中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

不同水稻品种种子耐超干性差异及其热稳定蛋白的研究

朱 诚1; 刘 信1; 曾广文1; 景新明2; 吴 平1   

  1. 1 浙江大学 生命科学学院， 浙江 杭州 310029； E-mail:zhucheng@mail.hz.zj.cn; 2 中国科学院 植物研究所， 北京100093
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-10-10 发布日期:2001-10-10

Difference of Ultra-drying Tolerance and Heat-stable Proteins in Seeds of Different Rice Cultivars

ZHU Cheng 1; LIU Xin 1; ZENG Guang-wen 1; JING Xing-ming 2; WU Ping 1   

  1. (1 College of Life Science; Zhejiang University; Hangzhou 310029; China; E-mail:zhucheng@mail.hz.zj.cn; 2 Beijing Botanical Garden; Chinese Academy of Sciences; Beijing 100093, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-10-10 Published:2001-10-10

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摘要： 以不同水稻品种（籼稻、粳稻、杂交稻等）为材料，研究了品种之间种子耐超干能力的差异及其与热稳定蛋白的关系。结果表明，供试的粳稻品种（春江15和春江683）不耐超干，当含水量降低至4．5％以下，种子发芽率和活力指数极显著下降，而籼稻品种（J106和J174）耐超干能力强，含水量降低至3．5％以下对种子发芽率和活力指数没有影响，杂交稻（汕优63）和中香糯介于两者之间。耐超干的品种存在一特异性的热稳定蛋白条带，其分子量为36.3 kD，而不耐超干的品种种子无此条带，这表明水稻的耐超干性与该热稳定蛋白有关

关键词: 水稻, 种子, 耐超干性, 热稳定蛋白

Abstract: The difference of ultra drying tolerance and the heat stable proteins were studied among different rice cultivars, and two ideas inferred from the results. (1) The germination rate and vigor index of japonica rice seeds (Chunjiang 15 and Chunjiang 683) decreased rapidly when their moisture content fell to 4.5%. On the contrary, the germination rate and vigor index of indica rice (J174 and J106) seeds changed little even when their moisture content fell to 3.5%, which indicated that indica rice had strong ultra-drying tolerance. While the seeds' tolerance of waxy rice and hybrid rice (Shanyou 63) were intervened in japonica rice’s and indica rice's. (2) SDS-PAGE electrophoresis showed that a 36.3 kD heat stable protein appeared only in seeds of rice cultivars (indica rice, waxy rice and hybrid rice) with ultra drying tolerance. These results suggested that the special heat stable proteins might be related to ultra drying tolerance.

Key words: Oryza sativa, seed, ultra trying tolerance, heat stable protein