中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

转抗菌肽B基因和bar基因籼稻植株的再生

王慧中1，2; 华志华2;高振宇2;颜美仙2;钱 前2;黄大年2   

  1. 1杭州师范学院　生物系，浙江 杭州 310012; 2中国水稻研究所，浙江 杭州 310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-07-10 发布日期:2000-07-10

Regeneration of Transgenic Indica Rice Plants with a Cecropin B Gene and a bar Gene

WANG Hui-zhong 1; 2; HUA Zhi-hua 2; GAO Zhen-yu 2; YAN Mei-xian 2; QIAN Qian 2; HUANG Da-nian   

  1. (1Department of Biology; Hangzhou Normal College; Hangzhou 310012; China; 2China National Rice Research Institute; Hangzhou 310006; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2000-07-10 Published:2000-07-10

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摘要： 运用基因枪法将含有bar基因和cecropin B基因的质粒pCB1导入籼稻品种特青的幼胚细胞，筛选后得到3株转基因植株。PCR检测和Southern杂交结果表明，外源基因已整合到水稻基因组中。转化当代植株表现出对Basta很强的抗性，同时也增强了对水稻白叶枯病的抗性。

关键词: 水稻, bar基因, 抗菌肽B基因, 转基因植株, 抗病性, 白叶枯病

Abstract: Immature embryos of indica rice variety Teqing were bombarded with the plasmid pCB1 containing a bar gene and a cecropin B gene. Three transgenic plants were regenerated after selection. The results of PCR assay and Southern blot analysis showed that foreign genes had been integrated into the rice genome. Transformed plants showed high resistance to herbicide Basta and Xanthomonas campestris pv. oryzae.

Key words: rice, bar gene, cecropin B gene, transgenic plant, disease resistance, Xanthomonas campestris pv.oryzae