黄淮海稻区水稻品种的穗颈瘟抗性

doi:10.16819/j.1001-7216.2019.8104

摘要/Abstract

摘要：

【目的】为明确黄淮海稻区育成水稻品种的稻瘟病抗性及其基因型,【方法】收集了来自本稻区5个不同地区的水稻品种,连续两年通过稻瘟病菌接种鉴定和田间病圃自然诱发对供试品种进行了稻瘟病抗性鉴定,并利用4个抗病基因Pita, Pib, Pi54和Pikm的分子标记,对水稻品种基因型进行了统计分析,并以抗性等级为表型值对品种资源进行聚类分析。【结果】黄淮海稻区水稻品种发病率较高,分别达85.2%和65.9%。Pi54和Pib检出率分别达84%、65%。同时抗性聚类分析表明Pi54和Pib在本稻区的抗性贡献率却很低。【结论】黄淮海稻区水稻品种稻瘟病抗性弱,携带的抗瘟基因的抗性在不断减弱或丧失,需加强稻瘟病主效抗性的发掘、开发与利用。

关键词: 水稻, 稻瘟病, 抗性基因

Abstract:

【Objective】 To identify the rice blast resistance and the genotype of rice varieties in Huang-Huai-Hai Region, 【Method】the rice blast resistance of the rice varieties collected from five districts of this region was identified for two consecutive years through artificial injection and natural induction in rice blast nursery. The genotypes were analyzed with the functional markers of Pita, Pib, Pi54 and Pikm. And the cluster analysis was carried out according to the rice blast resistance level. 【Result】 85.2% and 65.9% of the tested varieties were sensitive to rice blast through artificial identification and the blast nursery identification, respectively. 84% and 65% of the tested varieties carry Pi54 and Pib, respectively. But the cluster analysis result showed that Pi54 and Pib have low contribution rate of rice blast resistance in this region.【Conclusion】 The rice varieties in the region have low resistance to rice blast, as these genes confer weaker or lost resistance to rice blast. It is crucial to screen new resistant varieties and utilize new resistant genes for breeding.

Key words: rice, rice blast, resistant gene

中图分类号:

S435.111.4⁺1

刘艳, 王宝祥, 杨波, 陈庭木, 邢运高, 孙志广, 徐波, 迟铭, 卢百关, 李健, 刘金波, 方兆伟, 秦德荣, 徐大勇. 黄淮海稻区水稻品种的穗颈瘟抗性[J]. 中国水稻科学, 2019, 33(4): 377-382.

Yan LIU, Baoxiang WANG, Bo YANG, Tingmu CHEN, Yungao XING, Zhiguang SUN, Bo XU, Ming CHI, Baiguan LU, Jian LI, Jinbo LIU, Zhaowei FANG, Derong QIN, Dayong XU. Genetic Analysis of Rice Neck Blast Resistance in Huang-Huai-Hai Region[J]. Chinese Journal OF Rice Science, 2019, 33(4): 377-382.

图/表 5

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目的基因 Target gene	引物名称 Primer name	引物序列 Sequence(5′-3′)	预期片段长 Production size/bp
Pita	Pita-F	AGCAGGTTATAAGCTAGGCC	1042
Pita	Pita-R	CTACCAACAAGTTCATCAAA	1042
pita	NPita-F	AGCAGGTTATAAGCTAGCTAT	1042
pita	NPita-R	CTACCAACAAGTTCATCAAA	1042
Pib	Pib-F	GAACAATGCCCAAACTTGAGA	365
Pib	Pib-R	GGGTCCACATGTCAGTGAGC	365
pib	NPib-F	TCGGTGCCTCGGTAGTCAGT	803
pib	NPib-R	GGGAAGCGGATCCTAGGTCT	803
Pi54	Pi54-F	CAATCTCCAAAGTTTTCAGG	216/315
Pi54	Pi54-R	GCTTCAATCACTGCTAGACC	216/315
Pikm	Pikm1-F	TGAGCTCAAGGCAAGAGTTGAGGA	174/213
Pikm	Pikm1-R	TGTTCCAGCAACTCGATGAG	174/213
pikm	Pikm2-F	CAGTAGCTGTGTCTCAGAACTATG	290/332
pikm	Pikm2-R	AAGGTACCTCTTTTCGGCCAG	290/332

目的基因 Target gene	引物名称 Primer name	引物序列 Sequence(5′-3′)	预期片段长 Production size/bp
Pita	Pita-F	AGCAGGTTATAAGCTAGGCC	1042
Pita	Pita-R	CTACCAACAAGTTCATCAAA	1042
pita	NPita-F	AGCAGGTTATAAGCTAGCTAT	1042
pita	NPita-R	CTACCAACAAGTTCATCAAA	1042
Pib	Pib-F	GAACAATGCCCAAACTTGAGA	365
Pib	Pib-R	GGGTCCACATGTCAGTGAGC	365
pib	NPib-F	TCGGTGCCTCGGTAGTCAGT	803
pib	NPib-R	GGGAAGCGGATCCTAGGTCT	803
Pi54	Pi54-F	CAATCTCCAAAGTTTTCAGG	216/315
Pi54	Pi54-R	GCTTCAATCACTGCTAGACC	216/315
Pikm	Pikm1-F	TGAGCTCAAGGCAAGAGTTGAGGA	174/213
Pikm	Pikm1-R	TGTTCCAGCAACTCGATGAG	174/213
pikm	Pikm2-F	CAGTAGCTGTGTCTCAGAACTATG	290/332
pikm	Pikm2-R	AAGGTACCTCTTTTCGGCCAG	290/332

分类 Group	抗性等级均值 Resistance grade	所占比例 Percentage / %
分类 Group	抗性等级均值 Resistance grade	Pita	Pib	Pikm	Pi54
Ⅰ	3.2	17	65	12	88
Ⅱ	3.0	0	0	0	100
Ⅲ	2.0	58	67	8	58

分类 Group	抗性等级均值 Resistance grade	所占比例 Percentage / %
分类 Group	抗性等级均值 Resistance grade	Pita	Pib	Pikm	Pi54
Ⅰ	3.2	17	65	12	88
Ⅱ	3.0	0	0	0	100
Ⅲ	2.0	58	67	8	58

抗病基因 Genotype	江苏 Jiangsu		安徽 Anhui		河南 Henan		山东 Shandong		京津地区 Beijing and Tianjin
抗病基因 Genotype	材料数 n	比例Percent /%	材料数 n	比例 Percent /%	材料数 n	比例Percent /%	材料数 n	比例Percent /%	材料数 n	比例Percent /%
无目标基因None	0	0.0	0	0.0	4	20.0	0	0.0	0	0.0
Pita	1	2.9	0	0.0	0	0.0	0	0.0	0	0.0
Pib	2	5.9	0	0.0	0	0.0	1	5.9	0	0.0
Pi54	4	11.8	1	33.3	6	30.0	2	11.8	5	35.7
Pita+Pib	2	5.9	1	33.3	0	0.0	0	0.0	3	21.4
Pita+Pi54	0	0.0	0	0.0	5	25.0	1	5.9	0	0.0
Pib+Pi54	18	52.9	0	0.0	4	20.0	9	52.9	2	14.3
Pib+Pikm	1	2.9	1	33.3	0	0.0	0	0.0	0	0.0
Pi54+Pikm	0	0.0	0	0.0	0	0.0	3	17.6	0	0.0
Pita+Pi-b+Pi54	4	11.8	0	0.0	0	0.0	1	5.9	2	14.3
Pib+Pi54+Pikm	1	2.9	0	0.0	0	0.0	0	0.0	2	14.3
Pita+Pib+Pi54+Pikm	1	2.9	0	0.0	1	5.0	0	0.0	0	0.0