聚合稻瘟病抗性基因Pigm-1和Pid2的水稻三系不育系福梦A的选育与利用

doi:10.16819/j.1001-7216.2026.241209

摘要/Abstract

摘要：

【目的】水稻稻瘟病是水稻生产上最主要的病害之一，对全球水稻生产构成严重威胁。大规模和长期种植具有单一抗性基因的水稻品种通常会导致抗性的丧失。通过分子标记辅助选择技术，选育抗病品种是防治稻瘟病最有效的方法之一。【方法】首先，以泰丰B为受体，分别以携带Pigm-1的双抗77009和携带Pid2的地谷B为供体，通过杂交、回交和复交的方法，并借助于分子标记辅助育种技术，对各世代单株基因型进行分析与鉴定。其次，利用不同来源的12个稻瘟病菌和田间自然鉴定的方法对选育的福梦A及组合进行抗性鉴定。最后，通过田间种植与调查，对不育系福梦A的育性、主要农艺性状以及配合力与杂种优势进行系统的分析，并对其抑制恢复系感光的特性进行了分析。【结果】培育一个同时含有稻瘟病抗性基因Pigm-1和Pid2新的三系不育系福梦A，室内与田间接菌显示该不育系抗稻瘟病；育性鉴定结果显示福梦A不育株率和不育度均为100%，柱头外露率达57.39%；遗传背景与农艺性状分析显示福梦A遗传了轮回亲本的主要农艺性状，具有更好的丰产性；杂种优势分析显示，福梦A配制组合其产量有一半高于对照天优华占，具有较好的生产应用潜力。【结论】通过分子标记辅助育种技术，育成1个高抗稻瘟病、不育性稳定、柱头外露率高、农艺性状和产量性状优良的新三系不育系福梦A(B)，实现了不育系稻瘟病抗性的定向改良，为三系杂交水稻育种应用创制了新的亲本材料。

关键词: 水稻, 抗稻瘟病, Pigm-1, 三系不育系, 聚合育种, 抑制感光

Abstract:

【Objective】Rice blast is one of the most important diseases in rice production and poses a serious threat to global rice production. The large-scale and long-term planting of varieties with a single resistance gene often leads to the loss of resistance. Molecular marker-assisted selection is one of the most effective approaches for controlling rice blast..【Method】Using Taifeng B as the recipient, and Shuangkang 77009 carrying Pigm-1 and Digu B carrying Pid2 as donors, respectively. The genotypes of each generation were analyzed and identified by means of hybridization, backcrossing, backcrossing and recurred cross, aided by molecular marker-assisted breeding. Subsequently, the blast resistance of the developed sterile line Fumeng A and its hybrids was evaluated using 12 blast isolates from diverse sources under natural field conditions. Finally, through field trials, the fertility, main agronomic traits, combining ability, and heterosis of Fumeng A were systematically analyzed, and the photo-sensitive characteristics of its corresponding restorer line were also examined.【Result】A new three-line male sterile line, Fumeng A, carrying both Pigm-1 and Pid2, was developed. Inoculation tests both in the laboratory and field confirmed its blast resistance. Fertility identification showed that both the sterility rate and sterility degree of Fumeng A reached 100%, with a stigma exsertion rate of 57.39%. Genetic background and agronomic trait analysis indicated that Fumeng A inherited the main agronomic traits of the recurrent parent and exhibited improved yield potential. Heterosis analysis revealed that half of the hybrid combinations derived from Fumeng A yielded higher than the control variety Tianyouhuazhan, demonstrating good potential for production application.【Conclusion】Using molecular marker-assisted selection, a novel three-line male sterile line, Fumeng A (B), with high blast resistance, stable sterility, high stigma exsertion, and excellent agronomic and yield traits was developed. This work achieved targeted improvement of blast resistance in a sterile line and created a new parental material for three-line hybrid rice breeding.

Key words: rice, resistance to rice blast, Pigm-1, three-line sterile line, polymerization breeding, photoinhibition

程朝平, 何旎清, 白康呈, 林少俊, 黄凤凰, 刘军化, 程祖锌, 黄成志, 杨德卫. 聚合稻瘟病抗性基因Pigm-1和Pid2的水稻三系不育系福梦A的选育与利用[J]. 中国水稻科学, 2026, 40(1): 72-84.

CHENG Zhaoping, HE Niqing, BAI Kangcheng, LIN Shaojun, HUANG Fenghuang, LIU Junhua, CHENG Zuxin, HUANG Chengzhi, YANG Dewei. Breeding and Utilization of the Three-Line Male Sterile Line Fumeng A with Pyramided Rice Blast Resistance Genes Pigm-1 and Pid2[J]. Chinese Journal OF Rice Science, 2026, 40(1): 72-84.

图/表 11

参考文献 29

[1]	Skamnioti P, Gurr S J. Against the grain: Safeguarding rice from rice blast disease[J]. Trends in Biotechnology, 2009, 27(3): 141-150.
[2]	Deng Y W, Zhai K R, Xie Z, Yang D Y, Zhu X D, Liu J Z, Wang X, Qin P, Yang Y Z, Zhang G M, Li Q, Zhang J F, Wu S Q, Milazzo J, Mao B Z, Wang E T, Xie H A, Tharreau D, He Z H. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J]. Science, 2017, 355(6328): 962-965.
[3]	谢华安. 杂交水稻抗病虫育种实践与思考[J]. 中国稻米, 2020, 26(1): 1-5.
	Xie H A. Practice and thinking of hybrid rice breeding with disease-resistant and insect-resistance[J]. China Rice, 2020, 26(1): 1-5. (in Chinese with English abstract)
[4]	王如意, 刘杰, 冯琴, 张熠玚, 肖宁, 吴俊, 郑文静, 李爱宏, 宁约瑟. 稻瘟病抗性分子机制及抗病育种策略研究进展[J]. 植物保护, 2023, 49(5): 32-42.
	Wang R Y, Liu J, Feng Q, Zhang Y Y, Xiao N, Wu J, Zheng W J, Li A H, Ning Y S. Deciphering the molecular mechanism of rice blast resistance and research progress of resistance breeding strategy[J]. Plant Protection, 2023, 49(5): 32-42. (in Chinese with English abstract)
[5]	吴俊, 刘雄伦, 戴良英, 王国梁. 水稻广谱抗稻瘟病基因研究进展[J]. 生命科学, 2007, 19(2): 233-238.
	Wu J, Liu X L, Dai L Y, Wang G L. Advances on the identification and characterization of broad-spectrum blast resistance genes in rice[J]. Chinese Bulletin of Life Sciences, 2007, 19(2): 233-238. (in Chinese with English abstract)
[6]	危文亮, 赵应忠. 分子标记在作物育种中的应用[J]. 生物技术通报, 2000(2): 12-16.
	Wei W L, Zhao Y Z. Applications of molecular markers in crop[J]. Biotechnology Bulletin, 2000(2): 12-16. (in Chinese with English abstract)
[7]	孙富, 唐梅, 何聪, 卢宏琮. 分子标记辅助聚合基因Pi1和Pi2改良红色特种籼稻稻瘟病抗性研究[J]. 湖北农业科学, 2018, 57(18): 23-27.
	Sun F, Tang M, He C, Lu H C. Improvement of rice blast resistance in red rice by pyramiding of Pi1 and Pi2 through molecular marker-assisted selection[J]. Hubei Agricultural Sciences, 2018, 57(18): 23-27. (in Chinese with English abstract)
[8]	柳武革, 王丰, 李金华, 朱满山, 廖亦龙, 付崇允, 刘迪林, 曾学勤, 马晓智, 霍兴, 孔乐. 水稻三系不育系的分子设计改良与应用[J]. 广东农业科学, 2021, 48(10): 69-77.
	Liu W G, Wang F, Li J H, Zhu M S, Liao Y L, Fu C Y, Liu D L, Zeng X Q, Ma X Z, Huo X, Kong L. Improvement of three-line rice male sterile lines by molecular design and their application[J]. Guangdong Agricultural Sciences, 2021, 48(10): 69-77. (in Chinese with English abstract)
[9]	陈学伟, 李仕贵, 马玉清, 黎汉云, 周开达, 朱立煌. 水稻抗稻瘟病基因Pi-d(t)¹、Pi-b、Pi-ta²的聚合及分子标记选择[J]. 生物工程学报, 2004, 20(5): 708-714.
	Chen X W, Li S G, Ma Y Q, Li H Y, Zhou K D, Zhu L H. Marker-assisted Selection and Pyramiding for three blast resistance genes, Pi-d(t)¹, Pi-b, Pi-ta², in rice[J]. Chinese Journal of Biotechnology, 2004, 20(5): 708-714. (in Chinese with English abstract)
[10]	楼珏, 杨文清, 李仲惺, 罗天宽, 谢永楚, 郑国楚, 岳高红, 徐建龙, 卢华金. 聚合稻瘟病、白叶枯病和褐飞虱抗性基因的三系恢复系改良效果的评价[J]. 作物学报, 2016, 42(1): 31-42.
	Lou J, Yang W Q, Li Z X, Luo T K, Xie Y C, Zheng G C, Yue G H, Xu J L, Lu H J. Evaluation of improvement effect of restorer lines on pyramiding genes resistant to rice blast, bacterial leaf blight and brown planthopper[J]. Acta Agronomica Sinica, 2016, 42(1): 31-42. (in Chinese with English abstract)
[11]	杨德卫, 何旎清, 黄凤凰. 利用分子标记辅助选择聚合水稻抗病基因Pigm-1和Xa23[J]. 西北农林科技大学学报: 自然科学版, 2023, 51(11): 37-45.
	Yang D W, He N Q, Huang F H. Pyramiding Pigm-1 and Xa23 genes in rice (Oryza sativa L.) by marker-assisted selection[J]. Journal of Northwest A&F University: Natural Science Edition, 2023, 51(11): 37-45. (in Chinese with English abstract)
[12]	陈志伟, 官华忠, 王宗华, 周元昌, 潘润森, 毛大梅, 段远霖, 吴为人. 利用基因聚合技术改良一个优质水稻不育系的稻瘟病抗性[J]. 福建农林大学学报: 自然科学版, 2012, 41(5): 449-455.
	Chen Z W, Guan H Z, Wang Z H, Zhou Y C, Pan R S, Mao D M, Duan Y L, Wu W R. Improvement of the resistance to rice blast in a high quality rice CMS line by gene pyramiding[J]. Journal of Fujian Agriculture and Forestry University: Natural Science Edition, 2012, 41(5): 449-455. (in Chinese with English abstract)
[13]	陈志伟, 官华忠, 毛大梅, 潘润森, 周元昌, 吴为人. 抗稻瘟病和抗白叶枯病两系水稻不育系的选育及其初步应用[J]. 植物遗传资源学报, 2020, 21(5): 1078-1088.
	Chen Z W, Guan H Z, Mao D M, Pan R S, Zhou Y C, Wu W R. Development and preliminary application of a two-line rice male sterile line with resistance to rice blast and bacterial blight[J]. Journal of Plant Genetic Resources, 2020, 21(5): 1078-1088. (in Chinese with English abstract)
[14]	Yang D W, Li S P, Lu L, Fang J B, Wang W, Cui H T, Tang D Z. Identification and application of the Pigm-1 gene in rice disease-resistance breeding[J]. Plant Biology, 2020, 22(6): 1022-1029.
[15]	朱满山, 黄慧君, 王丰, 刘振荣, 柳武革, 廖亦龙, 李金华, 陈建伟. 优质抗稻瘟病弱感光型杂交晚稻新组合泰丰优55[J]. 杂交水稻, 2013, 28(6): 76-77.
	Zhu M S, Huang H J, Wang F, Liu Z R, Liu W G, Liao Y L, Li J H, Chen J W. Taifengyou 55, a new indica hybrid rice combination with weak photosensitivity, fine grain quality and blast resistance[J]. Hybrid Rice, 2013, 28(6): 76-77. (in Chinese with English abstract)
[16]	Li J B, Sun Y D, Liu H, Wang Y Y, Jia Y L, Xu M H. Natural variation of rice blast resistance gene Pi-d2[J]. Genetics and Molecular Research, 2015, 14(1): 1235-1249.
[17]	涂诗航, 周鹏, 郑轶, 张水金, 董瑞霞, 王洪飞, 王晓方, 游晴如, 黄庭旭, 郑家团. 优质不育系泰丰A系列杂交组合的恢复系选育杂交水稻[J]. 杂交水稻, 2018, 33(4): 13-16.
	Tu S H, Zhou P, Zheng Y, Zhang S J, Dong R X, Wang H F, Wang X F, You Q R, Huang T X, Zheng J T. Breeding of restorer lines for high quality CMS line Taifeng A[J]. Hybrid Rice, 2018, 33(4): 13-16. (in Chinese with English abstract)
[18]	高利军, 邓国富, 高汉亮, 高国庆, 周萌, 周维永, 颜群, 张晋, 戴高兴. 水稻抗稻瘟病基因Pi-d2基因标签的建立与应用[J]. 西南农业学报, 2010, 23(1): 77-82.
	Gao L J, Deng G F, Gao H L, Gao G Q, Zhou M, Zhou W Y, Yan Q, Zhang J, Dai G X. Establishment and application of gene tagging linked to rice blast resistance gene Pi-d2[J]. Southwest China Journal of Agriculture Sciences, 2010, 23(1): 77-82. (in Chinese with English abstract)
[19]	Yang D W, Ye X F, Zheng X H, Cheng C P, Ye N, Huang F H. Development and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of the whole wild rice genome[J]. Frontiers in Plant Science, 2016, 7: 1737.
[20]	Yang D W, Li S P, Xiao Y P, Lu L, Zheng Z C, Tang D Z, Cui H T. Transcriptome analysis of rice response to blast fungus identified core genes involved in immunity[J]. Plant, Cell＆Environment, 2021, 44(9): 3103-3121.
[21]	邓一文, 刘裕强, 王静, 陈学伟, 何祖华. 农作物抗病虫研究的战略思考[J]. 中国科学: 生命科学, 2021, 51(10): 1435-1446.
	Deng Y W, Liu Y Q, Wang J, Chen X W, He Z H. Strategic thinking and research on crop disease and pest resistance in China[J]. Scientia Sinica Vitae, 2021, 51(10): 1435-1446. (in Chinese with English abstract)
[22]	刘潮, 韩利红, 褚洪龙, 王海波, 高永, 唐利洲. 植物抗病研究进展与展望[J]. 植物保护, 2018, 44(4): 1-8.
	Liu C, Han L H, Chu H L, Wang H B, Gao Y, Tang L Z. Progresses in and prospects for plant disease resistance research[J]. Plant Protection, 2018, 44(4): 1-8. (in Chinese with English abstract)
[23]	鲁秀梅, 张宁, 陈劲枫, 钱春桃. 作物基因聚合育种的研究进展[J]. 分子植物育种, 2017, 15(4): 1445-1454.
	Lu X M, Zhang N, Chen J F, Qian C T. The research progress in crops pyramiding breeding[J]. Molecular Plant Breeding, 2017, 15(4): 1445-1454. (in Chinese with English abstract)
[24]	Yang D B, Xiong L Z, Mou T M, Mi J M. Improving the resistance of the rice PTGMS line Feng 39S by pyramiding blast, bacterial blight, and brown planthopper resistance genes[J]. The Crop Journal, 2022, 10(4): 1187-1197.
[25]	张世玺, 姚坚, 杨海龙, 王蕾, 李白. 聚合抗稻瘟病基因Pia和抗白叶枯病基因Xa23的广亲和恢复系选育[J]. 江苏农业科学, 2024, 52(11): 111-115.
	Zhang S X, Yao J, Yang H L, Wang L, Li B. The selection of broadly compatible restorer lines with resistance to rice blast disease gene Pia and bacterial blight disease gene Xa23[J]. Jiangsu Agricultural Sciences, 2024, 52(11): 111-115. (in Chinese with English abstract)
[26]	何旎清, 杨德卫, 郑向华, 黄凤凰, 程朝平, 叶宁. 分子标记辅助选择Pigm-1基因改良恢复系R20稻瘟病抗性[J]. 核农学报, 2022, 36(2): 0245-0250.
	He N Q, Yang D W, Zheng X H, Huang F H, Cheng C P, YE N. Improving blast resistance of R20 by molecular marker-assisted selection of Pigm-1 gene[J]. Journal of Nuclear Agricultural Sciences, 2022, 36(2): 0245-0250. (in Chinese with English abstract)
[27]	Gao H, Jin M N, Zheng X M, Chen J, Yuan D Y, Xin Y Y, Wang M Q, Huang D Y, Zhang Z, Zhou K N, Sheng P K, Ma J, Ma W W, Deng H F, Jiang L, Liu S J, Wang H Y, Wu C Y, Yuan L P, Wan J M. Days to heading 7, a major quantitative locus determining photoperiod sensitivity and regional adaptation in rice[J]. Proceedings of the National Academy of Sciences of the United States of America, 2014, 11(46): 16337-42.
[28]	Li X, Zhang R C, Chen G, Xie J X, Xiao Z W, Cao F B, Ali I, Lqbal A, Wahab A, Huang M, Chen J N. Increasing grain weight and yield stability by increasing pre-heading non-structural carbohydrate reserves per spikelet in short-growth duration rice[J]. The Crop Journal, 2023, 11(6): 1912-1920.
[29]	周振玲, 孙志广, 徐卫平, 徐艳, 杨波, 刘艳, 王宝祥, 江玲, 徐大勇. 水稻显性感光抑制基因Su-E1(t)的定位及遗传分析[J]. 植物遗传资源学报, 2020, 21(4): 945-953.
	Zhou Z L, Sun Z G, Xu W P, Xu Y, Yang B, Liu Y, Wang B X, Jiang L, Xu D Y. Genetic mapping of a dominant photosensitive suppresser gene, Su-E1(t) in Oryza sativa[J]. Journal of Plant Genetic Resources, 2020, 21(4): 945-953. (in Chinese with English abstract)

标记 Markers	引物序列 Forward primer (5＇-3＇)	连锁的抗病基因 Target gene	目的片段 Fragment size(bp)
Pigm-1	TTATTTCGTTTGCTATGC(F)	Pigm-1	164, 132
	GGACTATGTGATCGGTTA(R)	Pigm-1	164, 132
M-Pid2	TGTGAAGCAAATGATCACCА(F)	Pid2	1009, 629
	GGCAGTCGTATTGCTGTGAA(R)	Pid2	1009, 629

标记 Markers	引物序列 Forward primer (5＇-3＇)	连锁的抗病基因 Target gene	目的片段 Fragment size(bp)
Pigm-1	TTATTTCGTTTGCTATGC(F)	Pigm-1	164, 132
	GGACTATGTGATCGGTTA(R)	Pigm-1	164, 132
M-Pid2	TGTGAAGCAAATGATCACCА(F)	Pid2	1009, 629
	GGCAGTCGTATTGCTGTGAA(R)	Pid2	1009, 629

稻瘟病菌 Blast fungus	双抗77009 Shuangkang 77009	地谷B Digu B	泰丰A Taifeng A	福梦A Fumeng A	CO39
18SH-D527	R	R	S	R	S
FJ2011	R	R	S	R	S
95085AZB	R	R	−	R	S
18NH-16-3	R	R	S	R	S
FJ81278	R	R	R	R	S
KJ201	R	R	S	R	S
501-3	R	R	S	R	S
RB22	R	R	S	R	S
20-15	R	R	−	R	S
MH86-1	R	R	S	R	S
MH86-3	R	R	S	R	S
M409	R	R	S	R	S

稻瘟病菌 Blast fungus	双抗77009 Shuangkang 77009	地谷B Digu B	泰丰A Taifeng A	福梦A Fumeng A	CO39
18SH-D527	R	R	S	R	S
FJ2011	R	R	S	R	S
95085AZB	R	R	−	R	S
18NH-16-3	R	R	S	R	S
FJ81278	R	R	R	R	S
KJ201	R	R	S	R	S
501-3	R	R	S	R	S
RB22	R	R	S	R	S
20-15	R	R	−	R	S
MH86-1	R	R	S	R	S
MH86-3	R	R	S	R	S
M409	R	R	S	R	S

品系 Line	碘染反应花粉率 I₂-KI staining reaction pollen rate(%)				套袋自交结实率 Percentage of bagged seed setting rate(%)			柱头外露率 Stigma exsertion rate(%)
品系 Line	典败 Typical abortion	圆败 Spherical abortion	染败 Stained abortion	正常可育Fertile	总颖花数Total spikelets	总结实粒数 Filled spikelets	自交结实率 Self- pollinate rate	单边外露 Unilateral	双边外露 Bilateral	总外露 Total
泰丰A Taifeng A	99.68	0.32	0	0	8100	7	0.086	30.06	29.85	59.91
福梦A Fumeng A	99.99	0.01	0	0	9250	0	0	27.67	26.12	53.79
泰丰B Taifeng B	0	0	0	100	9125	8508	93.24	0	0	0
福梦B Fumeng B	0	0	0	100	9832	8907	90.59	0	0	0