中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

水稻胡麻斑类似病危害转Bt稻“克螟稻”初报

唐 健1; 叶恭银2; 杨保军1; 赵新华1; 蒋跃南1; 舒庆尧3; 袁筱萍1   

  1. 1中国水稻研究所， 浙江 杭州 310006; E-mail:tangjian@mail.hz.zj.cn; 2[KG*2]浙江大学 植物保护系, 浙江 杭州 310029； 3 浙江大学 原子核农业研究所， 浙江 杭州 310029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-10-10 发布日期:2001-10-10

Preliminary Study on Rice Brown Spot Mimic Lesion Virulence on Bt Gene Transformed Rice Kemingdao

TANG Jian 1; YE Gong-yin 2; YANG Bao-jun 1; ZHAO Xin-hua 1; JIANG Yue-nan 1; SHU Qing-yao 3; YUAN Xiao-ping 1   

  1. (1China National Rice Research Institute; Hangzhou 310006; China; E-mail: tangjian@mail.hz.zj.cn; 2Plant Protection Department, Zhejiang University, Hangzhou 310029, China; 3 Institute of Nuclear Agricultural Sciences, Zhejiang Unversity, Hangzhou 310029, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-10-10 Published:2001-10-10

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摘要： 于田间转Bt稻“克螟稻”稻叶上观测到一种病斑，病斑中心和周围均呈褐色，长椭圆形与稻胡麻斑病类似，平板上分离到的病原菌菌丝呈灰褐色，菌丝有多个隔膜，分生孢子梗成丛，但在PDA培养基上，产生的分生孢子特征与稻胡麻斑病不吻合。从为害级别和严重度上分析克螟稻对胡麻斑类似病的感病程度远远超过其亲本秀水11。克螟稻叶片间发病速率增长迅速，而秀水11叶片间发病速率增长缓慢。

关键词: 苏云金杆菌基因, 转基因, 水稻, 胡麻斑类似病, 危害性

Abstract: A diseased spot had been observed on leaves of Bt gene transformed rice Kemingdao in paddy field. The lesion was similar to the rice brown spot, however, the fungus had not been identified as Cochiliobolus miyabeanus according to the characteristics of spores produced on PDA media. Three indices, i.e. severity, maximum injure degree and injure degree were used to evaluate the virulence of rice brown spot mimic lesion on Bt gene transformed rice Kemingdao 1 (KMD 1), Kemingdao 2 (KMD 2), as well as their original parent Xiushui 11. The results indicated that KMD was much more susceptible than Xiushu 11. Comparison with the virulence among top leaf to the 5th leaf, a significant difference indicated that the lesion occurred sequentially from the bottom to the top, and damaged KMD more seriously than their parent Xiushui 11.

Key words: Bt gene, gene transfer, Oryza sativa, rice brown spot mimic lesion, virulence