超级早稻中早39稻瘟病抗性的系谱分析

doi:10.16819/j.1001-7216.2017.6165 441

摘要/Abstract

摘要：

目的研究常规超级早籼稻品种中早39持久高抗稻瘟病的遗传基础,【方法】利用田间抗性鉴定、稻瘟病抗性基因分子标记检测和基因测序的方法,分析中早39稻瘟病持久抗性的遗传基础。结果中早39、金早47和中组3号对7个稻瘟病抗性基因Piz、Pi9、Pib、Pi36、Pi64、Pi-d2和Pi-d3的分子标记均呈阳性条带;且中早39与金早47、中组3号对5个稻瘟病生理小种的抗性表现完全相同。中早39与金早47、中组3号无论是表型还是基因型都完全一致;序列分析结果表明中早39系谱中的供试材料均含有Pi-d2抗病等位基因。结论中早39的抗稻瘟病特性经中组3号,来源于金早47。含有Pi-d2的地谷与中早39、金早47和中组3号对5个稻瘟病生理小种的表型相同。

关键词: 分子标记, 抗性基因, 基因测序, 系谱分析

Abstract:

【Objective】 To clarify the genetic basis of the durable resistance to Magnaporthe oryzae of the super early rice Zhongzao 39,【Method】blast resistance assessment, molecular marker screening and gene sequencing were conducted.【Result】Zhongzao 39, Jinzao 47 and Zhongzu 3 showed positive bands to all the molecular markers for 7 resistant genes Piz, Pi9, Pib, Pi36, Pi64, Pi-d2 and Pi-d3; The resistance performance of Zhongzao 39, were fully consistent with Jinzao 47 and Zhongzu 3 to the 5 isolates collected from Zhejiang and Jiangxi Province, China; Molecular sequencing results showed that all the test materials contain Pi-d2 resistant alleles. 【Conclusion】These results showed that the genetic basis of durable resistance to Magnaporthe oryzae of Zhongzao 39 was contributed by Jinzao 47 and Zhongzu 3.

Key words: molecular marker, resistant gene, gene sequencing, genealogical analysis

梁燕, 季芝娟, 曾宇翔, 张翠真, 吴晗霖, 杨长登. 超级早稻中早39稻瘟病抗性的系谱分析[J]. 中国水稻科学, 2017, 31(4): 441-446.

Yan LIANG, Zhijuan JI, Yuxiang ZENG, Cuizhen ZHANG, Hanlin WU, Changdeng YANG. Genealogical Analysis on Resistance to Magnaporthe oryzae of Super Early Rice Zhongzao 39[J]. Chinese Journal OF Rice Science, 2017, 31(4): 441-446.

图/表 6

参考文献 16

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名称Name	引物序列 Primer sequence (5′-3′)	目的片段大小Product/bp	目的基因 Target gene	退火温度 Annealing temperature /℃
pid2-F	TTGGCTATCATAGGCGTCC	1057	Pi-d2	55	[13]
pid2-R	ATTTGAAGGCGTTTGCGTAGA
pid3-F	GAATGAGTGGAATCGGCAAG	775	Pi-d3	55	[13]
pid3-R	AATCCTTCAGCAACCCAGAG
pi36-F	CAATGTGTGACTTGTGCGGACT	1036	Pi36	55	[13]
pi36-R	TCTTCCATCTCGGATTTCGTGT
Piz-F	GGACCCGCGTTTTCCACGTGTAA	292	Piz	60	[14]
Piz-R	AGGAATCTATTGCTAAGCATGAC
Pib-F	ATGGAGGATACCGAGTTGACTG	1089	Pib	55	[13]
Pib-R	CCGTTGACTTTGAGATGGCGAT
YRT6-F	TCCTGTGTTTCCTACCGAGTCCAGC	1016	Pi64	55	[15]
YRT6-R	AGAGGAGTGCAAGGTTACCAGAGCC
P9-F	CGGTTATAGATGAATATAGTTC	803	Pi9	50	[16]
P9-R	GTTCGACTCTCCCTTCAAGC
Pid2-2F*	TTGGGTCTAGACCAAACAGAGCTTCTAACAATC	2720	Pi-d2	55	[8]
Pid2-2R*	TTTGCGTCGACCTAGTTACAGATCACTGTGCCAT

名称Name	引物序列 Primer sequence (5′-3′)	目的片段大小Product/bp	目的基因 Target gene	退火温度 Annealing temperature /℃
pid2-F	TTGGCTATCATAGGCGTCC	1057	Pi-d2	55	[13]
pid2-R	ATTTGAAGGCGTTTGCGTAGA
pid3-F	GAATGAGTGGAATCGGCAAG	775	Pi-d3	55	[13]
pid3-R	AATCCTTCAGCAACCCAGAG
pi36-F	CAATGTGTGACTTGTGCGGACT	1036	Pi36	55	[13]
pi36-R	TCTTCCATCTCGGATTTCGTGT
Piz-F	GGACCCGCGTTTTCCACGTGTAA	292	Piz	60	[14]
Piz-R	AGGAATCTATTGCTAAGCATGAC
Pib-F	ATGGAGGATACCGAGTTGACTG	1089	Pib	55	[13]
Pib-R	CCGTTGACTTTGAGATGGCGAT
YRT6-F	TCCTGTGTTTCCTACCGAGTCCAGC	1016	Pi64	55	[15]
YRT6-R	AGAGGAGTGCAAGGTTACCAGAGCC
P9-F	CGGTTATAGATGAATATAGTTC	803	Pi9	50	[16]
P9-R	GTTCGACTCTCCCTTCAAGC
Pid2-2F*	TTGGGTCTAGACCAAACAGAGCTTCTAACAATC	2720	Pi-d2	55	[8]
Pid2-2R*	TTTGCGTCGACCTAGTTACAGATCACTGTGCCAT

菌株名称 Isolate name	抗性 Resistance							种群 Group
菌株名称 Isolate name	特特普 Tetep	珍龙13 Zhenlong 13	四丰43 Sifeng 43	东农363 Dongnong 363	关东51 Guandong 51	合江18 Hejiang 18	丽江新团黑谷 Lijiangxintuanheigu	种群 Group
14-754	抗R	感S	抗R	抗R	抗R	抗R	感S	ZB₃₁
10-105	感S	抗R	抗R	感S	感S	感S	感S	ZA₄₉
12-157	抗R	感S	感S	抗R	抗R	感S	感S	ZB₁₃
16-754	抗R	感S	抗R	抗R	抗R	感S	感S	ZB₂₉
16-161	抗R	抗R	抗R	抗R	抗R	抗R	感S	ZG₁

菌株名称 Isolate name	抗性 Resistance							种群 Group
菌株名称 Isolate name	特特普 Tetep	珍龙13 Zhenlong 13	四丰43 Sifeng 43	东农363 Dongnong 363	关东51 Guandong 51	合江18 Hejiang 18	丽江新团黑谷 Lijiangxintuanheigu	种群 Group
14-754	抗R	感S	抗R	抗R	抗R	抗R	感S	ZB₃₁
10-105	感S	抗R	抗R	感S	感S	感S	感S	ZA₄₉
12-157	抗R	感S	感S	抗R	抗R	感S	感S	ZB₁₃
16-754	抗R	感S	抗R	抗R	抗R	感S	感S	ZB₂₉
16-161	抗R	抗R	抗R	抗R	抗R	抗R	感S	ZG₁

材料 Material	14-754	10-105	12-157	16-754	16-161
浙辐802 Zhefu 802	抗R	抗R	感S	感S	抗R
嘉育293 Jiayu 293	抗R	抗R	感S	感S	抗R
湘早籼1号 Xiangzaoxian 1	抗R	抗R	抗R	抗R	抗R
青谷矮3号 Qinggu'ai 3	抗R	抗R	抗R	抗R	抗R
陆青早1号 Luqingzao 1	抗R	抗R	抗R	抗R	抗R
金早47 Jinzao 47	感S	抗R	抗R	抗R	抗R
中组3号 Zhongzu 3	感S	抗R	抗R	抗R	抗R
嘉育253 Jiayu 253	感S	抗R	抗R	感S	抗R
中早39 Zhongzao 39	感S	抗R	抗R	抗R	抗R
地谷Digu	感S	抗R	抗R	抗R	抗R