽省农业科学院 水稻遗传育种农业部重点开放实验室，安徽 合肥 230031; 2 美国加州大学　戴维斯学院　水稻遗传学实验室，美国 加州 95616
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-07-10 发布日期:2000-07-10

Comparison of Different Detection Methods for Rice SSR Analysis

LI Li 1; YANG Jian-bo 1; D.J.MACKILL 2; P.M.COLOWIT 2   

  1. (1Key Laboratory of Rice Genetics and Breeding for Agricultural Ministry of China; Anhui Academy of Agricultural Sciences; Hefei 230031; China; 2 Rice Genetics Laboratory; University of California-Davis, CA95616, USA)
  • Received:1900-01-01 Revised:1900-01-01 Online:2000-07-10 Published:2000-07-10

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被引次数

86

摘要： 用15对水稻微卫星引物，对13个不同类型及来源的水稻基因型材料进行了简单序列重复片段长度的多态性(SSLPs)分析，并与不同的检测和分析方法进行了比较。聚丙烯酰胺凝胶电泳无论是放射性自显影分析还是荧光标记的自动测序仪分析都比高密度的琼脂糖电泳(3% MetaPhor agarose)有效。利用荧光标记自动测序系统的SSLPs分析较之传统的32P标记的聚丙烯酰胺凝胶电泳分析，具有以下优点：(1)借助于荧光染料标记的丰富内标，可以区别几个bp之差的SSLPs ；(2)借助于不同的荧光标记可以在同一样孔里分析多个样品；(3)自动给出SSLPs片段的长度；(4)自动的数据输出和分析；(5)避免了放射性同位素有可能造成的环境污染。因此，该方法具有快速、高效的特点。

关键词: 水稻, 简单序列重复, 多态性检测

Abstract: By using 15 microsatellite DNA primers, Simple Sequence Length Polymorphism(SSLPs) of 13 rice genotypes were analyzed and detected with different methods. The results show that denaturing polyacrylamide electrophoresis including isotopic labelling and automated fluorescence is more effective than electrophoresis on 3% MetaPhor agarose gel . The advantage of the automated fluorescence system as compared to standard [ 32P] labelling sequencing gels are: (1) several bases resolving clearly ; (2) the ability to visualize and analyze by several products in the same lane within the same size range, but with different fluorescent labels; (3)automated sizing; (4)automated data output; and (5)elimination of radioactivity. These advantages increase the efficiency and rapidity of rice SSLPs analysis.

Key words: rice, simple sequence repeat, polymorphic detection, research method