中国水稻科学

• 研究简报 • 上一篇    

淹水胁迫下水稻根尖细胞中Ca2+和Ca2+-ATP酶的分布

李阳生1; 王建波2   

  1. 1中国科学院 长沙农业现代化研究所,湖南 长沙 410125;E-mail:ysli@ms.csiam.ac.cn; 2 武汉大学 生命科学学院,湖北 武汉 430072
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-07-10 发布日期:2001-07-10

Distribution of Ca2+ and Ca2+-ATPase in Root Apical Cells of Rice under Submergence Stress

LI Yang-sheng 1; WANG Jian-bo 2   

  1. (1 Changsha Institute of Agricultural Modernization; Chinese Academy of Sciences; Changsha 410125; China; 2College of Life Sciences; Wuhan University; Wuhan 430072; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-07-10 Published:2001-07-10

摘要: 应用电镜细胞化学方法,对淹水胁迫下水稻根尖细胞中Ca2+和Ca2+-ATP酶分布的变化进行了观察。正常生长条件下,Ca2+主要分布于液泡和细胞间隙中,而细胞质基质、细胞核中分布很少;Ca2+-ATP酶主要分布于质膜上,线粒体膜、内质网膜、核膜上也有酶分布。经过没顶淹水处理后,液泡、细胞间隙中的Ca2+沉淀颗粒明显减少,而胞质及核基质中的沉淀颗粒明显增多,同时,质膜及其他膜结构上Ca2+-ATP酶的活性明显降低。淹水造成根尖细胞中Ca2+-ATP酶活性降低,胞质和核基质中Ca2+积累,将影响细胞的正常生理代谢活动。

关键词: 水稻, 根尖细胞, 淹水胁迫, 钙离子, 超微结构

Abstract: Changes of Ca2+ and Ca2+-ATPase distribution in root apical cells of rice under submergence stress were determined by cytochemical methods. Grown under normal environment, Ca 2+ mainly distributed in vacuoles and intercellular space, and there was little Ca2+ in matrix of cytoplasm and nucleus. Moreover, Ca2+-ATPase was demonstrated on plasma membrane, as well as on membrane of endoplasmic reticulum, mitochondria and nucleus. Alternatively, the distribution of Ca2+ and Ca2+-ATPase changed obviously under submergence stress. The Ca2+ in vacuoles and intercellular space decreased and it deposited in matrix of cytoplasm and nucleus. On the other hand, the viability of Ca2+-ATPase on plasma membrane and other membrane decreased. This distributing change of Ca2+ and Ca2+-ATPase could be one of the reasons that induce roots to be destroyed under submergence stress.

Key words: rice, root apical cell, submergence stress, calcium ion, ultrastructure